Plant Cell Huazhong Agricultural University
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online May 27, 2005; 10.1105/tpc.105.031856

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
17/7/2037    most recent
tpc.105.031856v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in Plant Cell
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (38)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pracharoenwattana, I.
Right arrow Articles by Smith, S. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pracharoenwattana, I.
Right arrow Articles by Smith, S. M.
Agricola
Right arrow Articles by Pracharoenwattana, I.
Right arrow Articles by Smith, S. M.
The Plant Cell 17:2037-2048 (2005)
© 2005 American Society of Plant Biologists

Arabidopsis Peroxisomal Citrate Synthase Is Required for Fatty Acid Respiration and Seed Germination{boxw}

Itsara Pracharoenwattana, Johanna E. Cornah1 and Steven M. Smith2,3

Institute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3JH, United Kingdom

3 To whom correspondence should be addressed. E-mail ssmith{at}cyllene.uwa.edu.au; fax 61-8-6488-4404.

We tested the hypothesis that peroxisomal citrate synthase (CSY) is required for carbon transfer from peroxisomes to mitochondria during respiration of triacylglycerol in Arabidopsis thaliana seedlings. Two genes encoding peroxisomal CSY are expressed in Arabidopsis seedlings, and seeds from plants with both CSY genes disrupted were dormant and did not metabolize triacylglycerol. Germination was achieved by removing the seed coat and supplying sucrose, but the seedlings still did not use triacylglycerol. The mutant seedlings were resistant to 2,4-dichlorophenoxybutyric acid, indicating a block in peroxisomal ß-oxidation, and were unable to develop further after transfer to soil. The mutant phenotype was complemented with a cDNA encoding CSY with either its native peroxisomal targeting sequence (PTS2) or a heterologous PTS1 sequence from pumpkin (Cucurbita pepo) malate synthase. These results suggest that peroxisomal CSY in Arabidopsis is not only a key enzyme of the glyoxylate cycle but also catalyzes an essential step in the respiration of fatty acids. We conclude that citrate is exported from the peroxisome during fatty acid respiration, whereas in yeast, acetylcarnitine is exported.


Related articles in Plant Cell:

Peroxisomal Citrate Synthase Provides Exit Route from Fatty Acid Metabolism in Oilseeds
Nancy A. Eckardt
Plant Cell 2005 17: 1863-1865. [Full Text]  



This article has been cited by other articles:


Home page
 Mol PlantHome page
N. Linka and A. P.M. Weber
Intracellular Metabolite Transporters in Plants
Mol Plant, January 1, 2010; 3(1): 21 - 53.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. Schluter, A. Real-Chicharro, T. Gabaldon, F. Sanchez-Jimenez, and A. Pujol
PeroxisomeDB 2.0: an integrative view of the global peroxisomal metabolome
Nucleic Acids Res., January 1, 2010; 38(suppl_1): D800 - D805.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Engqvist, M. F. Drincovich, U.-I. Flugge, and V. G. Maurino
Two D-2-Hydroxy-acid Dehydrogenases in Arabidopsis thaliana with Catalytic Capacities to Participate in the Last Reactions of the Methylglyoxal and {beta}-Oxidation Pathways
J. Biol. Chem., September 11, 2009; 284(37): 25026 - 25037.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
H.-H. Kunz, M. Scharnewski, K. Feussner, I. Feussner, U.-I. Flugge, M. Fulda, and M. Gierth
The ABC Transporter PXA1 and Peroxisomal {beta}-Oxidation Are Vital for Metabolism in Mature Leaves of Arabidopsis during Extended Darkness
PLANT CELL, September 1, 2009; 21(9): 2733 - 2749.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. Penfield and A. Hall
A Role for Multiple Circadian Clock Genes in the Response to Signals That Break Seed Dormancy in Arabidopsis
PLANT CELL, June 1, 2009; 21(6): 1722 - 1732.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
D. Dietrich, H. Schmuths, C. D. M. Lousa, J. M. Baldwin, S. A. Baldwin, A. Baker, F. L. Theodoulou, and M. J. Holdsworth
Mutations in the Arabidopsis Peroxisomal ABC Transporter COMATOSE Allow Differentiation between Multiple Functions In Planta: Insights from an Allelic Series
Mol. Biol. Cell, January 1, 2009; 20(1): 530 - 543.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
N. Linka, F. L. Theodoulou, R. P. Haslam, M. Linka, J. A. Napier, H. E. Neuhaus, and A. P.M. Weber
Peroxisomal ATP Import Is Essential for Seedling Development in Arabidopsis thaliana
PLANT CELL, December 1, 2008; 20(12): 3241 - 3257.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
H. Eubel, E. H. Meyer, N. L. Taylor, J. D. Bussell, N. O'Toole, J. L. Heazlewood, I. Castleden, I. D. Small, S. M. Smith, and A. H. Millar
Novel Proteins, Putative Membrane Transporters, and an Integrated Metabolic Network Are Revealed by Quantitative Proteomic Analysis of Arabidopsis Cell Culture Peroxisomes
Plant Physiology, December 1, 2008; 148(4): 1809 - 1829.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
B. S. Zheng, E. Ronnberg, L. Viitanen, T. A. Salminen, K. Lundgren, T. Moritz, and J. Edqvist
Arabidopsis sterol carrier protein-2 is required for normal development of seeds and seedlings
J. Exp. Bot., September 1, 2008; 59(12): 3485 - 3499.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Sienkiewicz-Porzucek, A. Nunes-Nesi, R. Sulpice, J. Lisec, D. C. Centeno, P. Carillo, A. Leisse, E. Urbanczyk-Wochniak, and A. R. Fernie
Mild Reductions in Mitochondrial Citrate Synthase Activity Result in a Compromised Nitrate Assimilation and Reduced Leaf Pigmentation But Have No Effect on Photosynthetic Performance or Growth
Plant Physiology, May 1, 2008; 147(1): 115 - 127.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
K. Strijbis, C. W. T. van Roermund, W. F. Visser, E. C. Mol, J. van den Burg, D. M. MacCallum, F. C. Odds, E. Paramonova, B. P. Krom, and B. Distel
Carnitine-Dependent Transport of Acetyl Coenzyme A in Candida albicans Is Essential for Growth on Nonfermentable Carbon Sources and Contributes to Biofilm Formation
Eukaryot. Cell, April 1, 2008; 7(4): 610 - 618.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Andre and C. Benning
Arabidopsis Seedlings Deficient in a Plastidic Pyruvate Kinase Are Unable to Utilize Seed Storage Compounds for Germination and Establishment
Plant Physiology, December 1, 2007; 145(4): 1670 - 1680.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Footitt, J. E. Cornah, I. Pracharoenwattana, J. H. Bryce, and S. M. Smith
The Arabidopsis 3-ketoacyl-CoA thiolase-2 (kat2-1) mutant exhibits increased flowering but reduced reproductive success
J. Exp. Bot., August 28, 2007; (2007) erm146v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Footitt, D. Dietrich, A. Fait, A. R. Fernie, M. J. Holdsworth, A. Baker, and F. L. Theodoulou
The COMATOSE ATP-Binding Cassette Transporter Is Required for Full Fertility in Arabidopsis
Plant Physiology, July 1, 2007; 144(3): 1467 - 1480.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
J. E. Lunn
Compartmentation in plant metabolism
J. Exp. Bot., January 1, 2007; 58(1): 35 - 47.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. W. Murcha, D. Elhafez, R. Lister, J. Tonti-Filippini, M. Baumgartner, K. Philippar, C. Carrie, D. Mokranjac, J. Soll, and J. Whelan
Characterization of the Preprotein and Amino Acid Transporter Gene Family in Arabidopsis
Plant Physiology, January 1, 2007; 143(1): 199 - 212.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Footitt, J. Marquez, H. Schmuths, A. Baker, F. L. Theodoulou, and M. Holdsworth
Analysis of the role of COMATOSE and peroxisomal beta-oxidation in the determination of germination potential in Arabidopsis
J. Exp. Bot., August 1, 2006; 57(11): 2805 - 2814.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Rubio, T. R. Larson, M. Gonzalez-Guzman, S. Alejandro, I. A. Graham, R. Serrano, and P. L. Rodriguez
An Arabidopsis Mutant Impaired in Coenzyme A Biosynthesis Is Sugar Dependent for Seedling Establishment
Plant Physiology, March 1, 2006; 140(3): 830 - 843.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
N. A. Eckardt
Peroxisomal Citrate Synthase Provides Exit Route from Fatty Acid Metabolism in Oilseeds
PLANT CELL, July 1, 2005; 17(7): 1863 - 1865.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2005 by the American Society of Plant Biologists