Plant Cell Hybrigenics The Protein Interactions Experts
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online April 1, 2005; 10.1105/tpc.104.026757

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
17/5/1513    most recent
tpc.104.026757v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (44)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yang, Y.-d.
Right arrow Articles by Robinson, D. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yang, Y.-d.
Right arrow Articles by Robinson, D. G.
Agricola
Right arrow Articles by Yang, Y.-d.
Right arrow Articles by Robinson, D. G.
The Plant Cell 17:1513-1531 (2005)
© 2005 American Society of Plant Biologists

Dynamics of COPII Vesicles and the Golgi Apparatus in Cultured Nicotiana tabacum BY-2 Cells Provides Evidence for Transient Association of Golgi Stacks with Endoplasmic Reticulum Exit Sites{boxw}

Yao-dong Yanga,1, Rabab Elamawib,1, Julia Bubecka, Rainer Pepperkokc, Christophe Ritzenthalerb and David G. Robinsona,2

a Department of Cell Biology, Heidelberg Institute for Plant Sciences, University of Heidelberg, 69120 Heidelberg, Germany
b Institut de Biologie Moléculaire des Plantes, 67084 Strasbourg, France
c Cell Biology Cell Biophysics Programme, European Molecular Biology Laboratory, 69117 Heidelberg, Germany

2 To whom correspondence should be addressed. E-mail david.robinson{at}urz.uni-heidelberg.de; fax 49-6221-546406.

Despite the ubiquitous presence of the COPI, COPII, and clathrin vesicle budding machineries in all eukaryotes, the organization of the secretory pathway in plants differs significantly from that in yeast and mammalian cells. Mobile Golgi stacks and the lack of both transitional endoplasmic reticulum (ER) and a distinct ER-to-Golgi intermediate compartment are the most prominent distinguishing morphological features of the early secretory pathway in plants. Although the formation of COPI vesicles at periphery of Golgi cisternae has been demonstrated in plants, exit from the ER has been difficult to visualize, and the spatial relationship of this event is now a matter of controversy. Using tobacco (Nicotiana tabacum) BY-2 cells, which represent a highly active secretory system, we have used two approaches to investigate the location and dynamics of COPII binding to the ER and the relationship of these ER exit sites (ERES) to the Golgi apparatus. On the one hand, we have identified endogenous COPII using affinity purified antisera generated against selected COPII-coat proteins (Sar1, Sec13, and Sec23); on the other hand, we have prepared a BY-2 cell line expressing Sec13:green fluorescent protein (GFP) to perform live cell imaging with red fluorescent protein–labeled ER or Golgi stacks. COPII binding to the ER in BY-2 cells is visualized as fluorescent punctate structures uniformly distributed over the surface of the ER, both after antibody staining as well as by Sec13:GFP expression. These structures are smaller and greatly outnumber the Golgi stacks. They are stationary, but have an extremely short half-life (<10 s). Without correlative imaging data on the export of membrane or lumenal ER cargo it was not possible to equate unequivocally these COPII binding loci with ERES. When a GDP-fixed Sar1 mutant is expressed, ER export is blocked and the visualization of COPII binding is perturbed. On the other hand, when secretion is inhibited by brefeldin A, COPII binding sites on the ER remain visible even after the Golgi apparatus has been lost. Live cell imaging in a confocal laser scanning microscope equipped with spinning disk optics allowed us to investigate the relationship between mobile Golgi stacks and COPII binding sites. As they move, Golgi stacks temporarily associated with COPII binding sites at their rims. Golgi stacks were visualized with their peripheries partially or fully occupied with COPII. In the latter case, Golgi stacks had the appearance of a COPII halo. Slow moving Golgi stacks tended to have more peripheral COPII than faster moving ones. However, some stationary Golgi stacks entirely lacking COPII were also observed. Our results indicate that, in a cell type with highly mobile Golgi stacks like tobacco BY-2, the Golgi apparatus is not continually linked to a single ERES. By contrast, Golgi stacks associate intermittently and sometimes concurrently with several ERES as they move.




This article has been cited by other articles:


Home page
 J. Virol.Home page
T. Wei and A. Wang
Biogenesis of Cytoplasmic Membranous Vesicles for Plant Potyvirus Replication Occurs at Endoplasmic Reticulum Exit Sites in a COPI- and COPII-Dependent Manner
J. Virol., December 15, 2008; 82(24): 12252 - 12264.
[Abstract] [Full Text] [PDF]

Home page
 Mol PlantHome page
M. Dunkel, A. Latz, K. Schumacher, T. Muller, D. Becker, and R. Hedrich
Targeting of Vacuolar Membrane Localized Members of the TPK Channel Family
Mol Plant, November 1, 2008; 1(6): 938 - 949.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
F. Alkhalfioui, M. Renard, P. Frendo, C. Keichinger, Y. Meyer, E. Gelhaye, M. Hirasawa, D. B. Knaff, C. Ritzenthaler, and F. Montrichard
A Novel Type of Thioredoxin Dedicated to Symbiosis in Legumes
Plant Physiology, September 1, 2008; 148(1): 424 - 435.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
L. A. Staehelin and B.-H. Kang
Nanoscale Architecture of Endoplasmic Reticulum Export Sites and of Golgi Membranes as Determined by Electron Tomography
Plant Physiology, August 1, 2008; 147(4): 1454 - 1468.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. A. Held, A. Boulaflous, and F. Brandizzi
Advances in Fluorescent Protein-Based Imaging for the Analysis of Plant Endomembranes
Plant Physiology, August 1, 2008; 147(4): 1469 - 1481.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
E. Nielsen, A. Y. Cheung, and T. Ueda
The Regulatory RAB and ARF GTPases for Vesicular Trafficking
Plant Physiology, August 1, 2008; 147(4): 1516 - 1526.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Langhans, C. Hawes, S. Hillmer, E. Hummel, and D. G. Robinson
Golgi Regeneration after Brefeldin A Treatment in BY-2 Cells Entails Stack Enlargement and Cisternal Growth followed by Division
Plant Physiology, October 1, 2007; 145(2): 527 - 538.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. K. Karnik and R. N. Trelease
Arabidopsis peroxin 16 trafficks through the ER and an intermediate compartment to pre-existing peroxisomes via overlapping molecular targeting signals
J. Exp. Bot., May 1, 2007; 58(7): 1677 - 1693.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. L. Hanton, L. Chatre, L. Renna, L. A. Matheson, and F. Brandizzi
De Novo Formation of Plant Endoplasmic Reticulum Export Sites Is Membrane Cargo Induced and Signal Mediated
Plant Physiology, April 1, 2007; 143(4): 1640 - 1650.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
H.-J. Ju, J. E. Brown, C.-M. Ye, and J. Verchot-Lubicz
Mutations in the Central Domain of Potato Virus X TGBp2 Eliminate Granular Vesicles and Virus Cell-to-Cell Trafficking
J. Virol., February 15, 2007; 81(4): 1899 - 1911.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
B. S. Donohoe, B.-H. Kang, and L. A. Staehelin
Identification and characterization of COPIa- and COPIb-type vesicle classes associated with plant and algal Golgi
PNAS, January 2, 2007; 104(1): 163 - 168.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
P. Moreau, F. Brandizzi, S. Hanton, L. Chatre, S. Melser, C. Hawes, and B. Satiat-Jeunemaitre
The plant ER-Golgi interface: a highly structured and dynamic membrane complex
J. Exp. Bot., January 1, 2007; 58(1): 49 - 64.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Y. Miao, P. K. Yan, H. Kim, I. Hwang, and L. Jiang
Localization of Green Fluorescent Protein Fusions with the Seven Arabidopsis Vacuolar Sorting Receptors to Prevacuolar Compartments in Tobacco BY-2 Cells
Plant Physiology, November 1, 2006; 142(3): 945 - 962.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
J. Ashby, E. Boutant, M. Seemanpillai, A. Sambade, C. Ritzenthaler, and M. Heinlein
Tobacco mosaic virus movement protein functions as a structural microtubule-associated protein.
J. Virol., September 1, 2006; 80(17): 8329 - 8344.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. Song, M. H. Lee, G.-J. Lee, C. M. Yoo, and I. Hwang
Arabidopsis EPSIN1 Plays an Important Role in Vacuolar Trafficking of Soluble Cargo Proteins in Plant Cells via Interactions with Clathrin, AP-1, VTI11, and VSR1
PLANT CELL, September 1, 2006; 18(9): 2258 - 2274.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
P. Oliviusson, O. Heinzerling, S. Hillmer, G. Hinz, Y. C. Tse, L. Jiang, and D. G. Robinson
Plant Retromer, Localized to the Prevacuolar Compartment and Microvesicles in Arabidopsis, May Interact with Vacuolar Sorting Receptors
PLANT CELL, May 1, 2006; 18(5): 1239 - 1252.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
J. Runions, T. Brach, S. Kuhner, and C. Hawes
Photoactivation of GFP reveals protein dynamics within the endoplasmic reticulum membrane
J. Exp. Bot., January 1, 2006; 57(1): 43 - 50.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
L. Chatre, F. Brandizzi, A. Hocquellet, C. Hawes, and P. Moreau
Sec22 and Memb11 Are v-SNAREs of the Anterograde Endoplasmic Reticulum-Golgi Pathway in Tobacco Leaf Epidermal Cells
Plant Physiology, November 1, 2005; 139(3): 1244 - 1254.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2005 by the American Society of Plant Biologists