OPEN ACCESS ARTICLE

This Article Free via Open Access: OA OA Full Text Full Text (PDF) Supplemental Data OA All Versions of this Article: 19/1/270    most recent tpc.106.047043v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (28) Citing Articles via Google Scholar Google Scholar Articles by Mitsuda, N. Articles by Ohme-Takagi, M. Search for Related Content PubMed PubMed Citation Articles by Mitsuda, N. Articles by Ohme-Takagi, M. Agricola Articles by Mitsuda, N. Articles by Ohme-Takagi, M.

NAC Transcription Factors, NST1 and NST3, Are Key Regulators of the Formation of Secondary Walls in Woody Tissues of Arabidopsis^[W],[OA]

^a Research Institute of Genome-Based Biofactory, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8562, Japan
^b Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012, Japan
^c Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan
^d Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, Tsukuba 305-0074, Japan
^e RIKEN Plant Science Center, RIKEN Yokohama Institute, Tsurumi, Yokohama 230-0045, Japan

¹ To whom correspondence should be addressed. E-mail m-takagi{at}aist.go.jp; fax 81-29-861-3026.

Wood is formed by the successive addition of secondary xylem, which consists of cells with a conspicuously thickened secondary wall composed mainly of lignin and cellulose. Several genes involved in lignin and cellulose biosynthesis have been characterized, but the factors that regulate the formation of secondary walls in woody tissues remain to be identified. In this study, we show that plant-specific transcription factors, designated NAC SECONDARY WALL THICKENING PROMOTING FACTOR1 (NST1) and NST3, are key regulators of the formation of secondary walls in woody tissues of Arabidopsis thaliana. In nst1-1 nst3-1 double knockout plants, the secondary wall thickenings in interfascicular fibers and secondary xylem, except for vascular vessels, were completely suppressed without affecting formation of cells destined to be woody tissues. Conversely, as shown previously for NST1, overexpression of NST3 induced ectopic secondary wall thickenings in various aboveground tissues. Furthermore, the expression of chimeric repressors derived from NST1 and NST3 suppressed secondary wall thickenings in the presumptive interfascicular fibers. Because putative orthologs of NST1 and NST3 are present in the genome of poplar, our results suggest that they are also key regulators of the formation of secondary walls in woody plants and could be used as a tool for the genetic engineering of wood and its derivatives.

This article has been cited by other articles:

				R. Zhong, C. Lee, and Z.-H. Ye Functional Characterization of Poplar Wood-Associated NAC Domain Transcription Factors Plant Physiology, February 1, 2010; 152(2): 1044 - 1055. [Abstract] [Full Text] [PDF]

				M. Shikata, T. Koyama, N. Mitsuda, and M. Ohme-Takagi Arabidopsis SBP-Box Genes SPL10, SPL11 and SPL2 Control Morphological Change in Association with Shoot Maturation in the Reproductive Phase Plant Cell Physiol., December 1, 2009; 50(12): 2133 - 2145. [Abstract] [Full Text] [PDF]

				R. L. McCarthy, R. Zhong, and Z.-H. Ye MYB83 Is a Direct Target of SND1 and Acts Redundantly with MYB46 in the Regulation of Secondary Cell Wall Biosynthesis in Arabidopsis Plant Cell Physiol., November 1, 2009; 50(11): 1950 - 1964. [Abstract] [Full Text] [PDF]

				T. Aohara, T. Kotake, Y. Kaneko, H. Takatsuji, Y. Tsumuraya, and S. Kawasaki Rice BRITTLE CULM 5 (BRITTLE NODE) is Involved in Secondary Cell Wall Formation in the Sclerenchyma Tissue of Nodes Plant Cell Physiol., November 1, 2009; 50(11): 1886 - 1897. [Abstract] [Full Text] [PDF]

				Y. Guo, G. Qin, H. Gu, and L.-J. Qu Dof5.6/HCA2, a Dof Transcription Factor Gene, Regulates Interfascicular Cambium Formation and Vascular Tissue Development in Arabidopsis PLANT CELL, November 1, 2009; 21(11): 3518 - 3534. [Abstract] [Full Text] [PDF]

				J. Zhou, C. Lee, R. Zhong, and Z.-H. Ye MYB58 and MYB63 Are Transcriptional Activators of the Lignin Biosynthetic Pathway during Secondary Cell Wall Formation in Arabidopsis PLANT CELL, January 1, 2009; 21(1): 248 - 266. [Abstract] [Full Text] [PDF]

				C. Bomal, F. Bedon, S. Caron, S. D. Mansfield, C. Levasseur, J. E. K. Cooke, S. Blais, L. Tremblay, M.-J. Morency, N. Pavy, et al. Involvement of Pinus taeda MYB1 and MYB8 in phenylpropanoid metabolism and secondary cell wall biogenesis: a comparative in planta analysis J. Exp. Bot., October 1, 2008; 59(14): 3925 - 3939. [Abstract] [Full Text] [PDF]

				R. Zhong, C. Lee, J. Zhou, R. L. McCarthy, and Z.-H. Ye A Battery of Transcription Factors Involved in the Regulation of Secondary Cell Wall Biosynthesis in Arabidopsis PLANT CELL, October 1, 2008; 20(10): 2763 - 2782. [Abstract] [Full Text] [PDF]

				A. Heyl, E. Ramireddy, W. G. Brenner, M. Riefler, J. Allemeersch, and T. Schmulling The Transcriptional Repressor ARR1-SRDX Suppresses Pleiotropic Cytokinin Activities in Arabidopsis Plant Physiology, July 1, 2008; 147(3): 1380 - 1395. [Abstract] [Full Text] [PDF]

				C. Yang, G. Vizcay-Barrena, K. Conner, and Z. A. Wilson MALE STERILITY1 Is Required for Tapetal Development and Pollen Wall Biosynthesis PLANT CELL, November 1, 2007; 19(11): 3530 - 3548. [Abstract] [Full Text] [PDF]

				R. Zhong, E. A. Richardson, and Z.-H. Ye The MYB46 Transcription Factor Is a Direct Target of SND1 and Regulates Secondary Wall Biosynthesis in Arabidopsis PLANT CELL, September 1, 2007; 19(9): 2776 - 2792. [Abstract] [Full Text] [PDF]