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THE PLANT CELL, Vol 2, Issue 7 633-641, Copyright © 1990 by American Society of Plant Biologists
Nonlegume Hemoglobin Genes Retain Organ-Specific Expression in Heterologous Transgenic Plants
D. Bogusz, D. J. Llewellyn, S. Craig, E. S. Dennis, C. A. Appleby and W. J. Peacock
Division of Plant Industry, Commonwealth Scientific and Industrial Research Organization, GPO Box 1600, Canberra, ACT 2601, Australia
Hemoglobin genes from the nitrogen-fixing nonlegume Parasponia andersonii
and the related non-nitrogen-fixing nonlegume Trema tomentosa have been
isolated [Landsmann et al. (1986). Nature 324, 166-168; Bogusz et al.
(1988). Nature 331, 178-180]. The promoters of these genes have been linked
to a [beta]-glucuronidase reporter gene and introduced into both the
nonlegume Nicotiana tabacum and the legume Lotus corniculatus. Both
promoters directed root-specific expression in transgenic tobacco. When
transgenic Lotus plants were nodulated by Rhizobium loti, both promoter
constructs showed a high level of nodule-specific expression confined to
the central bacteroid-containing portion of the nodule corresponding to the
expression seen for the endogenous Lotus leghemoglobin gene. The T.
tomentosa promoter was also expressed at a low level in the vascular tissue
of the Lotus roots. The hemoglobin promoters from both nonlegumes,
including the non-nodulating species, must contain conserved cis-acting DNA
signals that are responsible for nodule-specific expression in legumes. We
have identified sequence motifs postulated previously as the
nodule-specific regulatory elements of the soybean leghemoglobin genes
[Stougaard et al. (1987). EMBO J. 6, 3565-3569].
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