This Article Full Text Full Text (PDF) All Versions of this Article: 18/10/2431    most recent tpc.106.045088v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (11) Citing Articles via Google Scholar Google Scholar Articles by Kirik, A. Articles by Reiss, B. Search for Related Content PubMed PubMed Citation Articles by Kirik, A. Articles by Reiss, B. Agricola Articles by Kirik, A. Articles by Reiss, B.

The Chromatin Assembly Factor Subunit FASCIATA1 Is Involved in Homologous Recombination in Plants

^a Department of Plant Developmental Biology, Max-Planck-Institut für Züchtungsforschung, 50829 Cologne, Germany
^b Department of Cytogenetics, Institute of Plant Genetics and Crop Plant Research, 06466 Gatersleben, Germany

³ To whom correspondence should be addressed. E-mail reiss{at}mpiz-koeln.mpg.de; fax 49-221-5062213.

DNA replication in cycling eukaryotic cells necessitates the reestablishment of chromatin after nucleosome redistribution from the parental to the two daughter DNA strands. Chromatin assembly factor 1 (CAF-1), a heterotrimeric complex consisting of three subunits (p150/p60/p48), is one of the replication-coupled assembly factors involved in the reconstitution of S-phase chromatin. CAF-1 is required in vitro for nucleosome assembly onto newly replicated chromatin in human cells and Arabidopsis thaliana, and defects in yeast (Saccharomyces cerevisiae) affect DNA damage repair processes, predominantly those involved in genome stability. However, in vivo chromatin defects of caf-1 mutants in higher eukaryotes are poorly characterized. Here, we show that fasciata1-4 (fas1-4), a new allele of the Arabidopsis fas1 mutant defective in the p150 subunit of CAF-1, has a severe developmental phenotype, reduced heterochromatin content, and a more open conformation of euchromatin. Most importantly, homologous recombination (HR), a process involved in maintaining genome stability, is increased dramatically in fas1-4, as indicated by a 96-fold stimulation of intrachromosomal HR. Together with the open conformation of chromatin and the nearly normal expression levels of HR genes in the mutant, this result suggests that chromatin is a major factor restricting HR in plants.

This article has been cited by other articles:

				D. Schuermann, O. Fritsch, J. M. Lucht, and B. Hohn Replication Stress Leads to Genome Instabilities in Arabidopsis DNA Polymerase {delta} Mutants PLANT CELL, September 1, 2009; 21(9): 2700 - 2714. [Abstract] [Full Text] [PDF]

				H. Yin, X. Zhang, J. Liu, Y. Wang, J. He, T. Yang, X. Hong, Q. Yang, and Z. Gong Epigenetic Regulation, Somatic Homologous Recombination, and Abscisic Acid Signaling Are Influenced by DNA Polymerase {epsilon} Mutation in Arabidopsis PLANT CELL, February 1, 2009; 21(2): 386 - 402. [Abstract] [Full Text] [PDF]

				N. Kornet and B. Scheres Stem Cell Factors in Plants: Chromatin Connections Cold Spring Harb Symp Quant Biol, January 15, 2009; (2009) sqb.2008.73.043v1. [Abstract] [PDF]

				K. Dohke, S. Miyazaki, K. Tanaka, T. Urano, S. I. S. Grewal, and Y. Murakami Fission yeast chromatin assembly factor 1 assists in the replication-coupled maintenance of heterochromatin Genes Cells, October 1, 2008; 13(10): 1027 - 1043. [Abstract] [Full Text] [PDF]

				Z. Chen, J. L. H. Tan, M. Ingouff, V. Sundaresan, and F. Berger Chromatin assembly factor 1 regulates the cell cycle but not cell fate during male gametogenesis in Arabidopsis thaliana Development, January 1, 2008; 135(1): 65 - 73. [Abstract] [Full Text] [PDF]

				V. Kirik, A. Schrader, J. F. Uhrig, and M. Hulskamp MIDGET Unravels Functions of the Arabidopsis Topoisomerase VI Complex in DNA Endoreduplication, Chromatin Condensation, and Transcriptional Silencing PLANT CELL, October 1, 2007; 19(10): 3100 - 3110. [Abstract] [Full Text] [PDF]

				R. Terada, Y. Johzuka-Hisatomi, M. Saitoh, H. Asao, and S. Iida Gene Targeting by Homologous Recombination as a Biotechnological Tool for Rice Functional Genomics Plant Physiology, June 1, 2007; 144(2): 846 - 856. [Abstract] [Full Text] [PDF]

				A. Anand, A. Krichevsky, S. Schornack, T. Lahaye, T. Tzfira, Y. Tang, V. Citovsky, and K. S. Mysore Arabidopsis VIRE2 INTERACTING PROTEIN2 Is Required for Agrobacterium T-DNA Integration in Plants PLANT CELL, May 1, 2007; 19(5): 1695 - 1708. [Abstract] [Full Text] [PDF]

				E. Ramirez-Parra and C. Gutierrez E2F Regulates FASCIATA1, a Chromatin Assembly Gene Whose Loss Switches on the Endocycle and Activates Gene Expression by Changing the Epigenetic Status Plant Physiology, May 1, 2007; 144(1): 105 - 120. [Abstract] [Full Text] [PDF]