This Article Full Text Full Text (PDF) Supplemental Data Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (17) Citing Articles via Google Scholar Google Scholar Articles by Saito, C. Articles by Tasaka, M. Search for Related Content PubMed PubMed Citation Articles by Saito, C. Articles by Tasaka, M. Agricola Articles by Saito, C. Articles by Tasaka, M.

Amyloplasts and Vacuolar Membrane Dynamics in the Living Graviperceptive Cell of the Arabidopsis Inflorescence Stem

Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630-0101, Japan

¹ To whom correspondence should be addressed. E-mail m-tasaka{at}bs.naist.jp; fax 81-743-72-5489.

We developed an adequate method for the in vivo analysis of organelle dynamics in the gravity-perceptive cell (endodermis) of the Arabidopsis thaliana inflorescence stem, revealing behavior of amyloplasts and vacuolar membranes in those cells. Amyloplasts in the endodermis showed saltatory movements even before gravistimulation by reorientation, and these movements were confirmed as microfilament dependent. From our quantitative analysis in the wild type, the gravity-oriented movement of amyloplasts mainly occurred during 0 to 3 min after gravistimulation by reorientation, supporting findings from our previous physiological study. Even after microfilament disruption, the gravity-oriented movement of amyloplasts remained. By contrast, in zig/sgr4 mutants, where a SNARE molecule functioning in vacuole biogenesis has been disrupted, the movement of amyloplasts in the endodermis is severely restricted both before and after gravistimulation by reorientation. Here, we describe vacuolar membrane behavior in these cells in the wild-type, actin filament–disrupted, and zig/sgr4 mutants and discuss its putatively important features for the perception of gravity. We also discuss the data on the two kinds of movements of amyloplasts that may play an important role in gravitropism: (1) the leading edge amyloplasts and (2) the en mass movement of amyloplasts.

This article has been cited by other articles:

				D. C. Bassham and M. R. Blatt SNAREs: Cogs and Coordinators in Signaling and Development Plant Physiology, August 1, 2008; 147(4): 1504 - 1515. [Full Text] [PDF]

				A. Hirota, T. Kato, H. Fukaki, M. Aida, and M. Tasaka The Auxin-Regulated AP2/EREBP Gene PUCHI Is Required for Morphogenesis in the Early Lateral Root Primordium of Arabidopsis PLANT CELL, July 1, 2007; 19(7): 2156 - 2168. [Abstract] [Full Text] [PDF]

				J. Mizoi, M. Nakamura, and I. Nishida Defects in CTP:PHOSPHORYLETHANOLAMINE CYTIDYLYLTRANSFERASE Affect Embryonic and Postembryonic Development in Arabidopsis PLANT CELL, December 1, 2006; 18(12): 3370 - 3385. [Abstract] [Full Text] [PDF]

				T. Higaki, N. Kutsuna, E. Okubo, T. Sano, and S. Hasezawa Actin Microfilaments Regulate Vacuolar Structures and Dynamics: Dual Observation of Actin Microfilaments and Vacuolar Membrane in Living Tobacco BY-2 Cells Plant Cell Physiol., July 1, 2006; 47(7): 839 - 852. [Abstract] [Full Text] [PDF]

				Z. Ma and K. H. Hasenstein The Onset of Gravisensitivity in the Embryonic Root of Flax Plant Physiology, January 1, 2006; 140(1): 159 - 166. [Abstract] [Full Text] [PDF]

				C. Limbach, J. Hauslage, C. Schafer, and M. Braun How to Activate a Plant Gravireceptor. Early Mechanisms of Gravity Sensing Studied in Characean Rhizoids during Parabolic Flights Plant Physiology, October 1, 2005; 139(2): 1030 - 1040. [Abstract] [Full Text] [PDF]

				M. Palmieri and J. Z. Kiss Disruption of the F-actin cytoskeleton limits statolith movement in Arabidopsis hypocotyls J. Exp. Bot., September 1, 2005; 56(419): 2539 - 2550. [Abstract] [Full Text] [PDF]