Plant Cell email content delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online February 2, 2007; 10.1105/tpc.106.044461

The Plant Cell 19:640-655 (2007)
© 2007 American Society of Plant Biologists

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
19/2/640    most recent
tpc.106.044461v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (11)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vidal, G.
Right arrow Articles by De Paepe, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vidal, G.
Right arrow Articles by De Paepe, R.
Agricola
Right arrow Articles by Vidal, G.
Right arrow Articles by De Paepe, R.

Lack of Respiratory Chain Complex I Impairs Alternative Oxidase Engagement and Modulates Redox Signaling during Elicitor-Induced Cell Death in Tobacco

Guillaume Vidala, Miquel Ribas-Carbob, Marie Garmiera, Guy Dubertreta, Allan G. Rasmussonc, Chantal Mathieua, Christine H. Foyerd and Rosine De Paepea,1

a Laboratoire Mitochondries et Métabolisme, Institut de Biotechnologie des Plantes, Unité Mixte de Recherche, Centre National de la Recherche Scientifique 8618, Université Paris Sud, 91 405 Orsay, France
b Grup de Recerca en Biologia de les Plantes en Condicions Mediterrànies, Departament de Biologia, Universitat de les Illes Balears, 07122 Palma de Mallorca, Spain
c Department of Cell and Organism Biology, Lund University, Sölvegatan 35B, SE-223 62 Lund, Sweden
d Crop Performance and Improvement Division, Rothamsted Research, Harpenden, Herts, AL5 2 JQ, United Kingdom

1 To whom correspondence should be addressed. E-mail rosine.de-paepe{at}u-psud.fr; fax 33-1-69-15-3425.

Alternative oxidase (AOX) functions in stress resistance by preventing accumulation of reactive oxygen species (ROS), but little is known about in vivo partitioning of electron flow between AOX and the cytochrome pathway. We investigated the relationships between AOX expression and in vivo activity in Nicotiana sylvestris and the complex I–deficient CMSII mutant in response to a cell death elicitor. While a specific AOX1 isoform in the active reduced state was constitutively overexpressed in CMSII, partitioning through the alternative pathway was similar to the wild type. Lack of correlation between AOX content and activity indicates severe metabolic constraints in nonstressed mutant leaves. The bacterial elicitor harpin NEa induced similar timing and extent of cell death and a twofold respiratory burst in both genotypes with little change in AOX amounts. However, partitioning to AOX was increased twofold in the wild type but remained unchanged in CMSII. Oxidative phosphorylation modeling indicated a twofold ATP increase in both genotypes. By contrast, mitochondrial superoxide dismutase activity and reduced forms of ascorbate and glutathione were higher in CMSII than in the wild type. These results demonstrate genetically programmed flexibility of plant respiratory routes and antioxidants in response to elicitors and suggest that sustained ATP production, rather than AOX activity by itself or mitochondrial ROS, might be important for in planta cell death.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
F. Van Breusegem, J. Bailey-Serres, and R. Mittler
Unraveling the Tapestry of Networks Involving Reactive Oxygen Species in Plants
Plant Physiology, July 1, 2008; 147(3): 978 - 984.
[Full Text] [PDF]

Home page
 J Exp BotHome page
N. M. Grant, R. E. Miller, J. R. Watling, and S. A. Robinson
Synchronicity of thermogenic activity, alternative pathway respiratory flux, AOX protein content, and carbohydrates in receptacle tissues of sacred lotus during floral development
J. Exp. Bot., February 4, 2008; (2008) erm333v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
Y.-J. Liu, F. E. B. Norberg, A. Szilagyi, R. De Paepe, H.-E. Akerlund, and A. G. Rasmusson
The Mitochondrial External NADPH Dehydrogenase Modulates the Leaf NADPH/NADP+ Ratio in Transgenic Nicotiana sylvestris
Plant Cell Physiol., February 1, 2008; 49(2): 251 - 263.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Garmier, P. Priault, G. Vidal, S. Driscoll, R. Djebbar, M. Boccara, C. Mathieu, C. H. Foyer, and R. De Paepe
Light and Oxygen Are Not Required for Harpin-induced Cell Death
J. Biol. Chem., December 28, 2007; 282(52): 37556 - 37566.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
A. F. de Longevialle, E. H. Meyer, C. Andres, N. L. Taylor, C. Lurin, A. H. Millar, and I. D. Small
The Pentatricopeptide Repeat Gene OTP43 Is Required for trans-Splicing of the Mitochondrial nad1 Intron 1 in Arabidopsis thaliana
PLANT CELL, October 1, 2007; 19(10): 3256 - 3265.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2007 by the American Society of Plant Biologists