This Article Full Text Full Text (PDF) PPT slides of all figures Supplemental Data All Versions of this Article: 18/12/3617    most recent tpc.106.044743v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in Plant Cell Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (11) Citing Articles via Google Scholar Google Scholar Articles by Deeken, R. Articles by Hedrich, R. Search for Related Content PubMed PubMed Citation Articles by Deeken, R. Articles by Hedrich, R. Agricola Articles by Deeken, R. Articles by Hedrich, R.

An Integrated View of Gene Expression and Solute Profiles of Arabidopsis Tumors: A Genome-Wide Approach^[W]

^a Julius-von-Sachs-Institute, Department of Molecular Plant Physiology and Biophysics, University of Wuerzburg, D-97082 Wuerzburg, Germany
^b Theodor-Boveri-Institute, Department of Bioinformatics, University of Wuerzburg, D-97074 Wuerzburg, Germany
^c Institute of Biology II, Cell Biology, University of Freiburg, 79104 Freiburg, Germany
^d Julius-von-Sachs-Institute, Department of Pharmaceutical Biology, University of Wuerzburg, D-97082 Wuerzburg, Germany

¹ To whom correspondence should be addressed. E-mail hedrich{at}botanik.uni-wuerzburg.de; fax 49-391-888-6157.

Transformation of plant cells with T-DNA of virulent agrobacteria is one of the most extreme triggers of developmental changes in higher plants. For rapid growth and development of resulting tumors, specific changes in the gene expression profile and metabolic adaptations are required. Increased transport and metabolic fluxes are critical preconditions for growth and tumor development. A functional genomics approach, using the Affymetrix whole genome microarray (22,800 genes), was applied to measure changes in gene expression. The solute pattern of Arabidopsis thaliana tumors and uninfected plant tissues was compared with the respective gene expression profile. Increased levels of anions, sugars, and amino acids were correlated with changes in the gene expression of specific enzymes and solute transporters. The expression profile of genes pivotal for energy metabolism, such as those involved in photosynthesis, mitochondrial electron transport, and fermentation, suggested that tumors produce C and N compounds heterotrophically and gain energy mainly anaerobically. Thus, understanding of gene-to-metabolite networks in plant tumors promotes the identification of mechanisms that control tumor development.

Related articles in Plant Cell:

A Genomic Analysis of Tumor Development and Source-Sink Relationships in Agrobacterium-Induced Crown Gall Disease in Arabidopsis

Nancy A. Eckardt
Plant Cell 2006 18: 3350-3352. [Full Text]  

This article has been cited by other articles:

				C.-W. Lee, M. Efetova, J. C Engelmann, R. Kramell, C. Wasternack, J. Ludwig-Muller, R. Hedrich, and R. Deeken Agrobacterium tumefaciens Promotes Tumor Induction by Modulating Pathogen Defense in Arabidopsis thaliana PLANT CELL, September 1, 2009; 21(9): 2948 - 2962. [Abstract] [Full Text] [PDF]

				E. Haudecoeur, S. Planamente, A. Cirou, M. Tannieres, B. J. Shelp, S. Morera, and D. Faure Proline antagonizes GABA-induced quenching of quorum-sensing in Agrobacterium tumefaciens PNAS, August 25, 2009; 106(34): 14587 - 14592. [Abstract] [Full Text] [PDF]

				S. B. Gelvin Agrobacterium in the Genomics Age Plant Physiology, August 1, 2009; 150(4): 1665 - 1676. [Full Text] [PDF]

				M. R. Aluru, J. Zola, A. Foudree, and S. R. Rodermel Chloroplast Photooxidation-Induced Transcriptome Reprogramming in Arabidopsis immutans White Leaf Sectors Plant Physiology, June 1, 2009; 150(2): 904 - 923. [Abstract] [Full Text] [PDF]

				M. Jubault, C. Hamon, A. Gravot, C. Lariagon, R. Delourme, A. Bouchereau, and M. J. Manzanares-Dauleux Differential Regulation of Root Arginine Catabolism and Polyamine Metabolism in Clubroot-Susceptible and Partially Resistant Arabidopsis Genotypes Plant Physiology, April 1, 2008; 146(4): 2008 - 2019. [Abstract] [Full Text] [PDF]

				S. Berger, A. K. Sinha, and T. Roitsch Plant physiology meets phytopathology: plant primary metabolism and plant pathogen interactions J. Exp. Bot., December 1, 2007; 58(15-16): 4019 - 4026. [Abstract] [Full Text] [PDF]

				M. Efetova, J. Zeier, M. Riederer, C.-W. Lee, N. Stingl, M. Mueller, W. Hartung, R. Hedrich, and R. Deeken A Central Role of Abscisic Acid in Drought Stress Protection of Agrobacterium-Induced Tumors on Arabidopsis Plant Physiology, November 1, 2007; 145(3): 853 - 862. [Abstract] [Full Text] [PDF]

				N. A. Eckardt A Genomic Analysis of Tumor Development and Source-Sink Relationships in Agrobacterium-Induced Crown Gall Disease in Arabidopsis PLANT CELL, December 1, 2006; 18(12): 3350 - 3352. [Full Text] [PDF]