Plant Cell Blood
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (150)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Denecke, J.
Right arrow Articles by Deblaere, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Denecke, J.
Right arrow Articles by Deblaere, R.
Agricola
Right arrow Articles by Denecke, J.
Right arrow Articles by Deblaere, R.

THE PLANT CELL, Vol 2, Issue 1 51-59, Copyright © 1990 by American Society of Plant Biologists

RESEARCH ARTICLES

Protein Secretion in Plant Cells Can Occur via a Default Pathway

J. Denecke, J. Botterman and R. Deblaere
Plant Genetic Systems N.V., J. Plateaustraat 22, 9000 Gent, Belgium

To study protein secretion in plant cells, we established and evaluated a model system based on transient synthesis of heterologous proteins in tobacco protoplasts. We show that the nonsecretory enzymes phosphinothricin acetyl transferase, neomycin phosphotransferase II, and [beta]-glucuronidase are secreted when targeted to the lumen of the endoplasmic reticulum by signal peptide-mediated translocation. These data are consistent with the view that secretion can occur independent of active sorting mechanisms by nonspecific migration through the exocytic pathway. However, the rate of secretion differs significantly among these enzymes. Furthermore, the presence of signal sequences was found to be correlated with a reduction of the levels of the encoded gene products. This is the result of post-transcriptional events that limit either synthesis or stability of the proteins in vivo.


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
E. Rojo and J. Denecke
What Is Moving in the Secretory Pathway of Plants?
Plant Physiology, August 1, 2008; 147(4): 1493 - 1503.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. L. Hanton, L. Chatre, L. Renna, L. A. Matheson, and F. Brandizzi
De Novo Formation of Plant Endoplasmic Reticulum Export Sites Is Membrane Cargo Induced and Signal Mediated
Plant Physiology, April 1, 2007; 143(4): 1640 - 1650.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
F. Wen, H. D. VanEtten, G. Tsaprailis, and M. C. Hawes
Extracellular Proteins in Pea Root Tip and Border Cell Exudates
Plant Physiology, February 1, 2007; 143(2): 773 - 783.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
P. Moreau, F. Brandizzi, S. Hanton, L. Chatre, S. Melser, C. Hawes, and B. Satiat-Jeunemaitre
The plant ER-Golgi interface: a highly structured and dynamic membrane complex
J. Exp. Bot., January 1, 2007; 58(1): 49 - 64.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Komarnytsky, N. Borisjuk, N. Yakoby, A. Garvey, and I. Raskin
Cosecretion of Protease Inhibitor Stabilizes Antibodies Produced by Plant Roots
Plant Physiology, August 1, 2006; 141(4): 1185 - 1193.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
H. Yasuda, Y. Hayashi, T. Jomori, and F. Takaiwa
The Correlation between Expression and Localization of a Foreign Gene Product in Rice Endosperm
Plant Cell Physiol., June 1, 2006; 47(6): 756 - 763.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
L. L.P. daSilva, O. Foresti, and J. Denecke
Targeting of the Plant Vacuolar Sorting Receptor BP80 Is Dependent on Multiple Sorting Signals in the Cytosolic Tail
PLANT CELL, June 1, 2006; 18(6): 1477 - 1497.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. Drakakaki, S. Marcel, E. Arcalis, F. Altmann, P. Gonzalez-Melendi, R. Fischer, P. Christou, and E. Stoger
The Intracellular Fate of a Recombinant Protein Is Tissue Dependent
Plant Physiology, June 1, 2006; 141(2): 578 - 586.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
P. Pimpl, J. P. Taylor, C. Snowden, S. Hillmer, D. G. Robinson, and J. Denecke
Golgi-Mediated Vacuolar Sorting of the Endoplasmic Reticulum Chaperone BiP May Play an Active Role in Quality Control within the Secretory Pathway
PLANT CELL, January 1, 2006; 18(1): 198 - 211.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. L. Hanton, L. Renna, L. E. Bortolotti, L. Chatre, G. Stefano, and F. Brandizzi
Diacidic Motifs Influence the Export of Transmembrane Proteins from the Endoplasmic Reticulum in Plant Cells
PLANT CELL, November 1, 2005; 17(11): 3081 - 3093.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
S.-J.-D. Claude, G. Marie-Agnes, R. Catalina, P. Nadine, K.-M. Marie-Christine, N. Jean-Marc, F. Loic, and G. Veronique
Targeting of proConA to the Plant Vacuole depends on its Nine Amino-acid C-terminal Propeptide
Plant Cell Physiol., October 1, 2005; 46(10): 1603 - 1612.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Y.-d. Yang, R. Elamawi, J. Bubeck, R. Pepperkok, C. Ritzenthaler, and D. G. Robinson
Dynamics of COPII Vesicles and the Golgi Apparatus in Cultured Nicotiana tabacum BY-2 Cells Provides Evidence for Transient Association of Golgi Stacks with Endoplasmic Reticulum Exit Sites
PLANT CELL, May 1, 2005; 17(5): 1513 - 1531.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
L. L.P. daSilva, J. P. Taylor, J. L. Hadlington, S. L. Hanton, C. J. Snowden, S. J. Fox, O. Foresti, F. Brandizzi, and J. Denecke
Receptor Salvage from the Prevacuolar Compartment Is Essential for Efficient Vacuolar Protein Targeting
PLANT CELL, January 1, 2005; 17(1): 132 - 148.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. H.H. Borner, D. J. Sherrier, T. Weimar, L. V. Michaelson, N. D. Hawkins, A. MacAskill, J. A. Napier, M. H. Beale, K. S. Lilley, and P. Dupree
Analysis of Detergent-Resistant Membranes in Arabidopsis. Evidence for Plasma Membrane Lipid Rafts
Plant Physiology, January 1, 2005; 137(1): 104 - 116.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. Mainieri, M. Rossi, M. Archinti, M. Bellucci, F. De Marchis, S. Vavassori, A. Pompa, S. Arcioni, and A. Vitale
Zeolin. A New Recombinant Storage Protein Constructed Using Maize {gamma}-Zein and Bean Phaseolin
Plant Physiology, November 1, 2004; 136(3): 3447 - 3456.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
L. L.P. daSilva, E. L. Snapp, J. Denecke, J. Lippincott-Schwartz, C. Hawes, and F. Brandizzi
Endoplasmic Reticulum Export Sites and Golgi Bodies Behave as Single Mobile Secretory Units in Plant Cells
PLANT CELL, July 1, 2004; 16(7): 1753 - 1771.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Holtgrefe, K. P. Bader, P. Horton, R. Scheibe, A. von Schaewen, and J. E. Backhausen
Decreased Content of Leaf Ferredoxin Changes Electron Distribution and Limits Photosynthesis in Transgenic Potato Plants
Plant Physiology, December 1, 2003; 133(4): 1768 - 1778.
[Abstract] [Full Text]

Home page
 ANN BOT (LOND)Home page
U. NEUMANN, F. BRANDIZZI, and C. HAWES
Protein Transport in Plant Cells: In and Out of the Golgi{dagger}
Ann. Bot., August 1, 2003; 92(2): 167 - 180.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
J. L. Hadlington, A. Santoro, J. Nuttall, J. Denecke, J. K-C. Ma, A. Vitale, and L. Frigerio
The C-terminal Extension of a Hybrid Immunoglobulin A/G Heavy Chain Is Responsible for Its Golgi-mediated Sorting to the Vacuole
Mol. Biol. Cell, June 1, 2003; 14(6): 2592 - 2602.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
E. Rojo, V. K. Sharma, V. Kovaleva, N. V. Raikhel, and J. C. Fletcher
CLV3 Is Localized to the Extracellular Space, Where It Activates the Arabidopsis CLAVATA Stem Cell Signaling Pathway
PLANT CELL, May 1, 2002; 14(5): 969 - 977.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
B. A. Phillipson, P. Pimpl, L. L. P. daSilva, A. J. Crofts, J. P. Taylor, A. Movafeghi, D. G. Robinson, and J. Denecke
Secretory Bulk Flow of Soluble Proteins Is Efficient and COPII Dependent
PLANT CELL, September 1, 2001; 13(9): 2005 - 2020.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
K. Tormakangas, J. L. Hadlington, P. Pimpl, S. Hillmer, F. Brandizzi, T. H. Teeri, and J. Denecke
A Vacuolar Sorting Domain May Also Influence the Way in Which Proteins Leave the Endoplasmic Reticulum
PLANT CELL, September 1, 2001; 13(9): 2021 - 2032.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. C. Baldwin, A. S. Karthikeyan, and K. G. Raghothama
LEPS2, a Phosphorus Starvation-Induced Novel Acid Phosphatase from Tomato
Plant Physiology, February 1, 2001; 125(2): 728 - 737.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
A. Vitale and G. Galili
The Endomembrane System and the Problem of Protein Sorting
Plant Physiology, January 1, 2001; 125(1): 115 - 118.
[Full Text]

Home page
 Plant CellHome page
H. Batoko, H.-Q. Zheng, C. Hawes, and I. Moore
A Rab1 GTPase Is Required for Transport between the Endoplasmic Reticulum and Golgi Apparatus and for Normal Golgi Movement in Plants
PLANT CELL, November 1, 2000; 12(11): 2201 - 2218.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
S. Komarnytsky, N. V. Borisjuk, L. G. Borisjuk, M. Z. Alam, and I. Raskin
Production of Recombinant Proteins in Tobacco Guttation Fluid
Plant Physiology, November 1, 2000; 124(3): 927 - 934.
[Abstract] [Full Text]

Home page
 Plant CellHome page
P. Pimpl and J. Denecke
ER Retention of Soluble Proteins: Retrieval, Retention, or Both?
PLANT CELL, September 1, 2000; 12(9): 1517 - 1519.
[Full Text]

Home page
 Plant CellHome page
V. Gomord and L. Faye
Reply: Glycobiology and the Plant Cell--A World of Information
PLANT CELL, September 1, 2000; 12(9): 1519 - 1521.
[Full Text]

Home page
 Appl. Environ. Microbiol.Home page
Q. Yuan, W. Hu, J. J. Pestka, S. Y. He, and L. P. Hart
Expression of a Functional Antizearalenone Single-Chain Fv Antibody in Transgenic Arabidopsis Plants
Appl. Envir. Microbiol., August 1, 2000; 66(8): 3499 - 3505.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
L. Frigerio, N. D. Vine, E. Pedrazzini, M. B. Hein, F. Wang, J. K.-C. Ma, and A. Vitale
Assembly, Secretion, and Vacuolar Delivery of a Hybrid Immunoglobulin in Plants
Plant Physiology, August 1, 2000; 123(4): 1483 - 1494.
[Abstract] [Full Text]

Home page
 Plant CellHome page
A. J. Crofts, N. Leborgne-Castel, S. Hillmer, D. G. Robinson, B. Phillipson, L. E. Carlsson, D. A. Ashford, and J. Denecke
Saturation of the Endoplasmic Reticulum Retention Machinery Reveals Anterograde Bulk Flow
PLANT CELL, November 1, 1999; 11(11): 2233 - 2248.
[Abstract] [Full Text]

Home page
 Plant CellHome page
A. Vitale and J. Denecke
The Endoplasmic Reticulum—Gateway of the Secretory Pathway
PLANT CELL, April 1, 1999; 11(4): 615 - 628.
[Full Text]

Home page
 Plant CellHome page
N. Leborgne-Castel, E. P. W. M. Jelitto-Van Dooren, A. J. Crofts, and J. Denecke
Overexpression of BiP in Tobacco Alleviates Endoplasmic Reticulum Stress
PLANT CELL, March 1, 1999; 11(3): 459 - 470.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
L. Frigerio, M. de Virgilio, A. Prada, F. Faoro, and A. Vitale
Sorting of Phaseolin to the Vacuole Is Saturable and Requires a Short C-Terminal Peptide
PLANT CELL, June 1, 1998; 10(6): 1031 - 1042.
[Abstract] [Full Text]

Home page
 J. Cell Sci.Home page
B. Satiat-Jeunemaitre and C. Hawes
Redistribution of a Golgi glycoprotein in plant cells treated with Brefeldin A
J. Cell Sci., December 1, 1992; 103(4): 1153 - 1166.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 1990 by the American Society of Plant Biologists