First published online April 20, 2007; 10.1105/tpc.106.047076
The Plant Cell 19:1278-1294 (2007)
© 2007 American Society of Plant Biologists
The Transcription Factor WIN1/SHN1 Regulates Cutin Biosynthesis in Arabidopsis thaliana[W]
Rubini Kannangaraa,1,
Caroline Branigana,1,
Yan Liua,2,
Teresa Penfielda,
Vijaya Raob,
Grégory Mouillec,
Herman Höftec,
Markus Paulyd,3,
José Luis Riechmannb and
Pierre Brouna,4
a Centre for Novel Agricultural Products, Department of Biology, University of York, York YO10 5YW, United Kingdom
b Millard and Muriel Jacobs Genetics and Genomics Laboratory, California Institute of Technology, Division of Biology 156-29, Pasadena, California 91125
c Laboratoire de Biologie Cellulaire, Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, 78026 Versailles Cedex, France
d Max-Planck-Institute for Molecular Plant Physiology, 14476 Golm, Germany
4 To whom correspondence should be addressed. E-mail pb22{at}york.ac.uk; fax 44-1904-328762.
The composition and permeability of the cuticle has a large influence on its ability to protect the plant against various forms of biotic and abiotic stress. WAX INDUCER1 (WIN1) and related transcription factors have recently been shown to trigger wax production, enhance drought tolerance, and modulate cuticular permeability when overexpressed in Arabidopsis thaliana. We found that WIN1 influences the composition of cutin, a polyester that forms the backbone of the cuticle. WIN1 overexpression induces compositional changes and an overall increase in cutin production in vegetative and reproductive organs, while its downregulation has the opposite effect. Changes in cutin composition are preceded by the rapid and coordinated induction of several genes known or likely to be involved in cutin biosynthesis. This transcriptional response is followed after a delay by the induction of genes associated with wax biosynthesis, suggesting that the regulation of cutin and wax production by WIN1 is a two-step process. We demonstrate that at least one of the cutin pathway genes, which encodes long-chain acyl-CoA synthetase LACS2, is likely to be directly targeted by WIN1. Overall, our results suggest that WIN1 modulates cuticle permeability in Arabidopsis by regulating genes encoding cutin pathway enzymes.
This article has been cited by other articles:

|
 |

|
 |
 
D. Panikashvili, J. X. Shi, S. Bocobza, R. B. Franke, L. Schreiber, and A. Aharoni
The Arabidopsis DSO/ABCG11 Transporter Affects Cutin Metabolism in Reproductive Organs and Suberin in Roots
Mol Plant,
December 24, 2009;
(2009)
ssp103v1.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
Y. Li-Beisson, M. Pollard, V. Sauveplane, F. Pinot, J. Ohlrogge, and F. Beisson
Nanoridges that characterize the surface morphology of flowers require the synthesis of cutin polyester
PNAS,
December 22, 2009;
106(51):
22008 - 22013.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
A. Kamigaki, M. Kondo, S. Mano, M. Hayashi, and M. Nishimura
Suppression of Peroxisome Biogenesis Factor 10 Reduces Cuticular Wax Accumulation by Disrupting the ER Network in Arabidopsis thaliana
Plant Cell Physiol.,
December 1, 2009;
50(12):
2034 - 2046.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
D. Panikashvili, J. X. Shi, L. Schreiber, and A. Aharoni
The Arabidopsis DCR Encoding a Soluble BAHD Acyltransferase Is Required for Cutin Polyester Formation and Seed Hydration Properties
Plant Physiology,
December 1, 2009;
151(4):
1773 - 1789.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. T. Waters, P. Wang, M. Korkaric, R. G. Capper, N. J. Saunders, and J. A. Langdale
GLK Transcription Factors Coordinate Expression of the Photosynthetic Apparatus in Arabidopsis
PLANT CELL,
April 1, 2009;
21(4):
1109 - 1128.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. Mintz-Oron, T. Mandel, I. Rogachev, L. Feldberg, O. Lotan, M. Yativ, Z. Wang, R. Jetter, I. Venger, A. Adato, et al.
Gene Expression and Metabolism in Tomato Fruit Surface Tissues
Plant Physiology,
June 1, 2008;
147(2):
823 - 851.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
K. Century, T. L. Reuber, and O. J. Ratcliffe
Regulating the Regulators: The Future Prospects for Transcription-Factor-Based Agricultural Biotechnology Products
Plant Physiology,
May 1, 2008;
147(1):
20 - 29.
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. Taketa, S. Amano, Y. Tsujino, T. Sato, D. Saisho, K. Kakeda, M. Nomura, T. Suzuki, T. Matsumoto, K. Sato, et al.
Barley grain with adhering hulls is controlled by an ERF family transcription factor gene regulating a lipid biosynthesis pathway
PNAS,
March 11, 2008;
105(10):
4062 - 4067.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. Raffaele, F. Vailleau, A. Leger, J. Joubes, O. Miersch, C. Huard, E. Blee, S. Mongrand, F. Domergue, and D. Roby
A MYB Transcription Factor Regulates Very-Long-Chain Fatty Acid Biosynthesis for Activation of the Hypersensitive Cell Death Response in Arabidopsis
PLANT CELL,
March 1, 2008;
20(3):
752 - 767.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
Y. Li, F. Beisson, A. J. K. Koo, I. Molina, M. Pollard, and J. Ohlrogge
Identification of acyltransferases required for cutin biosynthesis and production of cutin with suberin-like monomers
PNAS,
November 13, 2007;
104(46):
18339 - 18344.
[Abstract]
[Full Text]
[PDF]
|
 |
|
|
|