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First published online December 2, 2005; 10.1105/tpc.105.036392

The Plant Cell 18:176-197 (2006)
© 2006 American Society of Plant Biologists

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pTAC2, -6, and -12 Are Components of the Transcriptionally Active Plastid Chromosome That Are Required for Plastid Gene Expression

Jeannette Pfalza, Karsten Liereb, Andrea Kandlbinderc, Karl-Josef Dietzc and Ralf Oelmüllera,1

a Institute of General Botany and Plant Physiology, Friedrich-Schiller-University, 07743 Jena, Germany
b Institute of Biology (Genetics), Humboldt University, D-10115 Berlin, Germany
c Department of Plant Physiology and Biochemistry, University of Bielefeld, 33501 Bielefeld, Germany

1 To whom correspondence should be addressed. E-mail b7oera{at}uni-jena.de; fax 49-3641-949230.

Transcription in plastids is mediated by a plastid-encoded multimeric (PEP) and a nuclear-encoded single-subunit RNA polymerase (NEP) and a still unknown number of nuclear-encoded factors. By combining gel filtration and affinity chromatography purification steps, we isolated transcriptionally active chromosomes from Arabidopsis thaliana and mustard (Sinapis alba) chloroplasts and identified 35 components by electrospray ionization ion trap tandem mass spectrometry. Eighteen components, called plastid transcriptionally active chromosome proteins (pTACs), have not yet been described. T-DNA insertions in three corresponding genes, ptac2, -6, and -12, are lethal without exogenous carbon sources. Expression patterns of the plastid-encoded genes in the corresponding knockout lines resemble those of {Delta}rpo mutants. For instance, expression of plastid genes with PEP promoters is downregulated, while expression of genes with NEP promoters is either not affected or upregulated in the mutants. All three components might also be involved in posttranscriptional processes, such as RNA processing and/or mRNA stability. Thus, pTAC2, -6, and -12 are clearly involved in plastid gene expression.




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