|
THE PLANT CELL, Vol 2, Issue 1 71-83, Copyright © 1990 by American Society of Plant Biologists
Transcriptional and Post-Transcriptional Regulation of RNA Levels in Maize Mitochondria
P. M. Finnegan and G. G. Brown
Department of Biology, McGill University, Montreal, Quebec, Canada H3A 1B1
Relatively little is known about the mechanisms that govern the expression
of plant mitochondrial genomes. We have addressed this problem by analyzing
the transcriptional activity of different regions of the maize
mitochondrial genome using both in vivo and isolated mitochondrial
pulse-labeling systems. The regions examined included the protein genes
atpA, atp6, and coxII, the 26S, 18S, and 5S rRNA genes, and sequences
surrounding the rRNA genes. The rRNAs were found to be transcribed at rates
fivefold to 10-fold higher than the protein genes. These rate differences
are comparable with the differences in abundance of these species in the
total or steady-state RNA population. Pulse-labeled RNA unexpectedly
detected transcription of all regions examined, including approximately 21
kilobases of presumed noncoding sequences flanking the rRNA genes for which
stable transcripts were not detected. The results obtained with RNA labeled
for short pulses in vivo and in isolated mitochondria were similar,
suggesting that isolated mitochondria provide a faithful run-on
transcription assay. Our results indicate that the absence in total RNA of
transcripts homologous to a given region of maize mitochondrial DNA does
not necessarily exclude transcriptional activity of that region and that
both transcriptional and post-transcriptional processes play important
roles in maize mitochondrial genome expression.
This article has been cited by other articles:
|
|
|
|
 
J. Forner, B. Weber, S. Thuss, S. Wildum, and S. Binder
Mapping of mitochondrial mRNA termini in Arabidopsis thaliana: t-elements contribute to 5' and 3' end formation
Nucleic Acids Res.,
June 28, 2007;
35(11):
3676 - 3692.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Holec, H. Lange, K. Kuhn, M. Alioua, T. Borner, and D. Gagliardi
Relaxed transcription in Arabidopsis mitochondria is counterbalanced by RNA stability control mediated by polyadenylation and polynucleotide phosphorylase.
Mol. Cell. Biol.,
April 1, 2006;
26(7):
2869 - 2876.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Perrin, H. Lange, J.-M. Grienenberger, and D. Gagliardi
AtmtPNPase is required for multiple aspects of the 18S rRNA metabolism in Arabidopsis thaliana mitochondria
Nucleic Acids Res.,
September 30, 2004;
32(17):
5174 - 5182.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Perrin, E. H. Meyer, M. Zaepfel, Y.-J. Kim, R. Mache, J.-M. Grienenberger, J. M. Gualberto, and D. Gagliardi
Two Exoribonucleases Act Sequentially to Process Mature 3'-Ends of atp9 mRNAs in Arabidopsis Mitochondria
J. Biol. Chem.,
June 11, 2004;
279(24):
25440 - 25446.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. W. Lilly, J. E. Maul, and D. B. Stern
The Chlamydomonas reinhardtii Organellar Genomes Respond Transcriptionally and Post-Transcriptionally to Abiotic Stimuli
PLANT CELL,
November 1, 2002;
14(11):
2681 - 2706.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. S. Lupold, A. G. F. S. Caoile, and D. B. Stern
Genomic context influences the activity of maize mitochondrial cox2 promoters
PNAS,
September 28, 1999;
96(20):
11670 - 11675.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. S. Lupold, A. G. F. S. Caoile, and D. B. Stern
Polyadenylation Occurs at Multiple Sites in Maize Mitochondrial cox2 mRNA and Is Independent of Editing Status
PLANT CELL,
August 1, 1999;
11(8):
1565 - 1578.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
D. S. Lupold, A. G. F. S. Caoile, and D. B. Stern
The Maize Mitochondrial cox2 Gene Has Five Promoters in Two Genomic Regions, Including a Complex Promoter Consisting of Seven Overlapping Units
J. Biol. Chem.,
February 5, 1999;
274(6):
3897 - 3903.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
