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THE PLANT CELL, Vol 2, Issue 3 215-224, Copyright © 1990 by American Society of Plant Biologists

RESEARCH ARTICLES

A DNA-Binding Protein Factor Recognizes Two Binding Domains within the Octopine Synthase Enhancer Element

J. G. Tokuhisa, K. Singh, E. S. Dennis and W. J. Peacock
Division of Plant Industry, Commonwealth Scientific and Industrial Research Organization, GPO Box 1600, Canberra ACT 2601, Australia

A protein that binds to the enhancing element of the octopine synthase gene has been identified in nuclear extracts from maize cell suspension cultures. Two protein-DNA complexes are distinguishable by electrophoretic mobility in gel retardation assays. Footprint analyses of these low and high molecular weight complexes show, respectively, half and complete protection of the ocs-element DNA from cleavage by methidiumpropyl-EDTA[middle dot]FE(II). Two lines of evidence indicate that the element has two recognition sites, each of which can bind identical protein units. Elements that are mutated in one or the other half and form only the low molecular weight complex interfere with the formation of both the low and high molecular weight complexes by the wild-type element. Protein isolated from a complex with only one binding site occupied can bind to the wild-type ocs-element and generate complexes with protein occupying one or both binding sites. Occupation of both sites of the ocs-element is a prerequisite for transcriptional enhancement.


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J. A. Yunes, A. L. Vettore, M. J. da Silva, A. Leite, and P. Arruda
Cooperative DNA Binding and Sequence Discrimination by the Opaque2 bZIP Factor
PLANT CELL, November 1, 1998; 10(11): 1941 - 1956.
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Copyright © 1990 by the American Society of Plant Biologists