This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 17/4/1205    most recent tpc.105.030775v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (17) Citing Articles via Google Scholar Google Scholar Articles by Matarasso, N. Articles by Avni, A. Search for Related Content PubMed PubMed Citation Articles by Matarasso, N. Articles by Avni, A. Agricola Articles by Matarasso, N. Articles by Avni, A.

A Novel Plant Cysteine Protease Has a Dual Function as a Regulator of 1-Aminocyclopropane-1-Carboxylic Acid Synthase Gene Expression

Department of Plant Sciences, Tel Aviv University, Tel Aviv 69978, Israel

¹ To whom correspondence should be addressed. E-mail lpavni{at}post.tau.ac.il; fax 972-3-641-0195.

The hormone ethylene influences plant growth, development, and some defense responses. The fungal elicitor Ethylene-Inducing Xylanase (EIX) elicits ethylene biosynthesis in tomato (Lycopersicon esculentum) and tobacco (Nicotiana tabacum) leaves by induction of 1-aminocyclopropane-1-caboxylic acid synthase (Acs) gene expression. A minimal promoter element in the LeAcs2 gene required for EIX responsiveness was defined by deletion analysis in transgenic tomato plants. The sequence between –715 and –675 of the tomato Acs2 gene was found to be essential for induction by EIX. A Cys protease (LeCp) was isolated that specifically binds to this cis element in vitro. Ectopic expression of LeCp in tomato leaves induced the expression of Acs2. Moreover, chromatin immunoprecipitation showed that LeCp binds in vivo to the Acs promoter. We propose a mechanism for the dual function of the LeCp protein. The protease acts enzymatically in the cytoplasm. Then, upon signaling, a small ubiquitin-related modifier protein binds to it, enabling entrance into the nucleus, where it acts as a transcription factor. Thus, LeCp can be considered a dual-function protein, having enzymatic activity and, upon elicitor signaling, exhibiting transcriptional factor activity that induces LeAcs2 expression.

This article has been cited by other articles:

				M. V. Lara, J. Borsani, C. O. Budde, M. A. Lauxmann, V. A. Lombardo, R. Murray, C. S. Andreo, and M. F. Drincovich Biochemical and proteomic analysis of 'Dixiland' peach fruit (Prunus persica) upon heat treatment J. Exp. Bot., November 1, 2009; 60(15): 4315 - 4333. [Abstract] [Full Text] [PDF]

				L. W. Neuteboom, K. O. Matsumoto, and D. A. Christopher An Extended AE-Rich N-Terminal Trunk in Secreted Pineapple Cystatin Enhances Inhibition of Fruit Bromelain and Is Posttranslationally Removed during Ripening Plant Physiology, October 1, 2009; 151(2): 515 - 527. [Abstract] [Full Text] [PDF]

				A. Wang, J. Yamakake, H. Kudo, Y. Wakasa, Y. Hatsuyama, M. Igarashi, A. Kasai, T. Li, and T. Harada Null Mutation of the MdACS3 Gene, Coding for a Ripening-Specific 1-Aminocyclopropane-1-Carboxylate Synthase, Leads to Long Shelf Life in Apple Fruit Plant Physiology, September 1, 2009; 151(1): 391 - 399. [Abstract] [Full Text] [PDF]

				S. J. Salipante, M. E. B. Rojas, B. Korkmaz, Z. Duan, J. Wechsler, K. F. Benson, R. E. Person, H. L. Grimes, and M. S. Horwitz Contributions to Neutropenia from PFAAP5 (N4BP2L2), a Novel Protein Mediating Transcriptional Repressor Cooperation between Gfi1 and Neutrophil Elastase Mol. Cell. Biol., August 15, 2009; 29(16): 4394 - 4405. [Abstract] [Full Text] [PDF]

				S. K. Tripathi, A. P. Singh, A. P. Sane, and P. Nath Transcriptional activation of a 37 kDa ethylene responsive cysteine protease gene, RbCP1, is associated with protein degradation during petal abscission in rose J. Exp. Bot., May 1, 2009; 60(7): 2035 - 2044. [Abstract] [Full Text] [PDF]

				Z. Zhang, H. Zhang, R. Quan, X.-C. Wang, and R. Huang Transcriptional Regulation of the Ethylene Response Factor LeERF2 in the Expression of Ethylene Biosynthesis Genes Controls Ethylene Production in Tomato and Tobacco Plant Physiology, May 1, 2009; 150(1): 365 - 377. [Abstract] [Full Text] [PDF]

				L. Martin, N. Leblanc-Fournier, W. Azri, C. Lenne, C. Henry, C. Coutand, and J.-L. Julien Characterization and expression analysis under bending and other abiotic factors of PtaZFP2, a poplar gene encoding a Cys2/His2 zinc finger protein Tree Physiol, January 1, 2009; 29(1): 125 - 136. [Abstract] [Full Text] [PDF]

				M. Bernoux, T. Timmers, A. Jauneau, C. Briere, P. J.G.M. de Wit, Y. Marco, and L. Deslandes RD19, an Arabidopsis Cysteine Protease Required for RRS1-R-Mediated Resistance, Is Relocalized to the Nucleus by the Ralstonia solanacearum PopP2 Effector PLANT CELL, August 1, 2008; 20(8): 2252 - 2264. [Abstract] [Full Text] [PDF]

				L. Bonneau, Y. Ge, G. E. Drury, and P. Gallois What happened to plant caspases? J. Exp. Bot., February 13, 2008; (2008) erm352v1. [Abstract] [Full Text] [PDF]

				A. K. Shukla, A. K. Shasany, M. M. Gupta, and S. P. S. Khanuja Transcriptome analysis in Catharanthus roseus leaves and roots for comparative terpenoid indole alkaloid profiles J. Exp. Bot., November 1, 2006; 57(14): 3921 - 3932. [Abstract] [Full Text] [PDF]

				F. Quattrocchio, W. Verweij, A. Kroon, C. Spelt, J. Mol, and R. Koes PH4 of Petunia Is an R2R3 MYB Protein That Activates Vacuolar Acidification through Interactions with Basic-Helix-Loop-Helix Transcription Factors of the Anthocyanin Pathway PLANT CELL, May 1, 2006; 18(5): 1274 - 1291. [Abstract] [Full Text] [PDF]