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THE PLANT CELL, Vol 2, Issue 9 877-889, Copyright © 1990 by American Society of Plant Biologists
Cytochemical Analysis of Pollen Development in Wild-Type Arabidopsis and a Male-Sterile Mutant
S. M. Regan and B. A. Moffatt
Department of Biology, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1
Microsporogenesis has been examined in wild-type Arabidopsis thaliana and
the nuclear male-sterile mutant BM3 by cytochemical staining. The mutant
lacks adenine phosphoribosyltransferase, an enzyme of the purine salvage
pathway that converts adenine to AMP. Pollen development in the mutant
began to diverge from wild type just after meiosis, as the tetrads of
microspores were released from their callose walls. The first indication of
abnormal pollen development in the mutant was a darker staining of the
microspore wall due to an incomplete synthesis of the intine. Vacuole
formation was delayed and irregular in the mutant, and the majority of the
mutant microspores failed to undergo mitotic divisions. Enzyme activities
of alcohol dehydrogenase and esterases decreased in the mutant soon after
meiosis and were undetectable in mature pollen grains of the mutant. RNA
accumulation was also diminished. These results are discussed in relation
to the possible role(s) of adenine salvage in pollen development.
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