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THE PLANT CELL, Vol 2, Issue 10 999-1007, Copyright © 1990 by American Society of Plant Biologists
Activation of a Bean Chitinase Promoter in Transgenic Tobacco Plants by Phytopathogenic Fungi
D. Roby, K. Broglie, R. Cressman, P. Biddle, I. L. Chet and R. Broglie
E.I. Du Pont de Nemours & Company, Agricultural Products Department, Experimental Station, P.O. Box 80402, Wilmington, Delaware 19880
The temporal and spatial expression of a bean chitinase promoter has been
investigated in response to fungal attack. Analysis of transgenic tobacco
plants containing a chimeric gene composed of a 1.7-kilobase fragment
carrying the chitinase 5B gene promoter fused to the coding region of the
gus A gene indicated that the chitinase promoter is activated during attack
by the fungal pathogens Botrytis cinerea, Rhizoctonia solani, and
Sclerotium rolfsii. Although induction of [beta]-glucuronidase activity was
observed in tissues that had not been exposed to these phytopathogens, the
greatest induction occurred in and around the site of fungal infection. The
increase in [beta]-glucuronidase activity closely paralleled the increase
in endogenous tobacco chitinase activity produced in response to fungal
infection. Thus, the chitinase 5B-gus A fusion gene may be used to analyze
the cellular and molecular details of the activation of the host defense
system during pathogen attack.
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