THE PLANT CELL, Vol 2, Issue 8 785-793, Copyright © 1990 by American Society of Plant Biologists
Expression of a Maize Cell Wall Hydroxyproline-Rich Glycoprotein Gene in Early Leaf and Root Vascular Differentiation
V. Stiefel, L. Ruiz-Avila, R. Raz, M. P. Valles, J. Gomez, M. Pages, J. A. Martinez-Izquierdo, M. D. Ludevid, J. A. Langdale, T. Nelson and P. Puigdomenech
Departamento de Genetica Molecular, CID-CSIC, Jordi Girona 18, 08034 Barcelona, Spain
The spatial pattern of expression for a maize gene encoding a
hydroxyproline-rich glycoprotein (HRGP) was determined by in situ
hybridization. During normal development of roots and leaves, the
expression of the gene was transient and particularly high in regions
initiating vascular elements and associated sclerenchyma. Its expression
was also associated with the differentiation of vascular elements in a
variety of other tissues. The gene encoded an HRGP that had been extracted
from the cell walls of maize suspension culture cells and several other
embryonic and post-embryonic tissues. The gene was present in one or two
copies in different varieties of maize and in the related monocots teosinte
and sorghum. A single gene was cloned from maize using a previously
characterized HRGP cDNA clone [Stiefel et al. (1988). Plant Mol. Biol. 11,
483-493]. In addition to the coding sequences for the HRGP and an
N-terminal signal sequence, the gene contained a single intron in the
nontranslated 3[prime] end.
