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First published online January 19, 2005; 10.1105/tpc.104.027540

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The Plant Cell 17:559-571 (2005)
© 2005 American Society of Plant Biologists

Genetic Analysis Reveals Domain Interactions of Arabidopsis Hsp100/ClpB and Cooperation with the Small Heat Shock Protein Chaperone System{boxw}

Ung Leea, Chris Wiea, Mindy Escobara, Ben Williamsa, Suk-Whan Hongb and Elizabeth Vierlinga,1

a Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, Arizona 85721
b Department of Applied Plant Sciences, Agricultural Plant Stress Research Center, Chonnam National University, Kwang Ju, 500-757, South Korea

1 To whom correspondence should be addressed. E-mail vierling{at}email.arizona.edu; fax 520-621-3709.

We have defined amino acids important for function of the Arabidopsis thaliana Hsp100/ClpB chaperone (AtHsp101) in acquired thermotolerance by isolating recessive, loss-of-function mutations and a novel semidominant, gain-of-function allele [hot1-4 (A499T)]. The hot1-4 allele is unusual in that it not only fails to develop thermotolerance to 45°C after acclimation at 38°C, but also is sensitive to 38°C, which is a permissive temperature for wild-type and loss-of-function mutants. hot1-4 lies between nucleotide binding domain 1 (NBD1) and NBD2 in a coiled-coil domain that is characteristic of the Hsp100/ClpB proteins. We then isolated two classes of intragenic suppressor mutations of hot1-4: loss-of-function mutations (Class 1) that eliminated the 38°C sensitivity, but did not restore thermotolerance function to hot1-4, and Class 2 suppressors that restored acquired thermotolerance function to hot1-4. Location of the hot1-4 Class 2 suppressors supports a functional link between the coiled-coil domain and both NBD1 and the axial channel of the Hsp100/ClpB hexamer. In addition, the strongest Class 2 suppressors restored solubility of aggregated small heat shock proteins (sHsps) after heat stress, revealing genetic interaction of the Hsp100/ClpB and sHsp chaperone systems. These results also demonstrate that quantitative phenotypes can be used for in vivo genetic dissection of protein mechanism in Arabidopsis.




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