First published online August 4, 2006; 10.1105/tpc.106.041541
The Plant Cell 18:2356-2368 (2006)
© 2006 American Society of Plant Biologists
Rice NTRC Is a High-Efficiency Redox System for Chloroplast Protection against Oxidative Damage[W]
Juan Manuel Pérez-Ruiza,
María Cristina Spínolaa,
Kerstin Kirchsteigera,
Javier Morenob,
Mariam Sahrawyc and
Francisco Javier Cejudoa,1
a Instituto de Bioquímica Vegetal y Fotosíntesis, Centro de Investigaciones Científicas Isla de la Cartuja, 41092 Seville, Spain
b Departamento de Biología Celular, Facultad de Biología, Universidad de Sevilla, 41013 Seville, Spain
c Departamento de Bioquímica y Biología Molecular de Plantas, Estación Experimental del Zaidín, 18008 Granada, Spain
1 To whom correspondence should be addressed. E-mail fjcejudo{at}us.es; fax 34-954460-0065.
One of the mechanisms plants have developed for chloroplast protection against oxidative damage involves a 2-Cys peroxiredoxin, which has been proposed to be reduced by ferredoxin and plastid thioredoxins, Trx x and CDSP32, the FTR/Trx pathway. We show that rice (Oryza sativa) chloroplast NADPH THIOREDOXIN REDUCTASE (NTRC), with a thioredoxin domain, uses NADPH to reduce the chloroplast 2-Cys peroxiredoxin BAS1, which then reduces hydrogen peroxide. The presence of both NTR and Trx-like domains in a single polypeptide is absolutely required for the high catalytic efficiency of NTRC. An Arabidopsis thaliana knockout mutant for NTRC shows irregular mesophyll cell shape, abnormal chloroplast structure, and unbalanced BAS1 redox state, resulting in impaired photosynthesis rate under low light. Constitutive expression of wild-type NTRC in mutant transgenic lines rescued this phenotype. Moreover, prolonged darkness followed by light/dark incubation produced an increase in hydrogen peroxide and lipid peroxidation in leaves and accelerated senescence of NTRC-deficient plants. We propose that NTRC constitutes an alternative system for chloroplast protection against oxidative damage, using NADPH as the source of reducing power. Since no light-driven reduced ferredoxin is produced at night, the NTRC-BAS1 pathway may be a key detoxification system during darkness, with NADPH produced by the oxidative pentose phosphate pathway as the source of reducing power.
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