Plant Cell Plant Cell 20th
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (114)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bednarek, S. Y.
Right arrow Articles by Raikhel, N. V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bednarek, S. Y.
Right arrow Articles by Raikhel, N. V.
Agricola
Right arrow Articles by Bednarek, S. Y.
Right arrow Articles by Raikhel, N. V.

THE PLANT CELL, Vol 2, Issue 12 1145-1155, Copyright © 1990 by American Society of Plant Biologists

RESEARCH ARTICLES

A Carboxyl-Terminal Propeptide Is Necessary for Proper Sorting of Barley Lectin to Vacuoles of Tobacco

S. Y. Bednarek, T. A. Wilkins, J. E. Dombrowski and N. V. Raikhel
Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824-1312

Barley lectin is synthesized as a preproprotein with a glycosylated carboxyl-terminal propeptide (CTPP) that is removed before or concomitant with deposition of the mature protein in vacuoles. Expression of a cDNA clone encoding barley lectin in transformed tobacco plants results in the correct processing, maturation, and accumulation of active barley lectin in vacuoles [Wilkins, T.A., Bednarek, S.Y., and Raikhel, N.V. (1990). Plant Cell 2, 301-313]. The glycan of the propeptide is not essential for vacuolar sorting, but may influence the rate of post-translational processing [Wilkins, T.A., Bednarek, S.Y., and Raikhel, N.V. (1990). Plant Cell 2, 301-313]. To investigate the functional role of the CTPP in processing, assembly, and sorting of barley lectin to vacuoles, a mutant barley lectin cDNA clone lacking the 15-amino acid CTPP was prepared. The CTPP deletion mutant of barley lectin was expressed in tobacco protoplasts, suspension-cultured cells, and transgenic plants. In all three systems, the wild-type barley lectin was sorted to vacuoles, whereas the mutant barley lectin was secreted to the incubation media. Therefore, we conclude that the carboxyl-terminal domain of the barley lectin proprotein is necessary for the efficient sorting of this protein to plant cell vacuoles.


This article has been cited by other articles:


Home page
 J Exp BotHome page
E. L. Avila, M. Brown, S. Pan, R. Desikan, S. J. Neill, T. Girke, M. Surpin, and N. V. Raikhel
Expression analysis of Arabidopsis vacuolar sorting receptor 3 reveals a putative function in guard cells
J. Exp. Bot., April 1, 2008; 59(6): 1149 - 1161.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Zouhar, G. R. Hicks, and N. V. Raikhel
Sorting inhibitors (Sortins): Chemical compounds to study vacuolar sorting in Arabidopsis
PNAS, June 22, 2004; 101(25): 9497 - 9501.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
M. KIS, E. BURBRIDGE, I. W. BROCK, L. HEGGIE, P. J. DIX, and T. A. KAVANAGH
An N-terminal Peptide Extension Results in Efficient Expression, but not Secretion, of a Synthetic Horseradish Peroxidase Gene in Transgenic Tobacco
Ann. Bot., March 1, 2004; 93(3): 303 - 310.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
B.-H. Kang, J. S. Busse, C. Dickey, D. M. Rancour, and S. Y. Bednarek
The Arabidopsis Cell Plate-Associated Dynamin-Like Protein, ADL1Ap, Is Required for Multiple Stages of Plant Growth and Development
Plant Physiology, May 1, 2001; 126(1): 47 - 68.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
A. Vitale and G. Galili
The Endomembrane System and the Problem of Protein Sorting
Plant Physiology, January 1, 2001; 125(1): 115 - 118.
[Full Text]

Home page
 Plant Physiol.Home page
L. Frigerio, N. D. Vine, E. Pedrazzini, M. B. Hein, F. Wang, J. K.-C. Ma, and A. Vitale
Assembly, Secretion, and Vacuolar Delivery of a Hybrid Immunoglobulin in Plants
Plant Physiology, August 1, 2000; 123(4): 1483 - 1494.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
M. P. Does, P. M. Houterman, H. L. Dekker, and B. J.C. Cornelissen
Processing, Targeting, and Antifungal Activity of Stinging Nettle Agglutinin in Transgenic Tobacco
Plant Physiology, June 1, 1999; 120(2): 421 - 432.
[Abstract] [Full Text]

Home page
 Plant CellHome page
A. Vitale and J. Denecke
The Endoplasmic Reticulum—Gateway of the Secretory Pathway
PLANT CELL, April 1, 1999; 11(4): 615 - 628.
[Full Text]

Home page
 Plant CellHome page
L. Frigerio, M. de Virgilio, A. Prada, F. Faoro, and A. Vitale
Sorting of Phaseolin to the Vacuole Is Saturable and Requires a Short C-Terminal Peptide
PLANT CELL, June 1, 1998; 10(6): 1031 - 1042.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
M. H. Sato, N. Nakamura, Y. Ohsumi, H. Kouchi, M. Kondo, I. Hara-Nishimura, M. Nishimura, and Y. Wada
The AtVAM3 Encodes a Syntaxin-related Molecule Implicated in the Vacuolar Assembly in Arabidopsis thaliana
J. Biol. Chem., September 26, 1997; 272(39): 24530 - 24535.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 1990 by the American Society of Plant Biologists