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THE PLANT CELL, Vol 2, Issue 8 701-707, Copyright © 1990 by American Society of Plant Biologists
Preferential Transposition of the Maize Element Activator to Linked Chromosomal Locations in Tobacco
JDG. Jones, F. Carland, E. Lim, E. Ralston and H. K. Dooner
DNA Plant Technology, 6701 San Pablo Avenue, Oakland, California 94608
The autonomous maize transposon Activator (Ac) has been used in maize for
gene isolation by tagging and may prove similarly useful in other species.
To test the feasibility of gene tagging with heterospecific transposons, we
have examined three key genetic properties of a slightly modified Ac in
tobacco. First, we show that frequencies of germinal excision of this Ac
element from the antibiotic resistance gene streptomycin phosphotransferase
can be comparable with or slightly lower than in maize. Second, we show
that about half of the progeny carrying a germinal excision product also
carry a transposed Ac. Last, we have mapped transposed Ac locations
relative to the streptomycin transferase gene excision product and have
shown that as in maize Ac in tobacco preferentially transposes to
genetically linked sites.
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