Plant Cell Hybrigenics The Protein Interactions Experts
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online June 23, 2006; 10.1105/tpc.106.041749

The Plant Cell 18:1908-1930 (2006)
© 2006 American Society of Plant Biologists

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
18/8/1908    most recent
tpc.106.041749v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (10)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schmidt, M.
Right arrow Articles by Kreimer, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schmidt, M.
Right arrow Articles by Kreimer, G.
Agricola
Right arrow Articles by Schmidt, M.
Right arrow Articles by Kreimer, G.

Proteomic Analysis of the Eyespot of Chlamydomonas reinhardtii Provides Novel Insights into Its Components and Tactic Movements[W]

Melanie Schmidta,1, Gunther Geßnerb,1, Matthias Luffa,1, Ines Heilandb, Volker Wagnerb, Marc Kaminskib, Stefan Geimerc, Nicole Eitzingera, Tobias Reißenwebera, Olga Voytsekhb, Monika Fiedlerb, Maria Mittagb and Georg Kreimera,2

a Institute of Biology, Friedrich-Alexander-University, D-91058 Erlangen, Germany
b Institute of General Botany and Plant Physiology, Friedrich-Schiller-University, D-07743 Jena, Germany
c Cell Biology/Electron Microscopy, University of Bayreuth, D-95440 Bayreuth, Germany

2 To whom correspondence should be addressed. E-mail gkreimer{at}biologie.uni-erlangen.de; fax 49-09131-8528215.

Flagellate green algae have developed a visual system, the eyespot apparatus, which allows the cell to phototax. To further understand the molecular organization of the eyespot apparatus and the phototactic movement that is controlled by light and the circadian clock, a detailed understanding of all components of the eyespot apparatus is needed. We developed a procedure to purify the eyespot apparatus from the green model alga Chlamydomonas reinhardtii. Its proteomic analysis resulted in the identification of 202 different proteins with at least two different peptides (984 in total). These data provide new insights into structural components of the eyespot apparatus, photoreceptors, retina(l)-related proteins, members of putative signaling pathways for phototaxis and chemotaxis, and metabolic pathways within an algal visual system. In addition, we have performed a functional analysis of one of the identified putative components of the phototactic signaling pathway, casein kinase 1 (CK1). CK1 is also present in the flagella and thus is a promising candidate for controlling behavioral responses to light. We demonstrate that silencing CK1 by RNA interference reduces its level in both flagella and eyespot. In addition, we show that silencing of CK1 results in severe disturbances in hatching, flagellum formation, and circadian control of phototaxis.




This article has been cited by other articles:


Home page
 Eukaryot CellHome page
E. R. Moellering and C. Benning
RNA Interference Silencing of a Major Lipid Droplet Protein Affects Lipid Droplet Size in Chlamydomonas reinhardtii
Eukaryot. Cell, January 1, 2010; 9(1): 97 - 106.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
A. Gokhale, M. Wirschell, and W. S. Sale
Regulation of dynein-driven microtubule sliding by the axonemal protein kinase CK1 in Chlamydomonas flagella
J. Cell Biol., September 21, 2009; 186(6): 817 - 824.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Kianianmomeni, K. Stehfest, G. Nematollahi, P. Hegemann, and A. Hallmann
Channelrhodopsins of Volvox carteri Are Photochromic Proteins That Are Specifically Expressed in Somatic Cells under Control of Light, Temperature, and the Sex Inducer
Plant Physiology, September 1, 2009; 151(1): 347 - 366.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S.-i. Ozawa, J. Nield, A. Terao, E. J. Stauber, M. Hippler, H. Koike, J.-D. Rochaix, and Y. Takahashi
Biochemical and Structural Studies of the Large Ycf4-Photosystem I Assembly Complex of the Green Alga Chlamydomonas reinhardtii
PLANT CELL, August 1, 2009; 21(8): 2424 - 2442.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
J. Boesger, V. Wagner, W. Weisheit, and M. Mittag
Analysis of Flagellar Phosphoproteins from Chlamydomonas reinhardtii
Eukaryot. Cell, July 1, 2009; 8(7): 922 - 932.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
T. M. Mittelmeier, P. Berthold, A. Danon, M. R. Lamb, A. Levitan, M. E. Rice, and C. L. Dieckmann
C2 Domain Protein MIN1 Promotes Eyespot Organization in Chlamydomonas reinhardtii
Eukaryot. Cell, December 1, 2008; 7(12): 2100 - 2112.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
O. Voytsekh, S. B. Seitz, D. Iliev, and M. Mittag
Both Subunits of the Circadian RNA-Binding Protein CHLAMY1 Can Integrate Temperature Information
Plant Physiology, August 1, 2008; 147(4): 2179 - 2193.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
P. Berthold, S. P. Tsunoda, O. P. Ernst, W. Mages, D. Gradmann, and P. Hegemann
Channelrhodopsin-1 Initiates Phototaxis and Photophobic Responses in Chlamydomonas by Immediate Light-Induced Depolarization
PLANT CELL, June 1, 2008; 20(6): 1665 - 1677.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
O. Vallon and S. Dutcher
Treasure Hunting in the Chlamydomonas Genome
Genetics, May 1, 2008; 179(1): 3 - 6.
[Full Text] [PDF]

Home page
 Genes Dev.Home page
M. Brunner and M. Merrow
The green yeast uses its plant-like clock to regulate its animal-like tail
Genes & Dev., April 1, 2008; 22(7): 825 - 831.
[Full Text] [PDF]

Home page
 Genes Dev.Home page
T. Matsuo, K. Okamoto, K. Onai, Y. Niwa, K. Shimogawara, and M. Ishiura
A systematic forward genetic analysis identified components of the Chlamydomonas circadian system
Genes & Dev., April 1, 2008; 22(7): 918 - 930.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
V. Wagner, K. Ullmann, A. Mollwo, M. Kaminski, M. Mittag, and G. Kreimer
The Phosphoproteome of a Chlamydomonas reinhardtii Eyespot Fraction Includes Key Proteins of the Light Signaling Pathway
Plant Physiology, February 1, 2008; 146(2): 772 - 788.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
S. S. Merchant, S. E. Prochnik, O. Vallon, E. H. Harris, S. J. Karpowicz, G. B. Witman, A. Terry, A. Salamov, L. K. Fritz-Laylin, L. Marechal-Drouard, et al.
The Chlamydomonas Genome Reveals the Evolution of Key Animal and Plant Functions
Science, October 12, 2007; 318(5848): 245 - 250.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. Willmund, T. Muhlhaus, M. Wojciechowska, and M. Schroda
The NH2-terminal Domain of the Chloroplast GrpE Homolog CGE1 Is Required for Dimerization and Cochaperone Function in Vivo
J. Biol. Chem., April 13, 2007; 282(15): 11317 - 11328.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. Iliev, O. Voytsekh, E.-M. Schmidt, M. Fiedler, A. Nykytenko, and M. Mittag
A Heteromeric RNA-Binding Protein Is Involved in Maintaining Acrophase and Period of the Circadian Clock
Plant Physiology, October 1, 2006; 142(2): 797 - 806.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2006 by the American Society of Plant Biologists