THE PLANT CELL, Vol 2, Issue 1 39-49, Copyright © 1990 by American Society of Plant Biologists
Brassica S-Proteins Accumulate in the Intercellular Matrix along the Path of Pollen Tubes in Transgenic Tobacco Pistils
M. K. Kandasamy, K. G. Dwyer, D. J. Paolillo, R. C. Doney, J. B. Nasrallah and M. E. Nasrallah
Section of Plant Biology, Division of Biological Sciences, Cornell University, Ithaca, New York 14853
A tobacco plant transformed with a Brassica oleracea SLG-22 gene was
analyzed by immunocytochemical methods to determine the localization of the
transgene-encoded protein product. Immunolabeling was observed in the
pistil along the path followed by pollen tubes after pollination. S-antigen
accumulated in the intercellular matrix of the transmitting tissue of the
style and its continuation in the basal portion of the stigma and outside a
few special cells of the placental epidermis of the ovary. This pattern of
S-antigen distribution closely resembles that described for the
S-associated glycoproteins of self-incompatible Nicotiana alata and differs
from its distribution in B. oleracea.
