This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 20/11/3136    most recent tpc.108.061721v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by González-Lamothe, R. Articles by Brisson, N. Search for Related Content PubMed PubMed Citation Articles by González-Lamothe, R. Articles by Brisson, N. Pubmed/NCBI databases Protein UniGene Agricola Articles by González-Lamothe, R. Articles by Brisson, N.

The Transcriptional Activator Pti4 Is Required for the Recruitment of a Repressosome Nucleated by Repressor SEBF at the Potato PR-10a Gene^[W]

^a Department of Biochemistry, Université de Montréal, Montréal, Quebec, Canada H3C 3J7
^b Department of Biological Sciences, Brock University, St. Catharines, Ontario, Canada L2S 3A1
^c Centre de Recherche en Amélioration Végétale, Department of Biology, Université de Sherbrooke, Sherbrooke, Quebec, Canada J1K 2R1

² Address correspondence to cdespres{at}brocku.ca or normand.brisson{at}umontreal.ca.

Transcriptional reprogramming is critical for plant disease resistance responses. In potato (Solanum tuberosum), the marker gene PATHOGENESIS-RELATED-10a (PR-10a) is transcriptionally activated by pathogens, wounding, or elicitor treatment. Activation of PR-10a requires the recruitment of the activator Why1 to its promoter. In addition, PR-10a is negatively regulated by the repressor SEBF (for Silencer Element Binding Factor). Here, we show through a yeast two-hybrid screen that SEBF interacts with Pti4, which has been shown to be a transcriptional activator. SEBF recruits Pti4 via its consensus sequence–type RNA binding domain, while Pti4 is recruited to SEBF by means of its ethylene-response factor domain. In vivo plant transcription assays confirmed that SEBF interacts with Pti4 to form a repressosome, showing that Pti4 can also play a role in transcriptional repression. Chromatin immunoprecipitation revealed that both SEBF and Pti4 are recruited to the PR-10a promoter in uninduced conditions only and that the recruitment of Pti4 is dependent on the presence of SEBF, consistent with the fact that there is no Pti4 consensus binding site in PR-10a. Unexpectedly, we also demonstrated that recruitment of SEBF was dependent on the presence of Pti4, thereby explaining why SEBF, itself a repressor, requires Pti4 for its repressing function.

This article has been cited by other articles:

				M. Ikeda, N. Mitsuda, and M. Ohme-Takagi Arabidopsis WUSCHEL Is a Bifunctional Transcription Factor That Acts as a Repressor in Stem Cell Regulation and as an Activator in Floral Patterning PLANT CELL, November 1, 2009; 21(11): 3493 - 3505. [Abstract] [Full Text] [PDF]

				P. Boyle, E. Le Su, A. Rochon, H. L. Shearer, J. Murmu, J. Y. Chu, P. R. Fobert, and C. Despres The BTB/POZ Domain of the Arabidopsis Disease Resistance Protein NPR1 Interacts with the Repression Domain of TGA2 to Negate Its Function PLANT CELL, November 1, 2009; 21(11): 3700 - 3713. [Abstract] [Full Text] [PDF]