Plant Cell Applied Biosystems SYBR(R) Cells-to-CT(TM) Kits
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online May 6, 2005; 10.1105/tpc.105.031823

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
17/6/1788    most recent
tpc.105.031823v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (17)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sagi, G.
Right arrow Articles by Epel, B. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sagi, G.
Right arrow Articles by Epel, B. L.
Agricola
Right arrow Articles by Sagi, G.
Right arrow Articles by Epel, B. L.
The Plant Cell 17:1788-1800 (2005)
© 2005 American Society of Plant Biologists

Class 1 Reversibly Glycosylated Polypeptides Are Plasmodesmal-Associated Proteins Delivered to Plasmodesmata via the Golgi Apparatus{boxw}

Guy Sagi1, Aviva Katz1, Dana Guenoune-Gelbart and Bernard L. Epel2

Department of Plant Sciences, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel

2 To whom correspondence should be addressed. E-mail blepel{at}post.tau.ac.il; fax 972-3-6409380.

SE-WAP41, a salt-extractable 41-kD wall-associated protein that is associated with walls of etiolated maize (Zea mays) seedlings and is recognized by an antiserum previously reported to label plasmodesmata and the Golgi, was cloned, sequenced, and found to be a class 1 reversibly glycosylated polypeptide (C1RGP). Protein gel blot analysis of cell fractions with an antiserum against recombinant SE-WAP41 showed it to be enriched in the wall fraction. RNA gel blot analysis along the mesocotyl developmental axis and during deetiolation demonstrates that high SE-WAP41 transcript levels correlate spatially and temporally with primary and secondary plasmodesmata (Pd) formation. All four of the Arabidopsis thaliana C1RGP proteins, when fused to green fluorescent protein (GFP) and transiently expressed in tobacco (Nicotiana tabacum) epidermal cells, display fluorescence patterns indicating they are Golgi- and plasmodesmal-associated proteins. Localization to the Golgi apparatus was verified by colocalization of transiently expressed AtRGP2 fused to cyan fluorescence protein together with a known Golgi marker, Golgi Nucleotide Sugar Transporter 1 fused to yellow fluorescent protein (GONST1:YFP). In transgenic tobacco, AtRGP2:GFP fluorescence is punctate, is present only in contact walls between cells, and colocalizes with aniline blue–stained callose present around Pd. In plasmolyzed cells, AtRGP2:GFP remains wall embedded, whereas GONST1:YFP cannot be found embedded in cell walls. This result implies that the targeting to Pd is not due to a default pathway for Golgi-localized fusion proteins but is specific to C1RGPs. Treatment with the Golgi disrupting drug Brefeldin A inhibits Pd labeling by AtRGP2:GFP. Integrating these data, we conclude that C1RGPs are plasmodesmal-associated proteins delivered to plasmodesmata via the Golgi apparatus.




This article has been cited by other articles:


Home page
 Plant CellHome page
C. Faulkner, O. E. Akman, K. Bell, C. Jeffree, and K. Oparka
Peeking into Pit Fields: A Multiple Twinning Model of Secondary Plasmodesmata Formation in Tobacco
PLANT CELL, June 1, 2008; 20(6): 1504 - 1518.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
D. Avisar, A. I. Prokhnevsky, and V. V. Dolja
Class VIII Myosins Are Required for Plasmodesmatal Localization of a Closterovirus Hsp70 Homolog
J. Virol., March 15, 2008; 82(6): 2836 - 2843.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
T. Konishi, T. Takeda, Y. Miyazaki, M. Ohnishi-Kameyama, T. Hayashi, M. A. O'Neill, and T. Ishii
A plant mutase that interconverts UDP-arabinofuranose and UDP-arabinopyranose
Glycobiology, March 1, 2007; 17(3): 345 - 354.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. Drakakaki, O. Zabotina, I. Delgado, S. Robert, K. Keegstra, and N. Raikhel
Arabidopsis Reversibly Glycosylated Polypeptides 1 and 2 Are Essential for Pollen Development
Plant Physiology, December 1, 2006; 142(4): 1480 - 1492.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
J. Ferralli, J. Ashby, M. Fasler, V. Boyko, and M. Heinlein
Disruption of Microtubule Organization and Centrosome Function by Expression of Tobacco Mosaic Virus Movement Protein.
J. Virol., June 1, 2006; 80(12): 5807 - 5821.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
A. I. Prokhnevsky, V. V. Peremyslov, and V. V. Dolja
Actin Cytoskeleton Is Involved in Targeting of a Viral Hsp70 Homolog to the Cell Periphery
J. Virol., November 15, 2005; 79(22): 14421 - 14428.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. S. Nelson and V. Citovsky
Plant Viruses. Invaders of Cells and Pirates of Cellular Pathways
Plant Physiology, August 1, 2005; 138(4): 1809 - 1814.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
P. Boevink and K. J. Oparka
Virus-Host Interactions during Movement Processes
Plant Physiology, August 1, 2005; 138(4): 1815 - 1821.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2005 by the American Society of Plant Biologists