This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 19/11/3549    most recent tpc.107.054536v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (1) Citing Articles via Google Scholar Google Scholar Articles by Ito, T. Articles by Shinozaki, K. Search for Related Content PubMed PubMed Citation Articles by Ito, T. Articles by Shinozaki, K. Agricola Articles by Ito, T. Articles by Shinozaki, K.

Arabidopsis MALE STERILITY1 Encodes a PHD-Type Transcription Factor and Regulates Pollen and Tapetum Development^[W]

^a Antibiotics Laboratory, RIKEN, Tsukuba 305-0074, Japan
^b Department of Biology, Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, Pennsylvania 16802
^c Department of Chemical Biological Sciences, Faculty of Science, Japan Women's University, Bunkyo, Tokyo 112-8681, Japan
^d Central Research Laboratory, Advanced Research Laboratory, Hitachi, Hatoyama, Saitama 350-0395, Japan
^e Gene Regulation Research Group, Research Institute of Genome-Based Biofactory, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8566, Japan
^f Gene Discovery Research Group, RIKEN Plant Science Center, Yokohama 230-0045, Japan

¹ Address correspondence to hxm16{at}psu.edu or sinozaki{at}rtc.riken.jp.

The Arabidopsis thaliana MALE STERILITY1 (MS1) gene encodes a nuclear protein with Leu zipper–like and PHD-finger motifs and is important for postmeiotic pollen development. Here, we examined MS1 function using both cell biological and molecular biological approaches. We introduced a fusion construct of MS1 and a transcriptional repression domain (MS1-SRDX) into wild-type Arabidopsis, and the transgenic plants showed a semisterile phenotype similar to that of ms1. Since the repression domain can convert various kinds of transcriptional activators to dominant repressors, this suggested that MS1 functioned as a transcriptional activator. The Leu zipper–like region and the PHD motif were required for the MS1 function. Phenotypic analysis of the ms1 mutant and the MS1-SRDX transgenic Arabidopsis indicated that MS1 was involved in formation of pollen exine and pollen cytosolic components as well as tapetum development. Next, we searched for MS1 downstream genes by analyzing publicly available microarray data and identified 95 genes affected by MS1. Using a transgenic ms1 plant showing dexamethasone-inducible recovery of fertility, we further examined whether these genes were immediately downstream of MS1. From these results, we discuss a role of MS1 in pollen and tapetum development and the conservation of MS1 function in flowering plants.

This article has been cited by other articles:

				Y. Wang, H. Wu, and M. Yang Microscopy and Bioinformatic Analyses of Lipid Metabolism Implicate a Sporophytic Signaling Network Supporting Pollen Development in Arabidopsis Mol Plant, June 25, 2008; (2008) ssn027v1. [Abstract] [Full Text] [PDF]

				D.-S. Zhang, W.-Q. Liang, Z. Yuan, N. Li, J. Shi, J. Wang, Y.-M. Liu, W.-J. Yu, and D.-B. Zhang Tapetum Degeneration Retardation is Critical for Aliphatic Metabolism and Gene Regulation during Rice Pollen Development Mol Plant, June 23, 2008; (2008) ssn028v1. [Abstract] [Full Text] [PDF]

				Y.-F. Guan, X.-Y. Huang, J. Zhu, J.-F. Gao, H.-X. Zhang, and Z.-N. Yang RUPTURED POLLEN GRAIN1, a Member of the MtN3/saliva Gene Family, Is Crucial for Exine Pattern Formation and Cell Integrity of Microspores in Arabidopsis Plant Physiology, June 1, 2008; 147(2): 852 - 863. [Abstract] [Full Text] [PDF]

				C. Yang, G. Vizcay-Barrena, K. Conner, and Z. A. Wilson MALE STERILITY1 Is Required for Tapetal Development and Pollen Wall Biosynthesis PLANT CELL, November 1, 2007; 19(11): 3530 - 3548. [Abstract] [Full Text] [PDF]