Plant Cell Hybrigenics The Protein Interactions Experts
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online January 9, 2009; 10.1105/tpc.108.063248

The Plant Cell 21:285-300 (2009)
© 2009 American Society of Plant Biologists

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow Author Profile
Right arrow All Versions of this Article:
21/1/285    most recent
tpc.108.063248v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in Plant Cell
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gerber, E.
Right arrow Articles by Bach, T. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gerber, E.
Right arrow Articles by Bach, T. J.
Agricola
Right arrow Articles by Gerber, E.
Right arrow Articles by Bach, T. J.

The Plastidial 2-C-Methyl-D-Erythritol 4-Phosphate Pathway Provides the Isoprenyl Moiety for Protein Geranylgeranylation in Tobacco BY-2 Cells[C],[W]

Esther Gerbera,1,2, Andréa Hemmerlina,1, Michael Hartmanna, Dimitri Heintza, Marie-Andrée Hartmanna, Jérôme Mutterera, Manuel Rodríguez-Concepciónb,3, Albert Boronatb, Alain Van Dorsselaerc, Michel Rohmerd, Dring N. Crowelle,4,5 and Thomas J. Bacha,5

a Institut de Biologie Moléculaire des Plantes (Centre National de la Recherche Scientifique, Unité Propre de Recherche 2357, associated with the Université Louis Pasteur), F-67083 Strasbourg, France
b Departament de Bioquímica i Biologia Molecular, Universitat de Barcelona, E-08028 Barcelona, Spain
c Université Louis Pasteur/Centre National de la Recherche Scientifique, Laboratoire de Spectrométrie de Masse Bio-Organique, Institut Pluridisciplinaire Hubert Curien, LC4-Unité Mixte de Recherche 7178, Ecole Européenne de Chimie, Polymères et Matériaux, F-67087 Strasbourg, France
d Université Louis Pasteur/Centre National de la Recherche Scientifique, Institut Le Bel, F-67070 Strasbourg, France
e Department of Biology, Indiana University–Purdue University, Indianapolis, Indiana 46202

5 Address correspondence to crowdrin{at}isu.edu or thomas.bach{at}bota-ulp.u-strasbg.fr.

Protein farnesylation and geranylgeranylation are important posttranslational modifications in eukaryotic cells. We visualized in transformed Nicotiana tabacum Bright Yellow-2 (BY-2) cells the geranylgeranylation and plasma membrane localization of GFP-BD-CVIL, which consists of green fluorescent protein (GFP) fused to the C-terminal polybasic domain (BD) and CVIL isoprenylation motif from the Oryza sativa calmodulin, CaM61. Treatment with fosmidomycin (Fos) or oxoclomazone (OC), inhibitors of the plastidial 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway, caused mislocalization of the protein to the nucleus, whereas treatment with mevinolin, an inhibitor of the cytosolic mevalonate pathway, did not. The nuclear localization of GFP-BD-CVIL in the presence of MEP pathway inhibitors was completely reversed by all-trans-geranylgeraniol (GGol). Furthermore, 1-deoxy-D-xylulose (DX) reversed the effects of OC, but not Fos, consistent with the hypothesis that OC blocks 1-deoxy-D-xylulose 5-phosphate synthesis, whereas Fos inhibits its conversion to 2-C-methyl-D-erythritol 4-phosphate. By contrast, GGol and DX did not rescue the nuclear mislocalization of GFP-BD-CVIL in the presence of a protein geranylgeranyltransferase type 1 inhibitor. Thus, the MEP pathway has an essential role in geranylgeranyl diphosphate (GGPP) biosynthesis and protein geranylgeranylation in BY-2 cells. GFP-BD-CVIL is a versatile tool for identifying pharmaceuticals and herbicides that interfere either with GGPP biosynthesis or with protein geranylgeranylation.


Related articles in Plant Cell:

A Plastidial Pathway for Protein Isoprenylation in Tobacco Cells
Nancy A. Eckardt
Plant Cell 2009 21: 13. [Full Text]  





HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2009 by the American Society of Plant Biologists