Plant Cell SoftGenetics
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (71)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lee, K. Y.
Right arrow Articles by Dunsmuir, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lee, K. Y.
Right arrow Articles by Dunsmuir, P.
Agricola
Right arrow Articles by Lee, K. Y.
Right arrow Articles by Dunsmuir, P.

THE PLANT CELL, Vol 2, Issue 5 415-425, Copyright © 1990 by American Society of Plant Biologists

RESEARCH ARTICLES

Homologous Recombination in Plant Cells after Agrobacterium-Mediated Transformation

K. Y. Lee, P. Lund, K. Lowe and P. Dunsmuir
DNA Plant Technology, 6701 San Pablo Avenue, Oakland, California 94608

A single amino-acid change in the acetolactate synthase (ALS) protein of tobacco confers resistance to the herbicide chlorsulfuron. A deleted, nonfunctional fragment from the acetolactate synthase gene, carrying the mutant site specifying chlorsulfuron resistance plus a closely linked novel restriction site marker, was cloned into a binary vector. Tobacco protoplasts transformed with Agrobacterium tumefaciens carrying this vector yielded chlorsulfuron-resistant colonies. DNA gel blot analysis of DNA from these colonies suggested that in three transformants homologous recombination had occurred between the endogenous ALS gene and the deleted ALS gene present in the incoming T-DNA. Plants were regenerated from these chlorsulfuron-resistant colonies, and in two of the transformants, genetic analysis of their progeny showed that the novel gene segregated as a single Mendelian locus. Possible models for the generation of these recombinant plants are discussed.


This article has been cited by other articles:


Home page
 Plant Cell PhysiolHome page
M. Endo, K. Osakabe, H. Ichikawa, and S. Toki
Molecular Characterization of True and Ectopic Gene Targeting Events at the Acetolactate Synthase Gene in Arabidopsis
Plant Cell Physiol., March 1, 2006; 47(3): 372 - 379.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Lloyd, C. L. Plaisier, D. Carroll, and G. N. Drews
Targeted mutagenesis using zinc-finger nucleases in Arabidopsis
PNAS, February 8, 2005; 102(6): 2232 - 2237.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
B. P. May, H. Liu, E. Vollbrecht, L. Senior, P. D. Rabinowicz, D. Roh, X. Pan, L. Stein, M. Freeling, D. Alexander, et al.
Maize-targeted mutagenesis: A knockout resource for maize
PNAS, September 30, 2003; 100(20): 11541 - 11546.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Kochevenko and L. Willmitzer
Chimeric RNA/DNA Oligonucleotide-Based Site-Specific Modification of the Tobacco Acetolactate Syntase Gene
Plant Physiology, May 1, 2003; 132(1): 174 - 184.
[Abstract] [Full Text] [PDF]

Home page
 Crop Sci.Home page
R. S. Lewis and E. A. Wernsman
Efforts to Initiate Construction of a Disease Resistance Package on a Designer Chromosome in Tobacco
Crop Sci., September 1, 2001; 41(5): 1420 - 1427.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Zhu, D. J. Peterson, L. Tagliani, G. St. Clair, C. L. Baszczynski, and B. Bowen
Targeted manipulation of maize genes in vivo using chimeric RNA/DNA oligonucleotides
PNAS, July 20, 1999; 96(15): 8768 - 8773.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
G. Shalev, Y. Sitrit, N. Avivi-Ragolski, C. Lichtenstein, and A. A. Levy
Stimulation of homologous recombination in plants by expression of the bacterial resolvase RuvC
PNAS, June 22, 1999; 96(13): 7398 - 7402.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Hallmann, A. Rappel, and M. Sumper
Gene replacement by homologous recombination in the multicellular green alga Volvox carteri
PNAS, July 8, 1997; 94(14): 7469 - 7474.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 1990 by the American Society of Plant Biologists