Plant Cell
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online April 28, 2006; 10.1105/tpc.106.041582

The Plant Cell 18:1438-1453 (2006)
© 2006 American Society of Plant Biologists

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
18/6/1438    most recent
tpc.106.041582v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (12)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dowd, P. E.
Right arrow Articles by Gilroy, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dowd, P. E.
Right arrow Articles by Gilroy, S.
Agricola
Right arrow Articles by Dowd, P. E.
Right arrow Articles by Gilroy, S.

Petunia Phospholipase C1 Is Involved in Pollen Tube Growth[W]

Peter E. Dowda, Sylvie Coursolb,1, Andrea L. Skirpana,2, Teh-hui Kaoa and Simon Gilroyb,3

a Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802
b Department of Biology, Pennsylvania State University, University Park, Pennsylvania 16802

3 To whom correspondence should be addressed. E-mail sxg12{at}psu.edu; fax 814-865-9131.

Although pollen tube growth is essential for plant fertilization and reproductive success, the regulators of the actin-related growth machinery and the cytosolic Ca2+ gradient thought to determine how these cells elongate remain poorly defined. Phospholipases, their substrates, and their phospholipid turnover products have been proposed as such regulators; however, the relevant phospholipase(s) have not been characterized. Therefore, we cloned cDNA for a pollen-expressed phosphatidylinositol 4,5-bisphosphate (PtdInsP2)–cleaving phospholipase C (PLC) from Petunia inflata, named Pet PLC1. Expressing a catalytically inactive form of Pet PLC1 in pollen tubes caused expansion of the apical Ca2+ gradient, disruption of the organization of the actin cytoskeleton, and delocalization of growth at the tube tip. These phenotypes were suppressed by depolymerizing actin with low concentrations of latrunculin B, suggesting that a critical site of action of Pet PLC1 is in regulating actin structure at the growing tip. A green fluorescent protein (GFP) fusion to Pet PLC1 caused enrichment in regions of the apical plasma membrane not undergoing rapid expansion, whereas a GFP fusion to the PtdInsP2 binding domain of mammalian PLC {delta}1 caused enrichment in apical regions depleted in PLC. Thus, Pet PLC1 appears to be involved in the machinery that restricts growth to the very apex of the elongating pollen tube, likely through its regulatory action on PtdInsP2 distribution within the cell.




This article has been cited by other articles:


Home page
 J Exp BotHome page
L. Li, N. Saga, and K. Mikami
Phosphatidylinositol 3-kinase activity and asymmetrical accumulation of F-actin are necessary for establishment of cell polarity in the early development of monospores from the marine red alga Porphyra yezoensis
J. Exp. Bot., August 13, 2008; (2008) ern207v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Yalovsky, D. Bloch, N. Sorek, and B. Kost
Regulation of Membrane Trafficking, Cytoskeleton Dynamics, and Cell Polarity by ROP/RAC GTPases
Plant Physiology, August 1, 2008; 147(4): 1527 - 1543.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
H.-J. Wang, A.-R. Wan, and G.-Y. Jauh
An Actin-Binding Protein, LlLIM1, Mediates Calcium and Hydrogen Regulation of Actin Dynamics in Pollen Tubes
Plant Physiology, August 1, 2008; 147(4): 1619 - 1636.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C.-M. Yoo, J. Wen, C. M. Motes, J. A. Sparks, and E. B. Blancaflor
A Class I ADP-Ribosylation Factor GTPase-Activating Protein Is Critical for Maintaining Directional Root Hair Growth in Arabidopsis
Plant Physiology, August 1, 2008; 147(4): 1659 - 1674.
[Abstract] [Full Text] [PDF]

Home page
 Mol PlantHome page
W.-Q. Yang, Y. Lai, M.-N. Li, W.-Y. Xu, and Y.-B. Xue
A Novel C2-Domain Phospholipid-Binding Protein, OsPBP1, Is Required for Pollen Fertility in Rice
Mol Plant, July 21, 2008; (2008) ssn035v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
L. Cardenas, A. Lovy-Wheeler, J. G. Kunkel, and P. K. Hepler
Pollen Tube Growth Oscillations and Intracellular Calcium Levels Are Reversibly Modulated by Actin Polymerization
Plant Physiology, April 1, 2008; 146(4): 1611 - 1621.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. M. Thole, J. E.M. Vermeer, Y. Zhang, T. W.J. Gadella Jr., and E. Nielsen
ROOT HAIR DEFECTIVE4 Encodes a Phosphatidylinositol-4-Phosphate Phosphatase Required for Proper Root Hair Development in Arabidopsis thaliana
PLANT CELL, February 1, 2008; 20(2): 381 - 395.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
H. Kusano, C. Testerink, J. E.M. Vermeer, T. Tsuge, H. Shimada, A. Oka, T. Munnik, and T. Aoyama
The Arabidopsis Phosphatidylinositol Phosphate 5-Kinase PIP5K3 Is a Key Regulator of Root Hair Tip Growth
PLANT CELL, February 1, 2008; 20(2): 367 - 380.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
I. Stenzel, T. Ischebeck, S. Konig, A. Holubowska, M. Sporysz, B. Hause, and I. Heilmann
The Type B Phosphatidylinositol-4-Phosphate 5-Kinase 3 Is Essential for Root Hair Formation in Arabidopsis thaliana
PLANT CELL, January 1, 2008; 20(1): 124 - 141.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Z. Hua, X. Meng, and T.-h. Kao
Comparison of Petunia inflata S-Locus F-Box Protein (Pi SLF) with Pi SLF Like Proteins Reveals Its Unique Function in S-RNase Based Self-Incompatibility
PLANT CELL, November 1, 2007; 19(11): 3593 - 3609.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Y. Xiang, X. Huang, T. Wang, Y. Zhang, Q. Liu, P. J. Hussey, and H. Ren
ACTIN BINDING PROTEIN29 from Lilium Pollen Plays an Important Role in Dynamic Actin Remodeling
PLANT CELL, June 1, 2007; 19(6): 1930 - 1946.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. J. Davis, Y. J. Im, J. S. Dubin, K. B. Tomer, and W. F. Boss
Arabidopsis Phosphatidylinositol Phosphate Kinase 1 Binds F-actin and Recruits Phosphatidylinositol 4-Kinase beta1 to the Actin Cytoskeleton
J. Biol. Chem., May 11, 2007; 282(19): 14121 - 14131.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Y. J. Im, I. Y. Perera, I. Brglez, A. J. Davis, J. Stevenson-Paulik, B. Q. Phillippy, E. Johannes, N. S. Allen, and W. F. Boss
Increasing Plasma Membrane Phosphatidylinositol(4,5)Bisphosphate Biosynthesis Increases Phosphoinositide Metabolism in Nicotiana tabacum
PLANT CELL, May 1, 2007; 19(5): 1603 - 1616.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. A. Khan, Q. Wang, R. D. Sjolund, A. Schulz, and G. A. Thompson
An Early Nodulin-Like Protein Accumulates in the Sieve Element Plasma Membrane of Arabidopsis
Plant Physiology, April 1, 2007; 143(4): 1576 - 1589.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
D. Helling, A. Possart, S. Cottier, U. Klahre, and B. Kost
Pollen Tube Tip Growth Depends on Plasma Membrane Polarization Mediated by Tobacco PLC3 Activity and Endocytic Membrane Recycling
PLANT CELL, December 1, 2006; 18(12): 3519 - 3534.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2006 by the American Society of Plant Biologists