This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 19/10/3111    most recent tpc.107.052621v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via ISI Web of Science (5) Citing Articles via Google Scholar Google Scholar Articles by Kandasamy, M. K. Articles by Meagher, R. B. Search for Related Content PubMed PubMed Citation Articles by Kandasamy, M. K. Articles by Meagher, R. B. Agricola Articles by Kandasamy, M. K. Articles by Meagher, R. B.

Class-Specific Interaction of Profilin and ADF Isovariants with Actin in the Regulation of Plant Development^[W]

Department of Genetics, Davison Life Sciences Building, University of Georgia, Athens, Georgia 30602

¹ Address correspondence to meagher{at}uga.edu.

Two ancient and highly divergent actin-based cytoskeletal systems have evolved in angiosperms. Plant genomes encode complex actin and actin binding protein (ABP) gene families, most of which are phylogenetically grouped into gene classes with distinct vegetative or constitutive and reproductive expression patterns. In Arabidopsis thaliana, ectopic expression of high levels of a reproductive class actin, ACT1, in vegetative tissues causes severe dwarfing of plants with aberrant organization of most plant organs and cell types due to a severely altered actin cytoskeletal architecture. Overexpression of the vegetative class actin ACT2 to similar levels, however, produces insignificant phenotypic changes. We proposed that the misexpression of the pollen-specific ACT1 in vegetative cell types affects the dynamics of actin due to its inappropriate interaction with endogenous vegetative ABPs. To examine the functionally distinct interactions among the major classes of actins and ABPs, we ectopically coexpressed reproductive profilin (PRF4) or actin-depolymerizing factor (ADF) isovariants (e.g., ADF7) with ACT1. Our results demonstrated that the coexpression of these reproductive, but not vegetative, ABP isovariants suppressed the ectopic ACT1 expression phenotypes and restored wild-type stature and normal actin cytoskeletal architecture to the double transgenic plants. Thus, the actins and ABPs appear to have evolved class-specific, protein–protein interactions that are essential to the normal regulation of plant growth and development.

This article has been cited by other articles:

				M. K. Kandasamy, E. C. McKinney, and R. B. Meagher A Single Vegetative Actin Isovariant Overexpressed under the Control of Multiple Regulatory Sequences Is Sufficient for Normal Arabidopsis Development PLANT CELL, March 1, 2009; 21(3): 701 - 718. [Abstract] [Full Text] [PDF]

				C. Zhang, E. L. Mallery, J. Schlueter, S. Huang, Y. Fan, S. Brankle, C. J. Staiger, and D. B. Szymanski Arabidopsis SCARs Function Interchangeably to Meet Actin-Related Protein 2/3 Activation Thresholds during Morphogenesis PLANT CELL, April 1, 2008; 20(4): 995 - 1011. [Abstract] [Full Text] [PDF]

				T. Okamoto, S. Tsurumi, K. Shibasaki, Y. Obana, H. Takaji, Y. Oono, and A. Rahman Genetic Dissection of Hormonal Responses in the Roots of Arabidopsis Grown under Continuous Mechanical Impedance Plant Physiology, April 1, 2008; 146(4): 1651 - 1662. [Abstract] [Full Text] [PDF]