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Pentatricopeptide Repeat Proteins with the DYW Motif Have Distinct Molecular Functions in RNA Editing and RNA Cleavage in Arabidopsis Chloroplasts^[W]

^a Department of Botany, Graduate School of Science, Kyoto University, Kyoto 606-8502 Japan
^b Australian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Perth 6009 WA, Australia
^c Graduate School of Agriculture, Organization for the Promotion of Advanced Research, Kyushu University, Fukuoka 812-8151, Japan
^d PRESTO, Japan Science and Technology, Kawaguchi 332-0012, Japan
^e Center for Gene Research, Nagoya University, Nagoya 464-8602, Japan
^f Plant Genome Network Research Team, RIKEN Plant Science Center, Yokohama 203-0045, Japan
^g Faculty of Agriculture, University of Shizuoka, Shizuoka 422-8529, Japan

¹ Address correspondence to shikanai{at}pmg.bot.kyoto-u.ac.jp.

The plant-specific DYW subclass of pentatricopeptide repeat proteins has been postulated to be involved in RNA editing of organelle transcripts. We discovered that the DYW proteins CHLORORESPIRATORY REDUCTION22 (CRR22) and CRR28 are required for editing of multiple plastid transcripts but that their DYW motifs are dispensable for editing activity in vivo. Replacement of the DYW motifs of CRR22 and CRR28 by that of CRR2, which has been shown to be capable of endonucleolytic cleavage, blocks the editing activity of both proteins. In return, the DYW motifs of neither CRR22 nor CRR28 can functionally replace that of CRR2. We propose that different DYW family members have acquired distinct functions in the divergent processes of RNA maturation, including RNA cleavage and RNA editing.

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