THE PLANT CELL, Vol 2, Issue 12 1283-1290, Copyright © 1990 by American Society of Plant Biologists
RNA Editing of Wheat Mitochondrial ATP Synthase Subunit 9: Direct Protein and cDNA Sequencing
D. Begu, P. V. Graves, C. Domec, G. Arselin, S. Litvak and A. Araya
Institut de Biochimie Cellulaire et Neurochimie du Centre National de la Recherche Scientifique, 1, rue Camille Saint-Saens, 33077 Bordeaux Cedex, France
RNA editing of subunit 9 of the wheat mitochondrial ATP synthase has been
studied by cDNA and protein sequence analysis. Most of the cDNA clones
sequenced (95%) showed that editing by C-to-U transitions occurred at eight
positions in the coding region. Consequently, 5 amino acids were changed in
the protein when compared with the sequence predicted from the gene. Two
edited codons gave no changes (silent editing). One of the C-to-U
transitions generated a stop codon by modifying the arginine codon CGA to
UGA. Thus, the protein produced is 6 amino acids shorter than that deduced
from the genomic sequence. Minor forms of cDNA with partial or overedited
sequences were also found. Protein sequence and amino acid composition
analyses confirmed the results obtained by cDNA sequencing and showed that
the major form of edited atp9 mRNA is translated.
