First published online June 2, 2006; 10.1105/tpc.106.042184
The Plant Cell 18:1736-1749 (2006)
© 2006 American Society of Plant Biologists
STABILIZED1, a Stress-Upregulated Nuclear Protein, Is Required for Pre-mRNA Splicing, mRNA Turnover, and Stress Tolerance in Arabidopsis[W]
Byeong-ha Leea,1,
Avnish Kapoorb,
Jianhua Zhub and
Jian-Kang Zhua,b,2
a Department of Plant Sciences, University of Arizona, Tucson, Arizona 85721
b Institute for Integrative Genome Biology and Department of Botany and Plant Sciences, University of California, Riverside, California 92521
2 To whom correspondence should be addressed. E-mail jian-kang.zhu{at}ucr.edu; fax 951-827-7115.
In plants, many gene transcripts are very unstable, which is important for the tight control of their temporal and spatial expression patterns. To identify cellular factors controlling the stability of unstable mRNAs in plants, we used luciferase imaging in Arabidopsis thaliana to isolate a recessive mutant, stabilized1-1 (sta1-1), with enhanced stability of the normally unstable luciferase transcript. The sta1-1 mutation also causes the stabilization of some endogenous gene transcripts and has a range of developmental and stress response phenotypes. STA1 encodes a nuclear protein similar to the human U5 small ribonucleoprotein–associated 102-kD protein and to the yeast pre-mRNA splicing factors Prp1p and Prp6p. STA1 expression is upregulated by cold stress, and the sta1-1 mutant is defective in the splicing of the cold-induced COR15A gene. Our results show that STA1 is a pre-mRNA splicing factor required not only for splicing but also for the turnover of unstable transcripts and that it has an important role in plant responses to abiotic stresses.
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