Plant Cell Hybrigenics The Protein Interactions Experts
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


First published online June 8, 2007; 10.1105/tpc.106.048777

The Plant Cell 19:1826-1837 (2007)
© 2007 American Society of Plant Biologists

OPEN ACCESS ARTICLE
This Article
Free via Open Access: OA
Right arrow OA Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrowOA All Versions of this Article:
19/6/1826    most recent
tpc.106.048777v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (16)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wu, G.
Right arrow Articles by Spalding, E. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wu, G.
Right arrow Articles by Spalding, E. P.
Agricola
Right arrow Articles by Wu, G.
Right arrow Articles by Spalding, E. P.

Mutations in Arabidopsis Multidrug Resistance-Like ABC Transporters Separate the Roles of Acropetal and Basipetal Auxin Transport in Lateral Root Development[W],[OA]

Guosheng Wu, Daniel R. Lewis and Edgar P. Spalding1

Department of Botany, University of Wisconsin, Madison, Wisconsin 53706

1 To whom correspondence should be addressed. E-mail spalding{at}wisc.edu; fax 608-262-7509.

Auxin affects the shape of root systems by influencing elongation and branching. Because multidrug resistance (MDR)-like ABC transporters participate in auxin transport, they may be expected to contribute to root system development. This reverse genetic study of Arabidopsis thaliana roots shows that MDR4-mediated basipetal auxin transport did not affect root elongation or branching. However, impaired acropetal auxin transport due to mutation of the MDR1 gene caused 21% of nascent lateral roots to arrest their growth and the remainder to elongate 50% more slowly than the wild type. Reporter gene analyses indicated a severe auxin deficit in the apex of mdr1 but not mdr4 lateral roots. The mdr1 deficit was explained by 40% less acropetal auxin transport within the mdr1 lateral roots. The slow elongation of mdr1 lateral roots was rescued by auxin and phenocopied in the wild type by an inhibitor of polar auxin transport. Confocal microscopy analysis of a functional green fluorescent protein–MDR1 translational fusion showed the protein to be auxin inducible and present in the tissues responsible for acropetal transport in the primary root. The protein also accumulated in lateral root primordia and later in the tissues responsible for acropetal transport within the lateral root, fully supporting the conclusion that auxin levels established by MDR1-dependent acropetal transport control lateral root growth rate to influence root system architecture.




This article has been cited by other articles:


Home page
J Exp BotHome page
B. Titapiwatanakun and A. S. Murphy
Post-transcriptional regulation of auxin transport proteins: cellular trafficking, protein phosphorylation, protein maturation, ubiquitination, and membrane composition
J. Exp. Bot., March 1, 2009; 60(4): 1093 - 1107.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
D. Santelia, S. Henrichs, V. Vincenzetti, M. Sauer, L. Bigler, M. Klein, A. Bailly, Y. Lee, J. Friml, M. Geisler, et al.
Flavonoids Redirect PIN-mediated Polar Auxin Fluxes during Root Gravitropic Responses
J. Biol. Chem., November 7, 2008; 283(45): 31218 - 31226.
[Abstract] [Full Text] [PDF]


Home page
DevelopmentHome page
J. Mravec, M. Kubes, A. Bielach, V. Gaykova, J. Petrasek, P. Skupa, S. Chand, E. Benkova, E. Zazimalova, and J. Friml
Interaction of PIN and PGP transport mechanisms in auxin distribution-dependent development
Development, October 15, 2008; 135(20): 3345 - 3354.
[Abstract] [Full Text] [PDF]


Home page
J Exp BotHome page
K. Guo, K. Xia, and Z.-M. Yang
Regulation of tomato lateral root development by carbon monoxide and involvement in auxin and nitric oxide
J. Exp. Bot., September 1, 2008; 59(12): 3443 - 3452.
[Abstract] [Full Text] [PDF]


Home page
Plant CellHome page
D. R. Lewis, N. D. Miller, B. L. Splitt, G. Wu, and E. P. Spalding
Separating the Roles of Acropetal and Basipetal Auxin Transport on Gravitropism with Mutations in Two Arabidopsis Multidrug Resistance-Like ABC Transporter Genes
PLANT CELL, June 1, 2007; 19(6): 1838 - 1850.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2007 by the American Society of Plant Biologists