This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 20/11/2972    most recent tpc.107.056705v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Saez, A. Articles by Rodriguez, P. L. Search for Related Content PubMed PubMed Citation Articles by Saez, A. Articles by Rodriguez, P. L. Agricola Articles by Saez, A. Articles by Rodriguez, P. L.

HAB1–SWI3B Interaction Reveals a Link between Abscisic Acid Signaling and Putative SWI/SNF Chromatin-Remodeling Complexes in Arabidopsis^[C],[W]

Instituto de Biologia Molecular y Celular de Plantas, Consejo Superior de Investigaciones Cientificas–Universidad Politecnica de Valencia, ES-46022 Valencia, Spain

² Address correspondence to prodriguez{at}ibmcp.upv.es.

Abscisic acid (ABA) has an important role for plant growth, development, and stress adaptation. HYPERSENSITIVE TO ABA1 (HAB1) is a protein phosphatase type 2C that plays a key role as a negative regulator of ABA signaling; however, the molecular details of HAB1 action in this process are not known. A two-hybrid screen revealed that SWI3B, an Arabidopsis thaliana homolog of the yeast SWI3 subunit of SWI/SNF chromatin-remodeling complexes, is a prevalent interacting partner of HAB1. The interaction mapped to the N-terminal half of SWI3B and required an intact protein phosphatase catalytic domain. Bimolecular fluorescence complementation and coimmunoprecipitation assays confirmed the interaction of HAB1 and SWI3B in the nucleus of plant cells. swi3b mutants showed a reduced sensitivity to ABA-mediated inhibition of seed germination and growth and reduced expression of the ABA-responsive genes RAB18 and RD29B. Chromatin immunoprecipitation experiments showed that the presence of HAB1 in the vicinity of RD29B and RAB18 promoters was abolished by ABA, which suggests a direct involvement of HAB1 in the regulation of ABA-induced transcription. Additionally, our results uncover SWI3B as a novel positive regulator of ABA signaling and suggest that HAB1 modulates ABA response through the regulation of a putative SWI/SNF chromatin-remodeling complex.

This article has been cited by other articles:

				X. Saavedra, A. Modrego, D. Rodriguez, M. P. Gonzalez-Garcia, L. Sanz, G. Nicolas, and O. Lorenzo The Nuclear Interactor PYL8/RCAR3 of Fagus sylvatica FsPP2C1 Is a Positive Regulator of Abscisic Acid Signaling in Seeds and Stress Plant Physiology, January 1, 2010; 152(1): 133 - 150. [Abstract] [Full Text] [PDF]

				F. Vlad, S. Rubio, A. Rodrigues, C. Sirichandra, C. Belin, N. Robert, J. Leung, P. L. Rodriguez, C. Lauriere, and S. Merlot Protein Phosphatases 2C Regulate the Activation of the Snf1-Related Kinase OST1 by Abscisic Acid in Arabidopsis PLANT CELL, October 1, 2009; 21(10): 3170 - 3184. [Abstract] [Full Text] [PDF]

				C. Alexandre, Y. Moller-Steinbach, N. Schonrock, W. Gruissem, and L. Hennig Arabidopsis MSI1 Is Required for Negative Regulation of the Response to Drought Stress Mol Plant, July 1, 2009; 2(4): 675 - 687. [Abstract] [Full Text] [PDF]

				Z.-Q. Liu, J. Gao, A.-W. Dong, and W.-H. Shen A Truncated Arabidopsis NUCLEOSOME ASSEMBLY PROTEIN 1, AtNAP1;3T, Alters Plant Growth Responses to Abscisic Acid and Salt in the Atnap1;3-2 Mutant Mol Plant, July 1, 2009; 2(4): 688 - 699. [Abstract] [Full Text] [PDF]