First published online March 30, 2007; 10.1105/tpc.107.050385
The Plant Cell 19:750-766 (2007)
© 2007 American Society of Plant Biologists
Elaboration of B Gene Function to Include the Identity of Novel Floral Organs in the Lower Eudicot Aquilegia[W]
Elena M. Kramer1,
Lynn Holappa,
Billie Gould,
M. Alejandra Jaramillo2,
Dimitriy Setnikov3 and
Philip M. Santiago4
Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts 02138
1 To whom correspondence should be addressed. E-mail ekramer{at}oeb.harvard.edu; fax 617-496-5854.
The basal eudicot Aquilegia (columbine) has an unusual floral structure that includes two morphologically distinct whorls of petaloid organs and a clearly differentiated fifth organ type, the staminodium. In this study, we have sought to determine how Aquilegia homologs of the B class genes APETALA3 (AP3) and PISTILLATA (PI) contribute to these novel forms of organ identity. Detailed expression analyses of the three AP3 paralogs and one PI homolog in wild-type and floral homeotic mutant lines reveal complex patterns that suggest that canonical B class function has been elaborated in Aquilegia. Yeast two-hybrid studies demonstrate that the protein products of Aquilegia's AP3 and PI homologs can form heterodimers, much like what has been observed for their core eudicot homologs. Downregulation of AqvPI using virus-induced gene silencing indicates that in addition to petal and stamen identity, this locus is essential to staminodial identity but may not control the identity of the petaloid sepals. Our findings show that preexisting floral organ identity programs can be partitioned and modified to produce additional organ types. In addition, they indicate that some types of petaloid organs are not entirely dependent on AP3/PI homologs for their identity.
This article has been cited by other articles:
|
|
|
|
 
E. M. Kramer and S. A. Hodges
Aquilegia as a model system for the evolution and ecology of petals
Phil Trans R Soc B,
February 12, 2010;
365(1539):
477 - 490.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-Y. Chang, N.-H. Kao, J.-Y. Li, W.-H. Hsu, Y.-L. Liang, J.-W. Wu, and C.-H. Yang
Characterization of the Possible Roles for B Class MADS Box Genes in Regulation of Perianth Formation in Orchid
Plant Physiology,
February 1, 2010;
152(2):
837 - 853.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Viaene, D. Vekemans, V. F. Irish, A. Geeraerts, S. Huysmans, S. Janssens, E. Smets, and K. Geuten
Pistillata--Duplications as a Mode for Floral Diversification in (Basal) Asterids
Mol. Biol. Evol.,
November 1, 2009;
26(11):
2627 - 2645.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Jabbour, L. P. Ronse De Craene, S. Nadot, and C. Damerval
Establishment of zygomorphy on an ontogenic spiral and evolution of perianth in the tribe Delphinieae (Ranunculaceae)
Ann. Bot.,
October 1, 2009;
104(5):
809 - 822.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Shan, L. Zahn, S. Guindon, P. K. Wall, H. Kong, H. Ma, C. W. dePamphilis, and J. Leebens-Mack
Evolution of Plant MADS Box Transcription Factors: Evidence for Shifts in Selection Associated with Early Angiosperm Diversification and Concerted Gene Duplications
Mol. Biol. Evol.,
October 1, 2009;
26(10):
2229 - 2244.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. A. Warner, P. J. Rudall, and M. W. Frohlich
Environmental control of sepalness and petalness in perianth organs of waterlilies: a new Mosaic Theory for the evolutionary origin of a differentiated perianth
J. Exp. Bot.,
August 1, 2009;
60(12):
3559 - 3574.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. C. Hileman and V. F. Irish
More is better: the uses of developmental genetic data to reconstruct perianth evolution
Am. J. Botany,
January 1, 2009;
96(1):
83 - 95.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. A. Rasmussen, E. M. Kramer, and E. A. Zimmer
One size fits all? Molecular evidence for a commonly inherited petal identity program in Ranunculales
Am. J. Botany,
January 1, 2009;
96(1):
96 - 109.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Coupland
Mechanisms and variation in plant development: sorting the wood from the trees in Vermont
Development,
December 1, 2008;
135(23):
3813 - 3816.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Compiled by, F. Tooke, T. Chiurugwi, and N. Battey
Flowering Newsletter bibliography for 2007
J. Exp. Bot.,
July 18, 2008;
(2008)
ern109v1.
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Drea, L. C. Hileman, G. de Martino, and V. F. Irish
Functional analyses of genetic pathways controlling petal specification in poppy
Development,
December 1, 2007;
134(23):
4157 - 4166.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
