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THE PLANT CELL, Vol 2, Issue 9 905-912, Copyright © 1990 by American Society of Plant Biologists
Fertility Restoration Is Associated with Loss of a Portion of the Mitochondrial Genome in Cytoplasmic Male-Sterile Common Bean
S. A. Mackenzie and C. D. Chase
Department of Agronomy, Purdue University, West Lafayette, Indiana 47907
Restoration of pollen fertility to cytoplasmic male-sterile common bean by
nuclear gene Fr is accompanied by mitochondrial (mt) DNA rearrangements
within restored plants. These rearrangements are also observed upon
spontaneous cytoplasmic reversion to fertility. An mtDNA fragment of at
least 25 kilobases was lost from the genome upon restoration or reversion.
This fragment contained DNA segments that were not repeated elsewhere in
the genome and, therefore, were not detected within the genome upon
fertility restoration. This result suggested that the particular mtDNA
configuration absent from restored plants could not be maintained by a
constant process of recombination but rather by autonomous replication. No
evidence of excision of this region from the mt genome, in the form of a
junction fragment associating flanking DNA regions, was detected in fertile
restored plants. DNA gel blot hybridization of this mtDNA region, compared
with hybridization to related regions of the mitochondrial genome that
shared sequence homology, indicated that the mtDNA region associated with
sterility was present in lower copy number. These observations, as well as
the occurrence of similar or identical rearrangements upon spontaneous
cytoplasmic reversion, indicate that the restoration of pollen fertility
may be accompanied by loss of an independently replicating subgenomic DNA
molecule from the mitochondrial genome.
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