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First published online October 19, 2007; 10.1105/tpc.107.054361

The Plant Cell 19:3100-3110 (2007)
© 2007 American Society of Plant Biologists

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MIDGET Unravels Functions of the Arabidopsis Topoisomerase VI Complex in DNA Endoreduplication, Chromatin Condensation, and Transcriptional Silencing[W]

Viktor Kirik1,2, Andrea Schrader, Joachim F. Uhrig and Martin Hulskamp

University of Cologne, Botanical Institute III, 50931 Cologne, Germany

2 Address correspondence to vkirik{at}stanford.edu.

The plant homologs of the archaeal DNA topoisomerase VI complex are required for the progression of endoreduplication cycles. Here, we describe the identification of MIDGET (MID) as a novel component of topoisomerase VI. We show that mid mutants show the same phenotype as rhl1, rhl2, and top6B mutants and that MID protein physically interacts with RHL1. The phenotypic analysis revealed new phenotypes, indicating that topoisomerase VI is involved in chromatin organization and transcriptional silencing. In addition, genetic evidence is provided suggesting that the ATR-dependent DNA damage repair checkpoint is activated in mid mutants, and CYCB1;1 is ectopically activated. Finally, we demonstrate that overexpression of CYCB1;2 can rescue the endoreduplication defects in mid mutants, suggesting that in mid mutants, a specific checkpoint is activated preventing further progression of endoreduplication cycles.


Related articles in Plant Cell:

MIDGET and the Function of Topoisomerase VI in Arabidopsis
Nancy A. Eckardt
Plant Cell 2007 19: 2970. [Full Text]  





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