This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 18/7/1736    most recent tpc.106.042184v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (13) Citing Articles via Google Scholar Google Scholar Articles by Lee, B.-h. Articles by Zhu, J.-K. Search for Related Content PubMed PubMed Citation Articles by Lee, B.-h. Articles by Zhu, J.-K. Agricola Articles by Lee, B.-h. Articles by Zhu, J.-K.

STABILIZED1, a Stress-Upregulated Nuclear Protein, Is Required for Pre-mRNA Splicing, mRNA Turnover, and Stress Tolerance in Arabidopsis^[W]

^a Department of Plant Sciences, University of Arizona, Tucson, Arizona 85721
^b Institute for Integrative Genome Biology and Department of Botany and Plant Sciences, University of California, Riverside, California 92521

² To whom correspondence should be addressed. E-mail jian-kang.zhu{at}ucr.edu; fax 951-827-7115.

In plants, many gene transcripts are very unstable, which is important for the tight control of their temporal and spatial expression patterns. To identify cellular factors controlling the stability of unstable mRNAs in plants, we used luciferase imaging in Arabidopsis thaliana to isolate a recessive mutant, stabilized1-1 (sta1-1), with enhanced stability of the normally unstable luciferase transcript. The sta1-1 mutation also causes the stabilization of some endogenous gene transcripts and has a range of developmental and stress response phenotypes. STA1 encodes a nuclear protein similar to the human U5 small ribonucleoprotein–associated 102-kD protein and to the yeast pre-mRNA splicing factors Prp1p and Prp6p. STA1 expression is upregulated by cold stress, and the sta1-1 mutant is defective in the splicing of the cold-induced COR15A gene. Our results show that STA1 is a pre-mRNA splicing factor required not only for splicing but also for the turnover of unstable transcripts and that it has an important role in plant responses to abiotic stresses.

This article has been cited by other articles:

				A. Wasilewska, F. Vlad, C. Sirichandra, Y. Redko, F. Jammes, C. Valon, N. F. d. Frey, and J. Leung An Update on Abscisic Acid Signaling in Plants and More ... Mol Plant, March 1, 2008; 1(2): 198 - 217. [Abstract] [Full Text] [PDF]

				J. Sun, H. Jiang, Y. Xu, H. Li, X. Wu, Q. Xie, and C. Li The CCCH-Type Zinc Finger Proteins AtSZF1 and AtSZF2 Regulate Salt Stress Responses in Arabidopsis Plant Cell Physiol., August 1, 2007; 48(8): 1148 - 1158. [Abstract] [Full Text] [PDF]

				I. Heiber, E. Stroher, B. Raatz, I. Busse, U. Kahmann, M. W. Bevan, K.-J. Dietz, and M. Baier The redox imbalanced Mutants of Arabidopsis Differentiate Signaling Pathways for Redox Regulation of Chloroplast Antioxidant Enzymes Plant Physiology, April 1, 2007; 143(4): 1774 - 1788. [Abstract] [Full Text] [PDF]

				W. Bang, S. Kim, A. Ueda, M. Vikram, D. Yun, R. A. Bressan, P. M. Hasegawa, J. Bahk, and H. Koiwa Arabidopsis Carboxyl-Terminal Domain Phosphatase-Like Isoforms Share Common Catalytic and Interaction Domains But Have Distinct in Planta Functions Plant Physiology, October 1, 2006; 142(2): 586 - 594. [Abstract] [Full Text] [PDF]