|
THE PLANT CELL, Vol 2, Issue 6 581-588, Copyright © 1990 by American Society of Plant Biologists
Identification and Molecular Characterization of Shrunken-2 cDNA Clones of Maize
M. R. Bhave, S. Lawrence, C. Barton and L. C. Hannah
Department of Vegetable Crops, University of Florida, Gainesville, Florida 32611
Mutation at the shrunken-2 (Sh2) locus of maize, a gene described more than
40 years ago, greatly reduces starch levels in the endosperm through its
effect on the starch synthetic enzyme ADP-glucose pyrophosphorylase, an
enzyme thought to be regulatory in this biosynthetic pathway. Although our
previous work has suggested that Sh2 is a structural gene for this enzyme,
we have also reported data compatible with Sh2 acting
post-transcriptionally. In this study, we took advantage of a transposable
element-induced Sh2 allele, its progenitor, and revertants to identify a
clone for this locus. Although the cloning and identification were done
independently of any knowledge concerning the product of this gene,
examination of the deduced amino acid sequence revealed much similarity to
known ADP-glucose pyrophosphorylase subunits of plants and bacteria,
including regions involved in substrate binding and activator binding.
Little sequence similarity, however, was found at the DNA level. These
observations provide direct evidence that Sh2 encodes a subunit for
endosperm ADP-glucose pyrophosphorylase. Analysis of several phenotypically
wild-type alleles arising from a mutable sh2-Ds allele revealed one
unexpected case in which DNA sequences of Sh2 were rearranged in comparison
with the progenitor Sh2. In contrast to wild type, the Ds-induced sh2
allele conditions at least two transcripts in the endosperm.
This article has been cited by other articles:
|
|
|
|
 
M. Cossegal, P. Chambrier, S. Mbelo, S. Balzergue, M.-L. Martin-Magniette, A. Moing, C. Deborde, V. Guyon, P. Perez, and P. Rogowsky
Transcriptional and Metabolic Adjustments in ADP-Glucose Pyrophosphorylase-Deficient bt2 Maize Kernels
Plant Physiology,
April 1, 2008;
146(4):
1553 - 1570.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. K. Boehlein, J. R. Shaw, J. D. Stewart, and L. C. Hannah
Heat Stability and Allosteric Properties of the Maize Endosperm ADP-Glucose Pyrophosphorylase Are Intimately Intertwined
Plant Physiology,
January 1, 2008;
146(1):
289 - 299.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. S. Kwak, S. R. Min, S.-M. Lee, K.-N. Kim, J. R. Liu, K.-H. Paek, J. S. Shin, and J. M. Bae
A Sepal-Expressed ADP-Glucose Pyrophosphorylase Gene (NtAGP) Is Required for Petal Expansion Growth in 'Xanthi' Tobacco
Plant Physiology,
September 1, 2007;
145(1):
277 - 289.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Kirchberger, M. Leroch, M. A. Huynen, M. Wahl, H. E. Neuhaus, and J. Tjaden
Molecular and Biochemical Analysis of the Plastidic ADP-glucose Transporter (ZmBT1) from Zea mays
J. Biol. Chem.,
August 3, 2007;
282(31):
22481 - 22491.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Georgelis, E. L. Braun, J. R. Shaw, and L. C. Hannah
The Two AGPase Subunits Evolve at Different Rates in Angiosperms, yet They Are Equally Sensitive to Activity-Altering Amino Acid Changes When Expressed in Bacteria
PLANT CELL,
May 1, 2007;
19(5):
1458 - 1472.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. D. Meyer, L. E. Talbert, J. M. Martin, S. P. Lanning, T. W. Greene, and M. J. Giroux
Field Evaluation of Transgenic Wheat Expressing a Modified ADP-Glucose Pyrophosphorylase Large Subunit
Crop Sci.,
February 6, 2007;
47(1):
336 - 342.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Rosti, H. Rudi, K. Rudi, H.-G. Opsahl-Sorteberg, B. Fahy, and K. Denyer
The gene encoding the cytosolic small subunit of ADP-glucose pyrophosphorylase in barley endosperm also encodes the major plastidial small subunit in the leaves
J. Exp. Bot.,
November 1, 2006;
57(14):
3619 - 3626.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. D. Yandeau-Nelson, Y. Xia, J. Li, M. G. Neuffer, and P. S. Schnable
Unequal Sister Chromatid and Homolog Recombination at a Tandem Duplication of the a1 Locus in Maize
Genetics,
August 1, 2006;
173(4):
2211 - 2226.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Ohdan, P. B. Francisco Jr, T. Sawada, T. Hirose, T. Terao, H. Satoh, and Y. Nakamura
Expression profiling of genes involved in starch synthesis in sink and source organs of rice
J. Exp. Bot.,
December 1, 2005;
56(422):
3229 - 3244.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. R. L. Linebarger, S. K. Boehlein, A. K. Sewell, J. Shaw, and L. C. Hannah
Heat Stability of Maize Endosperm ADP-Glucose Pyrophosphorylase Is Enhanced by Insertion of a Cysteine in the N Terminus of the Small Subunit
Plant Physiology,
December 1, 2005;
139(4):
1625 - 1634.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. M. Wilson, S. R. Whitt, A. M. Ibanez, T. R. Rocheford, M. M. Goodman, and E. S. Buckler IV
Dissection of Maize Kernel Composition and Starch Production by Candidate Gene Association
PLANT CELL,
October 1, 2004;
16(10):
2719 - 2733.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. W. Dudley, A. Dijkhuizen, C. Paul, S. T. Coates, and T. R. Rocheford
Effects of Random Mating on Marker-QTL Associations in the Cross of the Illinois High Protein x Illinois Low Protein Maize Strains
Crop Sci.,
July 1, 2004;
44(4):
1419 - 1428.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Guo and S. P. Moose
Conserved Noncoding Sequences among Cultivated Cereal Genomes Identify Candidate Regulatory Sequence Elements and Patterns of Promoter Evolution
PLANT CELL,
May 1, 2003;
15(5):
1143 - 1158.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
N. A. Eckardt
A New Twist on Transposons: The Maize Genome Harbors Helitron Insertion
PLANT CELL,
February 1, 2003;
15(2):
293 - 295.
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Datta, K. C. Chamusco, and P. S. Chourey
Starch Biosynthesis during Pollen Maturation Is Associated with Altered Patterns of Gene Expression in Maize
Plant Physiology,
December 1, 2002;
130(4):
1645 - 1656.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. A. Burton, P. E. Johnson, D. M. Beckles, G. B. Fincher, H. L. Jenner, M. J. Naldrett, and K. Denyer
Characterization of the Genes Encoding the Cytosolic and Plastidial Forms of ADP-Glucose Pyrophosphorylase in Wheat Endosperm
Plant Physiology,
November 1, 2002;
130(3):
1464 - 1475.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Yao, Q. Zhou, J. Li, H. Smith, M. Yandeau, B. J. Nikolau, and P. S. Schnable
From the Cover: Molecular characterization of meiotic recombination across the 140-kb multigenic a1-sh2 interval of maize
PNAS,
April 30, 2002;
99(9):
6157 - 6162.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. D. Smidansky, M. Clancy, F. D. Meyer, S. P. Lanning, N. K. Blake, L. E. Talbert, and M. J. Giroux
Enhanced ADP-glucose pyrophosphorylase activity in wheat endosperm increases seed yield
PNAS,
February 5, 2002;
99(3):
1724 - 1729.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. C. Hannah, J. R. Shaw, M. J. Giroux, A. Reyss, J.-L. Prioul, J.-M. Bae, and J.-Y. Lee
Maize Genes Encoding the Small Subunit of ADP- Glucose Pyrophosphorylase
Plant Physiology,
September 1, 2001;
127(1):
173 - 183.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-B. Choi, K.-H. Kim, I. H. Kavakli, S.-K. Lee, and T. W. Okita
Transcriptional Expression Characteristics and Subcellular Localization of ADP-Glucose Pyrophosphorylase in the Oil Plant Perilla frutescens
Plant Cell Physiol.,
February 1, 2001;
42(2):
146 - 153.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. H. Harn, J. M. Bae, S. S. Lee, S. R. Min, and J. R. Liu
Presence of Multiple cDNAs Encoding an Isoform of ADP-Glucose Pyrophosphorylase Large Subunit from Sweet Potato and Characterization of Expression Levels
Plant Cell Physiol.,
November 1, 2000;
41(11):
1235 - 1242.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Fu and H. K. Dooner
A Gene-enriched BAC Library for Cloning Large Allele-specific Fragments from Maize: Isolation of a 240-kb Contig of the bronze Region
Genome Res.,
June 1, 2000;
10(6):
866 - 873.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
T. W. Greene and L. C. Hannah
Enhanced stability of maize endosperm ADP-glucose pyrophosphorylase is gained through mutants that alter subunit interactions
PNAS,
October 27, 1998;
95(22):
13342 - 13347.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. W. Greene and L. C. Hannah
Maize Endosperm ADP–Glucose Pyrophosphorylase SHRUNKEN2 and BRITTLE2 Subunit Interactions
PLANT CELL,
August 1, 1998;
10(8):
1295 - 1306.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. Gao, J. Wanat, P. S. Stinard, M. G. James, and A. M. Myers
Characterization of dull1, a Maize Gene Coding for a Novel Starch Synthase
PLANT CELL,
March 1, 1998;
10(3):
399 - 412.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Chen, P. SanMiguel, A. C. de Oliveira, S.-S. Woo, H. Zhang, R. A. Wing, and J. L. Bennetzen
Microcolinearity in sh2-homologous regions of the maize, rice, and sorghum genomes
PNAS,
April 1, 1997;
94(7):
3431 - 3435.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. M. Stark, K. P. Timmerman, G. F. Barry, J. Preiss, and G. M. Kishore
Regulation of the Amount of Starch in Plant Tissues by ADP Glucose Pyrophosphorylase
Science,
October 9, 1992;
258(5080):
287 - 292.
[Abstract]
[PDF]
|
|
|
|
|
|
|
H. Yao, Q. Zhou, J. Li, H. Smith, M. Yandeau, B. J. Nikolau, and P. S. Schnable
From the Cover: Molecular characterization of meiotic recombination across the 140-kb multigenic a1-sh2 interval of maize
PNAS,
April 30, 2002;
99(9):
6157 - 6162.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
