This Article Full Text Full Text (PDF) PPT slides of all figures Supplemental Data All Versions of this Article: 20/11/3094    most recent tpc.108.061705v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Hiwatashi, Y. Articles by Hasebe, M. Search for Related Content PubMed PubMed Citation Articles by Hiwatashi, Y. Articles by Hasebe, M. Agricola Articles by Hiwatashi, Y. Articles by Hasebe, M.

Kinesins Are Indispensable for Interdigitation of Phragmoplast Microtubules in the Moss Physcomitrella patens^[W]

^a National Institute for Basic Biology, Okazaki 444-8585, Japan
^b School of Life Science, Graduate University for Advanced Studies (SOKENDAI), Okazaki 444-8585, Japan
^c ERATO, Japan Science and Technology Agency, Okazaki 444-8585, Japan

⁴ Address correspondence to mhasebe{at}nibb.ac.jp.

Microtubules form arrays with parallel and antiparallel bundles and function in various cellular processes, including subcellular transport and cell division. The antiparallel bundles in phragmoplasts, plant-unique microtubule arrays, are mostly unexplored and potentially offer new cellular insights. Here, we report that the Physcomitrella patens kinesins KINID1a and KINID1b (for kinesin for interdigitated microtubules 1a and 1b), which are specific to land plants and orthologous to Arabidopsis thaliana PAKRP2, are novel factors indispensable for the generation of interdigitated antiparallel microtubules in the phragmoplasts of the moss P. patens. KINID1a and KINID1b are predominantly localized to the putative interdigitated parts of antiparallel microtubules. This interdigitation disappeared in double-deletion mutants of both genes, indicating that both KINID1a and 1b are indispensable for interdigitation of the antiparallel microtubule array. Furthermore, cell plates formed by these phragmoplasts did not reach the plasma membrane in 20% of the mutant cells examined. We observed that in the double-deletion mutant lines, chloroplasts remained between the plasma membrane and the expanding margins of the cell plate, while chloroplasts were absent from the margins of the cell plates in the wild type. This suggests that the kinesins, the antiparallel microtubule bundles with interdigitation, or both are necessary for proper progression of cell wall expansion.

This article has been cited by other articles:

				A. M. Jovanovic, S. Durst, and P. Nick Plant cell division is specifically affected by nitrotyrosine J. Exp. Bot., March 1, 2010; 61(3): 901 - 909. [Abstract] [Full Text] [PDF]

				Y. Oda, A. Hirata, T. Sano, T. Fujita, Y. Hiwatashi, Y. Sato, A. Kadota, M. Hasebe, and S. Hasezawa Microtubules Regulate Dynamic Organization of Vacuoles in Physcomitrella patens Plant Cell Physiol., April 1, 2009; 50(4): 855 - 868. [Abstract] [Full Text] [PDF]