This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 19/10/3170    most recent tpc.107.050989v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (4) Citing Articles via Google Scholar Google Scholar Articles by Reumann, S. Articles by Jahn, O. Search for Related Content PubMed PubMed Citation Articles by Reumann, S. Articles by Jahn, O. Agricola Articles by Reumann, S. Articles by Jahn, O.

Proteome Analysis of Arabidopsis Leaf Peroxisomes Reveals Novel Targeting Peptides, Metabolic Pathways, and Defense Mechanisms^[W]

^a Department of Plant Biochemistry, Georg-August-University of Goettingen, Albrecht-von-Haller-Institute for Plant Sciences, D-37077 Goettingen, Germany
^b Max-Planck-Institute of Molecular Plant Physiology, Metabolic Networks, D-14424 Potsdam, Germany
^c University of Potsdam, Institute of Biochemistry and Biology, 14469 Potsdam, Germany
^d Max-Planck-Institute of Experimental Medicine, Proteomics Group, D-37075 Goettingen, Germany
^e Deutsche Forschungsgemeinschaft Research Center for Molecular Physiology of the Brain, D-37073 Goettingen, Germany

² Address correspondence to sreuman{at}gwdg.de.

We have established a protocol for the isolation of highly purified peroxisomes from mature Arabidopsis thaliana leaves and analyzed the proteome by complementary gel-based and gel-free approaches. Seventy-eight nonredundant proteins were identified, of which 42 novel proteins had previously not been associated with plant peroxisomes. Seventeen novel proteins carried predicted peroxisomal targeting signals (PTS) type 1 or type 2; 11 proteins contained PTS-related peptides. Peroxisome targeting was supported for many novel proteins by in silico analyses and confirmed for 11 representative full-length fusion proteins by fluorescence microscopy. The targeting function of predicted and unpredicted signals was investigated and SSL>, SSI>, and ASL> were established as novel functional PTS1 peptides. In contrast with the generally accepted confinement of PTS2 peptides to the N-terminal domain, the bifunctional transthyretin-like protein was demonstrated to carry internally a functional PTS2. The novel enzymes include numerous enoyl-CoA hydratases, short-chain dehydrogenases, and several enzymes involved in NADP and glutathione metabolism. Seven proteins, including ß-glucosidases and myrosinases, support the currently emerging evidence for an important role of leaf peroxisomes in defense against pathogens and herbivores. The data provide new insights into the biology of plant peroxisomes and improve the prediction accuracy of peroxisome-targeted proteins from genome sequences.

This article has been cited by other articles:

				T. Gabaldon Peroxisome diversity and evolution Phil Trans R Soc B, March 12, 2010; 365(1541): 765 - 773. [Abstract] [Full Text] [PDF]

				A. E. Agee, M. Surpin, E. J. Sohn, T. Girke, A. Rosado, B. W. Kram, C. Carter, A. M. Wentzell, D. J. Kliebenstein, H. C. Jin, et al. MODIFIED VACUOLE PHENOTYPE1 Is an Arabidopsis Myrosinase-Associated Protein Involved in Endomembrane Protein Trafficking Plant Physiology, January 1, 2010; 152(1): 120 - 132. [Abstract] [Full Text] [PDF]

				F. J. Corpas, M. Hayashi, S. Mano, M. Nishimura, and J. B. Barroso Peroxisomes Are Required for in Vivo Nitric Oxide Accumulation in the Cytosol following Salinity Stress of Arabidopsis Plants Plant Physiology, December 1, 2009; 151(4): 2083 - 2094. [Abstract] [Full Text] [PDF]

				J. Joyard, M. Ferro, C. Masselon, D. Seigneurin-Berny, D. Salvi, J. Garin, and N. Rolland Chloroplast Proteomics and the Compartmentation of Plastidial Isoprenoid Biosynthetic Pathways Mol Plant, November 12, 2009; (2009) ssp088v1. [Abstract] [Full Text] [PDF]

				U. Armbruster, A. Hertle, E. Makarenko, J. Zuhlke, M. Pribil, A. Dietzmann, I. Schliebner, E. Aseeva, E. Fenino, M. Scharfenberg, et al. Chloroplast Proteins without Cleavable Transit Peptides: Rare Exceptions or a Major Constituent of the Chloroplast Proteome? Mol Plant, November 1, 2009; 2(6): 1325 - 1335. [Abstract] [Full Text] [PDF]

				M. J. Lingard and B. Bartel Arabidopsis LON2 Is Necessary for Peroxisomal Function and Sustained Matrix Protein Import Plant Physiology, November 1, 2009; 151(3): 1354 - 1365. [Abstract] [Full Text] [PDF]

				P. K. Arthur, M. Claussen, S. Koch, K. Tarbashevich, O. Jahn, and T. Pieler Participation of Xenopus Elr-type Proteins in Vegetal mRNA Localization during Oogenesis J. Biol. Chem., July 24, 2009; 284(30): 19982 - 19992. [Abstract] [Full Text] [PDF]

				Y. Xiong, C. DeFraia, D. Williams, X. Zhang, and Z. Mou Characterization of Arabidopsis 6-Phosphogluconolactonase T-DNA Insertion Mutants Reveals an Essential Role for the Oxidative Section of the Plastidic Pentose Phosphate Pathway in Plant Growth and Development Plant Cell Physiol., July 1, 2009; 50(7): 1277 - 1291. [Abstract] [Full Text] [PDF]

				S. Reumann, S. Quan, K. Aung, P. Yang, K. Manandhar-Shrestha, D. Holbrook, N. Linka, R. Switzenberg, C. G. Wilkerson, A. P.M. Weber, et al. In-Depth Proteome Analysis of Arabidopsis Leaf Peroxisomes Combined with in Vivo Subcellular Targeting Verification Indicates Novel Metabolic and Regulatory Functions of Peroxisomes Plant Physiology, May 1, 2009; 150(1): 125 - 143. [Abstract] [Full Text] [PDF]

				N. Linka, F. L. Theodoulou, R. P. Haslam, M. Linka, J. A. Napier, H. E. Neuhaus, and A. P.M. Weber Peroxisomal ATP Import Is Essential for Seedling Development in Arabidopsis thaliana PLANT CELL, December 1, 2008; 20(12): 3241 - 3257. [Abstract] [Full Text] [PDF]

				H. Eubel, E. H. Meyer, N. L. Taylor, J. D. Bussell, N. O'Toole, J. L. Heazlewood, I. Castleden, I. D. Small, S. M. Smith, and A. H. Millar Novel Proteins, Putative Membrane Transporters, and an Integrated Metabolic Network Are Revealed by Quantitative Proteomic Analysis of Arabidopsis Cell Culture Peroxisomes Plant Physiology, December 1, 2008; 148(4): 1809 - 1829. [Abstract] [Full Text] [PDF]

				M. Sapir-Mir, A. Mett, E. Belausov, S. Tal-Meshulam, A. Frydman, D. Gidoni, and Y. Eyal Peroxisomal Localization of Arabidopsis Isopentenyl Diphosphate Isomerases Suggests That Part of the Plant Isoprenoid Mevalonic Acid Pathway Is Compartmentalized to Peroxisomes Plant Physiology, November 1, 2008; 148(3): 1219 - 1228. [Full Text] [PDF]

				C. Ma and S. Reumann Improved prediction of peroxisomal PTS1 proteins from genome sequences based on experimental subcellular targeting analyses as exemplified for protein kinases from Arabidopsis J. Exp. Bot., October 1, 2008; 59(13): 3767 - 3779. [Abstract] [Full Text] [PDF]

				B. K. Zolman, N. Martinez, A. Millius, A. R. Adham, and B. Bartel Identification and Characterization of Arabidopsis Indole-3-Butyric Acid Response Mutants Defective in Novel Peroxisomal Enzymes Genetics, September 1, 2008; 180(1): 237 - 251. [Abstract] [Full Text] [PDF]

				M. C. Lopez-Martin, M. Becana, L. C. Romero, and C. Gotor Knocking Out Cytosolic Cysteine Synthesis Compromises the Antioxidant Capacity of the Cytosol to Maintain Discrete Concentrations of Hydrogen Peroxide in Arabidopsis Plant Physiology, June 1, 2008; 147(2): 562 - 572. [Abstract] [Full Text] [PDF]

				Y. Arai, M. Hayashi, and M. Nishimura Proteomic Analysis of Highly Purified Peroxisomes from Etiolated Soybean Cotyledons Plant Cell Physiol., April 1, 2008; 49(4): 526 - 539. [Abstract] [Full Text] [PDF]