OPEN ACCESS ARTICLE

This Article Free via Open Access: OA OA Full Text Full Text (PDF) Supplemental Data OA All Versions of this Article: 19/4/1415    most recent tpc.106.042291v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (18) Citing Articles via Google Scholar Google Scholar Articles by Quan, R. Articles by Guo, Y. Search for Related Content PubMed PubMed Citation Articles by Quan, R. Articles by Guo, Y. Agricola Articles by Quan, R. Articles by Guo, Y.

SCABP8/CBL10, a Putative Calcium Sensor, Interacts with the Protein Kinase SOS2 to Protect Arabidopsis Shoots from Salt Stress^[W],[OA]

^a National Institute of Biological Sciences, Beijing 102206, China
^b Instituto de Recursos Naturales y Agrobiología, Consejo Superior de Investigaciones Científicas, Sevilla 41012, Spain
^c Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China

¹ To whom correspondence should be addressed. E-mail guoyan{at}nibs.ac.cn; fax 86-10-80726671.

The SOS (for Salt Overly Sensitive) pathway plays essential roles in conferring salt tolerance in Arabidopsis thaliana. Under salt stress, the calcium sensor SOS3 activates the kinase SOS2 that positively regulates SOS1, a plasma membrane sodium/proton antiporter. We show that SOS3 acts primarily in roots under salt stress. By contrast, the SOS3 homolog SOS3-LIKE CALCIUM BINDING PROTEIN8 (SCABP8)/CALCINEURIN B-LIKE10 functions mainly in the shoot response to salt toxicity. While root growth is reduced in sos3 mutants in the presence of NaCl, the salt sensitivity of scabp8 is more prominent in shoot tissues. SCABP8 is further shown to bind calcium, interact with SOS2 both in vitro and in vivo, recruit SOS2 to the plasma membrane, enhance SOS2 activity in a calcium-dependent manner, and activate SOS1 in yeast. In addition, sos3 scabp8 and sos2 scabp8 display a phenotype similar to sos2, which is more sensitive to salt than either sos3 or scabp8 alone. Overexpression of SCABP8 in sos3 partially rescues the sos3 salt-sensitive phenotype. However, overexpression of SOS3 fails to complement scabp8. These results suggest that SCABP8 and SOS3 are only partially redundant in their function, and each plays additional and unique roles in the plant salt stress response.

This article has been cited by other articles:

				H. Lin, Y. Yang, R. Quan, I. Mendoza, Y. Wu, W. Du, S. Zhao, K. S. Schumaker, J. M. Pardo, and Y. Guo Phosphorylation of SOS3-LIKE CALCIUM BINDING PROTEIN8 by SOS2 Protein Kinase Stabilizes Their Protein Complex and Regulates Salt Tolerance in Arabidopsis PLANT CELL, May 1, 2009; 21(5): 1607 - 1619. [Abstract] [Full Text] [PDF]

				H. Fujii and J.-K. Zhu An Autophosphorylation Site of the Protein Kinase SOS2 Is Important for Salt Tolerance in Arabidopsis Mol Plant, January 6, 2009; (2009) ssn087v1. [Abstract] [Full Text] [PDF]

				Q. Yang, Z.-Z. Chen, X.-F. Zhou, H.-B. Yin, X. Li, X.-F. Xin, X.-H. Hong, J.-K. Zhu, and Z. Gong Overexpression of SOS (Salt Overly Sensitive) Genes Increases Salt Tolerance in Transgenic Arabidopsis Mol Plant, January 1, 2009; 2(1): 22 - 31. [Abstract] [Full Text] [PDF]

				S.-I. Oh, J. Park, S. Yoon, Y. Kim, S. Park, M. Ryu, M. J. Nam, S. H. Ok, J.-K. Kim, J.-S. Shin, et al. The Arabidopsis Calcium Sensor Calcineurin B-Like 3 Inhibits the 5'-Methylthioadenosine Nucleosidase in a Calcium-Dependent Manner Plant Physiology, December 1, 2008; 148(4): 1883 - 1896. [Abstract] [Full Text] [PDF]

				X. Chen, W.-H. Lin, Y. Wang, S. Luan, and H.-W. Xue An Inositol Polyphosphate 5-Phosphatase Functions in PHOTOTROPIN1 Signaling in Arabidopis by Altering Cytosolic Ca2+ PLANT CELL, February 1, 2008; 20(2): 353 - 366. [Abstract] [Full Text] [PDF]

				H. Chen, Y. Zou, Y. Shang, H. Lin, Y. Wang, R. Cai, X. Tang, and J.-M. Zhou Firefly Luciferase Complementation Imaging Assay for Protein-Protein Interactions in Plants Plant Physiology, February 1, 2008; 146(2): 368 - 376. [Abstract] [Full Text] [PDF]

				G. Batelli, P. E. Verslues, F. Agius, Q. Qiu, H. Fujii, S. Pan, K. S. Schumaker, S. Grillo, and J.-K. Zhu SOS2 Promotes Salt Tolerance in Part by Interacting with the Vacuolar H+-ATPase and Upregulating Its Transport Activity Mol. Cell. Biol., November 15, 2007; 27(22): 7781 - 7790. [Abstract] [Full Text] [PDF]