First published online November 30, 2006; 10.1105/tpc.106.044107
The Plant Cell 18:2999-3014 (2006)
© 2006 American Society of Plant Biologists
The Rice Tapetum Degeneration Retardation Gene Is Required for Tapetum Degradation and Anther Development[W]
Na Lia,b,1,
Da-Sheng Zhanga,1,
Hai-Sheng Liua,
Chang-Song Yina,
Xiao-xing Lia,
Wan-qi Lianga,
Zheng Yuana,
Ben Xuc,
Huang-Wei Chua,
Jia Wanga,
Tie-Qiao Wenb,
Hai Huangc,
Da Luoc,
Hong Maa,c,d and
Da-Bing Zhanga,c,2
a Shanghai Jiao Tong University–Shanghai Institutes for Biological Sciences–Pennsylvania State University Joint Center for Life Sciences, School of Life Science and Biotechnology, Key Laboratory of Microbial Metabolism, Ministry of Education, Shanghai Jiao Tong University, Shanghai 200240, China
b College of Life Science, Shanghai University, Shanghai 200436, China
c Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China
d Department of Biology, Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, Pennsylvania 16082
2 To whom correspondence should be addressed. E-mail zhangdb{at}sjtu.edu.cn; fax 86-21-34204869.
In flowering plants, tapetum degeneration is proposed to be triggered by a programmed cell death (PCD) process during late stages of pollen development; the PCD is thought to provide cellular contents supporting pollen wall formation and to allow the subsequent pollen release. However, the molecular basis regulating tapetum PCD in plants remains poorly understood. We report the isolation and characterization of a rice (Oryza sativa) male sterile mutant tapetum degeneration retardation (tdr), which exhibits degeneration retardation of the tapetum and middle layer as well as collapse of microspores. The TDR gene is preferentially expressed in the tapetum and encodes a putative basic helix-loop-helix protein, which is likely localized to the nucleus. More importantly, two genes, Os CP1 and Os c6, encoding a Cys protease and a protease inhibitor, respectively, were shown to be the likely direct targets of TDR through chromatin immunoprecipitation analyses and the electrophoretic mobility shift assay. These results indicate that TDR is a key component of the molecular network regulating rice tapetum development and degeneration.
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