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First published online May 5, 2006; 10.1105/tpc.106.041640

The Plant Cell 18:1498-1509 (2006)
© 2006 American Society of Plant Biologists

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The Arabidopsis Major Intrinsic Protein NIP5;1 Is Essential for Efficient Boron Uptake and Plant Development under Boron Limitation

Junpei Takanoa,1, Motoko Wadaa,1,2, Uwe Ludewigb, Gabriel Schaafc,3, Nicolaus von Wirénc and Toru Fujiwaraa,d,4

a Biotechnology Research Center, University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
b Zentrum für Molekularbiologie der Pflanzen, Pflanzenphysiologie, Universität Tübingen, 72076 Tübingen, Germany
c Institut für Pflanzenernährung, Universität Hohenheim, D-70593 Stuttgart, Germany
d Solution Oriented Research for Science and Technology, Japan Science and Technology Agency, Chuo-Ku, Tokyo 103-0027, Japan

4 To whom correspondence should be addressed. E-mail atorufu{at}mail.ecc.u-tokyo.ac.jp; fax 81-3-5841-2408.

Boron (B) is essential in plants but often present at low concentrations in the environment. To investigate how plants survive under conditions of B limitation, we conducted a transcriptome analysis and identified NIP5;1, a member of the major intrinsic protein family, as a gene upregulated in B-deficient roots of Arabidopsis thaliana. Promoter–ß-glucuronidase fusions indicated that NIP5;1 is strongly upregulated in the root elongation zone and the root hair zone under B limitation, and green fluorescent protein–tagged NIP5;1 proteins localized to the plasma membrane. Expression in Xenopus laevis oocytes demonstrated that NIP5;1 facilitated the transport of boric acid in addition to water. Importantly, two T-DNA insertion lines of NIP5;1 displayed lower boric acid uptake into roots, lower biomass production, and increased sensitivity of root and shoot development to B deficiency. These results identify NIP5;1 as a major plasma membrane boric acid channel crucial for the B uptake required for plant growth and development under B limitation.




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