This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 19/8/2569    most recent tpc.107.053538v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (6) Citing Articles via Google Scholar Google Scholar Articles by Holding, D. R. Articles by Larkins, B. A. Search for Related Content PubMed PubMed Citation Articles by Holding, D. R. Articles by Larkins, B. A. Agricola Articles by Holding, D. R. Articles by Larkins, B. A.

The Maize Floury1 Gene Encodes a Novel Endoplasmic Reticulum Protein Involved in Zein Protein Body Formation^[W]

^a Department of Plant Sciences, University of Arizona, Tucson, Arizona, 85721
^b Department of Botany, University of Wisconsin, Madison, Wisconsin, 53706
^c DuPont Crop Genetics Research, Experimental Station, Wilmington, Delaware 19880-0353
^d Pioneer Hi-Bred International, Johnston, Iowa, 50131-1004

¹ Address correspondence to larkins{at}ag.arizona.edu.

The maize (Zea mays) floury1 (fl1) mutant was first reported almost 100 years ago, but its molecular identity has remained unknown. We report the cloning of Fl1, which encodes a novel zein protein body membrane protein with three predicted transmembrane domains and a C-terminal plant-specific domain of unknown function (DUF593). In wild-type endosperm, the FL1 protein accumulates at a high level during the period of zein synthesis and protein body development and declines to a low level at kernel maturity. Immunogold labeling showed that FL1 resides in the endoplasmic reticulum surrounding the protein body. Zein protein bodies in fl1 mutants are of normal size, shape, and abundance. However, mutant protein bodies ectopically accumulate 22-kD -zeins in the -zein–rich periphery and center of the core, rather than their normal discrete location in a ring at outer edge of the core. The 19-kD -zein is uniformly distributed throughout the core in wild-type protein bodies, and this distribution is unaffected in fl1 mutants. Pairwise yeast two-hybrid experiments showed that FL1 DUF593 interacts with the 22-kD -zein. Results of these studies suggest that FL1 participates in protein body formation by facilitating the localization of 22-kD -zein and that this is essential for the formation of vitreous endosperm.

This article has been cited by other articles:

				P. A. Sabelli and B. A. Larkins The Development of Endosperm in Grasses Plant Physiology, January 1, 2009; 149(1): 14 - 26. [Full Text] [PDF]

				Y. Ma, T. L. Slewinski, R. F. Baker, and D. M. Braun Tie-dyed1 Encodes a Novel, Phloem-Expressed Transmembrane Protein That Functions in Carbohydrate Partitioning Plant Physiology, January 1, 2009; 149(1): 181 - 194. [Abstract] [Full Text] [PDF]

				K. Yamada, A. J. Nagano, M. Nishina, I. Hara-Nishimura, and M. Nishimura NAI2 Is an Endoplasmic Reticulum Body Component That Enables ER Body Formation in Arabidopsis thaliana PLANT CELL, September 1, 2008; 20(9): 2529 - 2540. [Abstract] [Full Text] [PDF]