First published online December 7, 2007; 10.1105/tpc.107.055160
The Plant Cell 19:3864-3875 (2007)
© 2007 American Society of Plant Biologists
A CRM Domain Protein Functions Dually in Group I and Group II Intron Splicing in Land Plant Chloroplasts[W]
Yukari Asakura and
Alice Barkan1
Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403
1 Address correspondence to abarkan{at}uoregon.edu.
The CRM domain is a recently recognized RNA binding domain found in three group II intron splicing factors in chloroplasts, in a bacterial protein that associates with ribosome precursors, and in a family of uncharacterized proteins in plants. To elucidate the functional repertoire of proteins with CRM domains, we studied CFM2 (for CRM Family Member 2), which harbors four CRM domains. RNA coimmunoprecipitation assays showed that CFM2 in maize (Zea mays) chloroplasts is associated with the group I intron in pre-trnL-UAA and group II introns in the ndhA and ycf3 pre-mRNAs. T-DNA insertions in the Arabidopsis thaliana ortholog condition a defective-seed phenotype (strong allele) or chlorophyll-deficient seedlings with impaired splicing of the trnL group I intron and the ndhA, ycf3-int1, and clpP-int2 group II introns (weak alleles). CFM2 and two previously described CRM proteins are bound simultaneously to the ndhA and ycf3-int1 introns and act in a nonredundant fashion to promote their splicing. With these findings, CRM domain proteins are implicated in the activities of three classes of catalytic RNA: group I introns, group II introns, and 23S rRNA.
Related articles in Plant Cell:
- Chloroplast Intron Splicing Mechanisms
- Nancy A. Eckardt
Plant Cell 2007 19: 3838.
[Full Text]
This article has been cited by other articles:
|
|
|
|
 
H.-S. Jung and J. Chory
Signaling between Chloroplasts and the Nucleus: Can a Systems Biology Approach Bring Clarity to a Complex and Highly Regulated Pathway?
Plant Physiology,
February 1, 2010;
152(2):
453 - 459.
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Keren, A. Bezawork-Geleta, M. Kolton, I. Maayan, E. Belausov, M. Levy, A. Mett, D. Gidoni, F. Shaya, and O. Ostersetzer-Biran
AtnMat2, a nuclear-encoded maturase required for splicing of group-II introns in Arabidopsis mitochondria
RNA,
December 1, 2009;
15(12):
2299 - 2311.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. S. Kroeger, K. P. Watkins, G. Friso, K. J. van Wijk, and A. Barkan
A plant-specific RNA-binding domain revealed through analysis of chloroplast group II intron splicing
PNAS,
March 17, 2009;
106(11):
4537 - 4542.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Asakura, O. A. Bayraktar, and A. Barkan
Two CRM protein subfamilies cooperate in the splicing of group IIB introns in chloroplasts
RNA,
November 1, 2008;
14(11):
2319 - 2332.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Beick, C. Schmitz-Linneweber, R. Williams-Carrier, B. Jensen, and A. Barkan
The Pentatricopeptide Repeat Protein PPR5 Stabilizes a Specific tRNA Precursor in Maize Chloroplasts
Mol. Cell. Biol.,
September 1, 2008;
28(17):
5337 - 5347.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Prikryl, K. P. Watkins, G. Friso, K. J. van Wijk, and A. Barkan
A member of the Whirly family is a multifunctional RNA- and DNA-binding protein that is essential for chloroplast biogenesis
Nucleic Acids Res.,
September 1, 2008;
36(16):
5152 - 5165.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Keren, L. Klipcan, A. Bezawork-Geleta, M. Kolton, F. Shaya, and O. Ostersetzer-Biran
Characterization of the Molecular Basis of Group II Intron RNA Recognition by CRS1-CRM Domains
J. Biol. Chem.,
August 22, 2008;
283(34):
23333 - 23342.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
