First published online May 18, 2007; 10.1105/tpc.106.049239
The Plant Cell 19:1537-1548 (2007)
© 2007 American Society of Plant Biologists
NUCLEAR PORE ANCHOR, the Arabidopsis Homolog of Tpr/Mlp1/Mlp2/Megator, Is Involved in mRNA Export and SUMO Homeostasis and Affects Diverse Aspects of Plant Development[W]
Xianfeng Morgan Xu,
Annkatrin Rose1,
Sivaramakrishnan Muthuswamy,
Sun Yong Jeong2,
Sowmya Venkatakrishnan,
Qiao Zhao and
Iris Meier3
Plant Biotechnology Center and Department of Plant Cellular and Molecular Biology, Ohio State University, Columbus, Ohio 43210
3 To whom correspondence should be addressed. E-mail meier.56{at}osu.edu; fax 614-292-5379.
Vertebrate Tpr and its yeast homologs Mlp1/Mlp2, long coiled-coil proteins of nuclear pore inner basket filaments, are involved in mRNA export, telomere organization, spindle pole assembly, and unspliced RNA retention. We identified Arabidopsis thaliana NUCLEAR PORE ANCHOR (NUA) encoding a 237-kD protein with similarity to Tpr. NUA is located at the inner surface of the nuclear envelope in interphase and in the vicinity of the spindle in prometaphase. Four T-DNA insertion lines were characterized, which comprise an allelic series of increasing severity for several correlating phenotypes, such as early flowering under short days and long days, increased abundance of SUMO conjugates, altered expression of several flowering regulators, and nuclear accumulation of poly(A)+ RNA. nua mutants phenocopy mutants of EARLY IN SHORT DAYS4 (ESD4), an Arabidopsis SUMO protease concentrated at the nuclear periphery. nua esd4 double mutants resemble nua and esd4 single mutants, suggesting that the two proteins act in the same pathway or complex, supported by yeast two-hybrid interaction. Our data indicate that NUA is a component of nuclear pore-associated steps of sumoylation and mRNA export in plants and that defects in these processes affect the signaling events of flowering time regulation and additional developmental processes.
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Compiled by, F. Tooke, T. Chiurugwi, and N. Battey
Flowering Newsletter bibliography for 2007
J. Exp. Bot.,
July 18, 2008;
(2008)
ern109v1.
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