Plant Cell Illumina, Inc.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online October 6, 2006; 10.1105/tpc.106.043174

The Plant Cell 18:2443-2451 (2006)
© 2006 American Society of Plant Biologists

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow PPT slides of all figures
Right arrow All Versions of this Article:
18/10/2443    most recent
tpc.106.043174v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (13)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lermontova, I.
Right arrow Articles by Schubert, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lermontova, I.
Right arrow Articles by Schubert, I.
Agricola
Right arrow Articles by Lermontova, I.
Right arrow Articles by Schubert, I.

Loading of Arabidopsis Centromeric Histone CENH3 Occurs Mainly during G2 and Requires the Presence of the Histone Fold Domain

Inna Lermontovaa,1, Veit Schuberta, Joerg Fuchsa, Sabina Klattea, Jiri Macasb and Ingo Schuberta

a Leibniz Institute of Plant Genetics and Crop Plant Research, D-06466 Gatersleben, Germany
b Institute of Plant Molecular Biology, CZ-37005 Ceske Budejovice, Czech Republic

1 To whom correspondence should be addressed. E-mail lermonto{at}ipk-gatersleben.de; fax 49-39-482-5137.

The centromeric histone H3 (CENH3) substitutes histone H3 within the nucleosomes of active centromeres in all eukaryotes. CENH3 deposition at centromeres is needed to assemble the kinetochore, a complex of conserved proteins responsible for correct chromosome segregation during nuclear division. Histones of regular nucleosomes are loaded during replication in S phase, while CENH3 deposition deviates from this pattern in yeast, human, and Drosophila melanogaster cells. Little is known about when and how CENH3 targets centromeric loci. Therefore, we determined the location and quantity of recombinant enhanced yellow fluorescent protein (EYFP)-CENH3 in mitotic root and endopolyploid leaf nuclei of transgenic Arabidopsis thaliana cells. Our data indicate significant loading of A. thaliana CENH3 during G2 (before splitting into sister kinetochores) rather than during the S or M phase of the cell cycle. The histone fold domain of the C-terminal part of CENH3 is sufficient to target A. thaliana centromeres. A. thaliana EYFP-CENH3 can recognize and target three different centromeric repeats of Arabidopsis lyrata but not field bean (Vicia faba) centromeres.




This article has been cited by other articles:


Home page
 Plant CellHome page
F. Han, Z. Gao, and J. A. Birchler
Reactivation of an Inactive Centromere Reveals Epigenetic and Structural Components for Centromere Specification in Maize
PLANT CELL, July 1, 2009; 21(7): 1929 - 1939.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
M. Dominguez and F. Berger
Chromatin and the cell cycle meet in Madrid
Development, November 1, 2008; 135(21): 3475 - 3480.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
P. Hemmerich, S. Weidtkamp-Peters, C. Hoischen, L. Schmiedeberg, I. Erliandri, and S. Diekmann
Dynamics of inner kinetochore assembly and maintenance in living cells
J. Cell Biol., March 24, 2008; 180(6): 1101 - 1114.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Dalal, T. Furuyama, D. Vermaak, and S. Henikoff
Inaugural Article: Structure, dynamics, and evolution of centromeric nucleosomes
PNAS, October 9, 2007; 104(41): 15974 - 15981.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Furuyama and S. Biggins
Centromere identity is specified by a single centromeric nucleosome in budding yeast
PNAS, September 11, 2007; 104(37): 14706 - 14711.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2006 by the American Society of Plant Biologists