First published online March 7, 2008; 10.1105/tpc.107.054858
The Plant Cell 20:752-767 (2008)
© 2008 American Society of Plant Biologists
OPEN ACCESS ARTICLE
A MYB Transcription Factor Regulates Very-Long-Chain Fatty Acid Biosynthesis for Activation of the Hypersensitive Cell Death Response in Arabidopsis[W],[OA]
Sylvain Raffaelea,1,2,
Fabienne Vailleaua,1,3,
Amandine Légera,1,
Jérôme Joubèsb,
Otto Mierschc,
Carine Huarda,
Elisabeth Bléed,
Sébastien Mongrandb,
Frédéric Domergueb and
Dominique Robya,4
a Laboratoire des Interactions Plantes-Microorganismes, Unité Mixte de Recherche 2594/441, 31320 Castanet-Tolosan cedex, France
b Laboratoire de Biogenèse Membranaire, Unité Mixte de Recherche 5200, Université Victor Segalen Bordeaux 2, F-33000 Bordeaux cedex, France
c Institute of Plant Biochemistry, Halle, D-06120 Germany
d Laboratoire des Phytooxylipines, Institut de Biologie Moléculaire des Plantes, Unité Propre de Recherche 2357, 67083 Strasbourg Cedex, France
4 Address correspondence to roby{at}toulouse.inra.fr.
Plant immune responses to pathogen attack include the hypersensitive response (HR), a form of programmed cell death occurring at invasion sites. We previously reported on Arabidopsis thaliana MYB30, a transcription factor that acts as a positive regulator of a cell death pathway conditioning the HR. Here, we show by microarray analyses of Arabidopsis plants misexpressing MYB30 that the genes encoding the four enzymes forming the acyl-coA elongase complex are putative MYB30 targets. The acyl-coA elongase complex synthesizes very-long-chain fatty acids (VLCFAs), and the accumulation of extracellular VLCFA-derived metabolites (leaf epidermal wax components) was affected in MYB30 knockout mutant and overexpressing lines. In the same lines, a lipid extraction procedure allowing high recovery of sphingolipids revealed changes in VLCFA contents that were amplified in response to inoculation. Finally, the exacerbated HR phenotype of MYB30-overexpressing lines was altered by the loss of function of the acyl-ACP thioesterase FATB, which causes severe defects in the supply of fatty acids for VLCFA biosynthesis. Based on these findings, we propose a model in which MYB30 modulates HR via VLCFAs by themselves, or VLCFA derivatives, as cell death messengers in plants.
This article has been cited by other articles:

|
 |

|
 |
 
A. Kirik and M. B. Mudgett
SOBER1 phospholipase activity suppresses phosphatidic acid accumulation and plant immunity in response to bacterial effector AvrBsT
PNAS,
December 1, 2009;
106(48):
20532 - 20537.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
P. J. Seo, F. Xiang, M. Qiao, J.-Y. Park, Y. N. Lee, S.-G. Kim, Y.-H. Lee, W. J. Park, and C.-M. Park
The MYB96 Transcription Factor Mediates Abscisic Acid Signaling during Drought Stress Response in Arabidopsis
Plant Physiology,
September 1, 2009;
151(1):
275 - 289.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. D. Marks, J. P. Wenger, E. Gilding, R. Jilk, and R. A. Dixon
Transcriptome Analysis of Arabidopsis Wild-Type and gl3-sst sim Trichomes Identifies Four Additional Genes Required for Trichome Development
Mol Plant,
July 1, 2009;
2(4):
803 - 822.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. Okada, M. Nagabuchi, Y. Takamura, T. Nakagawa, K. Shinmyozu, J.-i. Nakayama, and K. Tanaka
Reconstitution of Arabidopsis thaliana SUMO Pathways in E. coli: Functional Evaluation of SUMO Machinery Proteins and Mapping of SUMOylation Sites by Mass Spectrometry
Plant Cell Physiol.,
June 1, 2009;
50(6):
1049 - 1061.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
W.-Q. Song, Y.-M. Qin, M. Saito, T. Shirai, F. M. Pujol, A. J. Kastaniotis, J. K. Hiltunen, and Y.-X. Zhu
Characterization of two cotton cDNAs encoding trans-2-enoyl-CoA reductase reveals a putative novel NADPH-binding motif
J. Exp. Bot.,
April 1, 2009;
60(6):
1839 - 1848.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
J. J. Reina-Pinto, D. Voisin, S. Kurdyukov, A. Faust, R. P. Haslam, L. V. Michaelson, N. Efremova, B. Franke, L. Schreiber, J. A. Napier, et al.
Misexpression of FATTY ACID ELONGATION1 in the Arabidopsis Epidermis Induces Cell Death and Suggests a Critical Role for Phospholipase A2 in This Process
PLANT CELL,
April 1, 2009;
21(4):
1252 - 1272.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
T. M. Quist, I. Sokolchik, H. Shi, R. J. Joly, R. A. Bressan, A. Maggio, M. Narsimhan, and X. Li
HOS3, an ELO-Like Gene, Inhibits Effects of ABA and Implicates a S-1-P/Ceramide Control System for Abiotic Stress Responses in Arabidopsis thaliana
Mol Plant,
January 1, 2009;
2(1):
138 - 151.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
Y.-C. Huang, L.-H. Su, G. A. Lee, P.-W. Chiu, C.-C. Cho, J.-Y. Wu, and C.-H. Sun
Regulation of Cyst Wall Protein Promoters by Myb2 in Giardia lamblia
J. Biol. Chem.,
November 7, 2008;
283(45):
31021 - 31029.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
L. Bach, L. V. Michaelson, R. Haslam, Y. Bellec, L. Gissot, J. Marion, M. Da Costa, J.-P. Boutin, M. Miquel, F. Tellier, et al.
The very-long-chain hydroxy fatty acyl-CoA dehydratase PASTICCINO2 is essential and limiting for plant development
PNAS,
September 23, 2008;
105(38):
14727 - 14731.
[Abstract]
[Full Text]
[PDF]
|
 |
|
|
|