First published online February 10, 2006; 10.1105/tpc.105.038372
The Plant Cell 18:731-746 (2006)
© 2006 American Society of Plant Biologists
OPEN ACCESS ARTICLE
Arabidopsis PEN3/PDR8, an ATP Binding Cassette Transporter, Contributes to Nonhost Resistance to Inappropriate Pathogens That Enter by Direct Penetration[W],[OA]
Mónica Steina,
Jan Dittgenb,
Clara Sánchez-Rodríguezc,
Bi-Huei Houa,
Antonio Molinac,
Paul Schulze-Lefertb,
Volker Lipkad and
Shauna Somervillea,1
a Carnegie Institution, Department of Plant Biology, Stanford, California, 94305
b Max-Planck-Institute for Plant Breeding Research, Department of PlantMicrobe Interactions, D-50829 Köln, Germany
c Centro de Biotecnología y Genómica de Plantas, Departamento de Biotecnología, Universidad Politecnica Madrid, Escuela Tecnica Superior, Ingenieros Agrónomos, E-28040 Madrid, Spain
d Eberhard-Karls-Universität Tübingen, Plant Biochemistry, D-72076 Tübingen, Germany
1 To whom correspondence should be addressed. E-mail ssomerville{at}stanford.edu; fax 650-325-6857.
Arabidopsis thaliana is a host to the powdery mildew Erysiphe cichoracearum and nonhost to Blumeria graminis f. sp hordei, the powdery mildew pathogenic on barley (Hordeum vulgare). Screening for Arabidopsis mutants deficient in resistance to barley powdery mildew identified PENETRATION3 (PEN3). pen3 plants permitted both increased invasion into epidermal cells and initiation of hyphae by B. g. hordei, suggesting that PEN3 contributes to defenses at the cell wall and intracellularly. pen3 mutants were compromised in resistance to the necrotroph Plectosphaerella cucumerina and to two additional inappropriate biotrophs, pea powdery mildew (Erysiphe pisi) and potato late blight (Phytophthora infestans). Unexpectedly, pen3 mutants were resistant to E. cichoracearum. This resistance was salicylic aciddependent and correlated with chlorotic patches. Consistent with this observation, salicylic acid pathway genes were hyperinduced in pen3 relative to the wild type. The phenotypes conferred by pen3 result from the loss of function of PLEIOTROPIC DRUG RESISTANCE8 (PDR8), a highly expressed putative ATP binding cassette transporter. PEN3/PDR8 tagged with green fluorescent protein localized to the plasma membrane in uninfected cells. In infected leaves, the protein concentrated at infection sites. PEN3/PDR8 may be involved in exporting toxic materials to attempted invasion sites, and intracellular accumulation of these toxins in pen3 may secondarily activate the salicylic acid pathway.
This article has been cited by other articles:

|
 |

|
 |
 
W. Underwood and S. C. Somerville
Focal accumulation of defences at sites of fungal pathogen attack
J. Exp. Bot.,
August 13, 2008;
(2008)
ern205v1.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
C. Kwon, P. Bednarek, and P. Schulze-Lefert
Secretory Pathways in Plant Immune Responses
Plant Physiology,
August 1, 2008;
147(4):
1575 - 1583.
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
C. E. Elliott, Harjono, and B. J. Howlett
Mutation of a Gene in the Fungus Leptosphaeria maculans Allows Increased Frequency of Penetration of Stomatal Apertures of Arabidopsis thaliana
Mol Plant,
May 9, 2008;
(2008)
ssn014v1.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
Z. Zhang, A. Lenk, M. X. Andersson, T. Gjetting, C. Pedersen, M. E. Nielsen, M.-A. Newman, B.-H. Hou, S. C. Somerville, and H. Thordal-Christensen
A Lesion-Mimic Syntaxin Double Mutant in Arabidopsis Reveals Novel Complexity of Pathogen Defense Signaling
Mol Plant,
May 1, 2008;
1(3):
510 - 527.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
L. Westphal, D. Scheel, and S. Rosahl
The coi1-16 Mutant Harbors a Second Site Mutation Rendering PEN2 Nonfunctional
PLANT CELL,
April 1, 2008;
20(4):
824 - 826.
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
D. V. Badri, V. M. Loyola-Vargas, C. D. Broeckling, C. De-la-Pena, M. Jasinski, D. Santelia, E. Martinoia, L. W. Sumner, L. M. Banta, F. Stermitz, et al.
Altered Profile of Secondary Metabolites in the Root Exudates of Arabidopsis ATP-Binding Cassette Transporter Mutants
Plant Physiology,
February 1, 2008;
146(2):
762 - 771.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
V. Cheng, H. U. Stotz, K. Hippchen, and A. T. Bakalinsky
Genome-Wide Screen for Oxalate-Sensitive Mutants of Saccharomyces cerevisiae
Appl. Envir. Microbiol.,
September 15, 2007;
73(18):
5919 - 5927.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
J. J. Benschop, S. Mohammed, M. O'Flaherty, A. J. R. Heck, M. Slijper, and F. L. H. Menke
Quantitative Phosphoproteomics of Early Elicitor Signaling in Arabidopsis
Mol. Cell. Proteomics,
July 1, 2007;
6(7):
1198 - 1214.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
D. Tang, M. T. Simonich, and R. W. Innes
Mutations in LACS2, a Long-Chain Acyl-Coenzyme A Synthetase, Enhance Susceptibility to Avirulent Pseudomonas syringae But Confer Resistance to Botrytis cinerea in Arabidopsis
Plant Physiology,
June 1, 2007;
144(2):
1093 - 1103.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
C. Hernandez-Blanco, D. X. Feng, J. Hu, A. Sanchez-Vallet, L. Deslandes, F. Llorente, M. Berrocal-Lobo, H. Keller, X. Barlet, C. Sanchez-Rodriguez, et al.
Impairment of Cellulose Synthases Required for Arabidopsis Secondary Cell Wall Formation Enhances Disease Resistance
PLANT CELL,
March 1, 2007;
19(3):
890 - 903.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
G. Liu, D. L. Greenshields, R. Sammynaiken, R. N. Hirji, G. Selvaraj, and Y. Wei
Targeted alterations in iron homeostasis underlie plant defense responses
J. Cell Sci.,
February 15, 2007;
120(4):
596 - 605.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
H. Ito and W. M. Gray
A Gain-of-Function Mutation in the Arabidopsis Pleiotropic Drug Resistance Transporter PDR9 Confers Resistance to Auxinic Herbicides
Plant Physiology,
September 1, 2006;
142(1):
63 - 74.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
J. Ellis
Insights into nonhost disease resistance: can they assist disease control in agriculture?
PLANT CELL,
March 1, 2006;
18(3):
523 - 528.
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
A. Sugiyama, N. Shitan, S. Sato, Y. Nakamura, S. Tabata, and K. Yazaki
Genome-wide analysis of ATP-binding cassette (ABC) proteins in a model legume plant, Lotus japonicus: comparison with Arabidopsis ABC protein family
DNA Res,
January 1, 2006;
13(5):
205 - 228.
[Abstract]
[Full Text]
[PDF]
|
 |
|
|
|